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CLINICAL CHEMISTRY (LEC) > KIDNEY FUNCTION TEST part 5 > Flashcards

KIDNEY FUNCTION TEST part 5 Flashcards

1
Q

What is the reagent used in Phophotungstic Acid (PTA) or Caraway method?

A

Phophotungstic Acid (PTA)

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2
Q

This substance is used to increase color and inhibit fading but considered to be hazardous?

A

Sodium Cyanide

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3
Q

What could be the alternative of Sodium cyanide?

A

Sodium Carbonate

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4
Q

If Sodium Carbonate is used, it is adjusted to what pH?

A

10.0 - 10.4

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5
Q

What is the positive result of PTA?

A

Tungsten blue

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6
Q

Tungsten blue is measured at what wavelength?

A

660 - 700 nm

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7
Q

What substances can lead to falsely elevated results?

A

Aspirin
Acetaminophen
Caffeine
Theophylline

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8
Q

This method determines the decrease in the UA concentration.

A

Blaunch and Koch (UV test with Uricase)

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9
Q

What happens in the first step of Blaunch and Koch (UV test with Uricase)?

A

Uricase converts UA to Allantoin and Hydrogen peroxide

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10
Q

What happens in the second step of Blaunch and Koch (UV test with Uricase)?

A

UA absorbs light at 285 nm.

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11
Q

What is the mode of reaction of Blaunch and Koch (UV test with Uricase)?

A

Decreasing

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12
Q

What is the relationship of decreased in absorbance in UV method to concentration of the UA?

A

Proportional

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13
Q

DHBS stands for?

A

Dihydroxy benzene sulfonate

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14
Q

This makes the product a yellow lutidine

A

Acetylacetone

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15
Q

This makes the product a tinder pink compound.

A

PAP

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16
Q

What is the color of copper neocuproine complex?

A

Yellow - orange

17
Q

These uses phosphotungstic acid chromophores.

A

Bittner method

18
Q

Two samples measurements is needed, ehat happens in the first measurement and second?

A

 First: treated with uricase to destroy UA
 Second: Uricase is absent

19
Q

Cuprous ions combined with neocuproine will yield a product of?

A

Copper neocuproine complex

20
Q

When Ferrous ions is reacted with TPTZ, what would be the product?

A

TPTZ method by Morin

21
Q

In the coupled enzymatic method, uricase is coupled with?

A

Peroxidase

22
Q

What substances can a false decrease result in BUA?

A

Reducing substances

23
Q

What is measured in Coupled enzymatic method?

A

Color change

24
Q

What are the 2 formation under Hyperurecemia?

A

Increased Formation
Decreased formation

25
Q

Under decreased Excreation of Hyperurecemia, what is the drug therapy and poisons for secondary?

A

Salicylate
Heavy metal

CLINICAL CHEMISTRY (LEC) (23 decks)

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