Module 12: Immunological Testing Methods Obj. 3 SA Flashcards Preview

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Flashcards in Module 12: Immunological Testing Methods Obj. 3 SA Deck (5)
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1

Agglutination

formation of cross linked Ag-Ab lattices in which Ag is on the surface of a cell or other insoluble particle

2

3 major agglutination reactions

direct
indirect (passive)
Viral

3

Direct agglutination

for naturally occurring Ag on surface of a cell
ex. Anti A antisera attaches to A Ag on RBC

ex. Mycoplasma pneumoniae Ab attaches to I antigen on RBC

ex. Heterophile Ab produced during infection mononucleosis will agglutinate sheep red cells

4

Indirect (Passive) Agglutination

Ag is actively (by manufacturer) attached to the carrier particle (charcoal, latex, gelatine, sheep rbc)

Ex. Serological producers to diagnose syphilis
ex. Rubella testing, Rheumatoid arthritis may produce an Ab called RF; Anti-streptolysin O is an antibody produced by patients post infection with beta hemolytic group A Strep.; Detect CRP using latex particles with anti-CRP attached

5

Viral hemagglutination **Inhibition**

indirect way to detect presence of viral Ab in patient
POS REACTION:
Viral reagent + pt sample WITH Ab + REAGENT RBC = NO agglutination

NEG REACTION:
viral reagent + pt sample W/O Ab + reagent RBC = Agglutination