Module 12: Immunological Testing Methods Obj. 5 Flashcards Preview

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Flashcards in Module 12: Immunological Testing Methods Obj. 5 Deck (5)
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1

4 main groups of labels used in immunoassays

Radioactive
Enzyme
Fluorescent
Chemiluminescent

2

Radioimmunoassay (RIA)

utilize atoms with unstable nuclei that emit radiation that can be detected using a counter

3

Enzyme immunoassay (EIA)

utilize non-isotopic labels
Enzyme labeled Ab or Ag may be used

4

Fluorescent Immunoassay (FIA)

molecules which will absorb the energy of a photon of one wavelength and emit a photon of a slightly longer (lower energy) wavelength

5

Chemiluminescence immunoassay (CIA)

substrates are modules which emit a photon of light as a result of a chemical reaction
ex. Luminol