Module 12: Immunological Testing Methods Obj. 4 SA Flashcards Preview

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Flashcards in Module 12: Immunological Testing Methods Obj. 4 SA Deck (1)
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Stages of complement fixation to detect Ag-Ab reactions

Pt serum with Ab + Known Reagent Ag + Complement = (binding of complement to pt Ab) + sheep RBC with Ab attached = no hemolysis

Pt serum w/o Ab + Known reagent Ag + complement = no binding of complement (still available to bind to sensitized sheep rbc) + Sheep RBC = lysis