Flashcards in ABXR 2 Deck (9)
Does multiple resistance occur independently?
No already exists together
-bacterial cells can acquire genes for resistance or for virulence by obtaining large pieces of DNA from other bacteria or bacteriophage
What is transformation?
1. naked DNA released by donor cell (lysis)
2. uptake of DNA by recipient cell (competence)
How does transformation work?
-when cell senses pheromones it becomes competent
-expresses machinery for getting in DNA
1. DNA binding proteins
2. nucleases-single stranded
3. pore to bring in the single stranded DNA
4. DNA binding to proteins until it can reach the homologous portion in the chromosome where it can bind and homologously recombine
What are the three fates of the exogenote?
1. degraded(lost to nucleases)
2. circularized (lost to dilution)
3. recombined (stably retained)
What is conjugation
-r plasmid in donor
-r plasmid in recipient
What is the clinical relevance of conjugation?
1. rapid dissemination of drug resistance via conjugal R plasmid or conjugal transposons
2. mediates inter-species transfer transfer from non-pathogenic bacteroides to pathogenic shigella or e coli
What is transduction?
mediated by bacteriophage
1) protein capsid
2) DNA or RNA
-phage can carry virulence factors
ex. beta toxin by diphtheria or cholera toxin by vibrio cholerae CTX phage
What is the host chromosome cell that carries the prophage called?