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Flashcards in Microscopy Deck (12):
1

Fungal Stains?

1. Lactophenol cotton blue
-Histoplasma
2. GMS
-Aspergillus
-Pneumocystis carinii
-blastomyces dermatitidis
3. PAS
-aspergillus in lung
4. Mucin
-cryptococcus in lung

2

What do you use Warthin-Starry?

spirochets-cat scratch disease

3

What stains do you use for parasite?

1. wright's giemsa
-malaria
-trypanosomes
2. wet mounts
giardia
3. trichrome
-giardia
4. modified acid fast
-nocardia

4

What does auramine rhodamine stain for?

mycobacteria

5

What does calcofluor white stain for?

fungi

6

What does fluorescein conjugated antibodies?

viruses, bordetella, legionella, pneumocystis

7

darkfield

must be alive
-spirochetes

8

electron microscopy

-viruses microsporum

9

Grain Stain Procedure

1. cells on the slide
2. primary stain
-crystal violet
3. mordant
-grams iodine
4. decolorize
-alcohol or acetone
5. counterstain
-safranin

10

What is the clinical utility of a gram stain?

1. direct examination of clinical material to make an initial diagnosis
2. determine the quality of the specimen
3. suggest an unusual organism or provide early presumptive bacterial identification
4. verify the clinical relevance of the culture

11

Best specimens for performing direct exam?

1. strep
-diplococci, positive
2. gonorrhea
-gram negative, intracellular
3. meningitis
-flat adjacent side-gram negative
4. cryptococcus in CSF
5. candida
6. bacterial vaginosis

12

What does acid fast stain?

mycobacteria
nocardia-bacteria
cryptosporidia-parasite
cyclospora-parasite