Neisseriaceae Flashcards

1
Q

A gram positive (+) diplococcus

A

Streptococcus pneumoniae

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2
Q

Gram negative (-) diplococci

A
  • Neisseria spp.
  • Moraxella spp.
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3
Q

Gram negative (-) curved bacilli

A
  • Vibrio spp.
  • Plesiomonas spp.
  • Aeromonas spp.
  • Campylobacter spp.
  • Helicobacter spp.
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4
Q

When the organism appears to be gram negative (-), the first test to conduct is __________.

A

Oxidase Test

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5
Q

All gram negative (-) diplococci and curved bacilli are ______.

A

oxidase positive (+)

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6
Q

Neisseria spp. belong to the family _________.

A

Neisseriaceae

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7
Q

What are some generas under the family Neisseriaceae?

A

MNEMONIC: EKS
* Eikenella spp.
* Kingella spp.
* Simonsiella spp.

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8
Q

How are Neisseria spp. different from other generas of Neisseriaceae?

A
  1. Neisseria spp. are OXIDASE and CATALASE (+)
  2. In terms of morphology nd gram stain reaction, they are GRAM (-) DIPLOCOCCI.

Other generas are (-) for catalase, and are bacilli.

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9
Q

How can we differentiate Neisseria spp. from other generas of the family Neisseriaceae?

A

By performing a CATALASE TEST

Neisseria spp. are (+), while others are (-)

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10
Q

What is the aerotolerance of Neisseria spp.?

A

Capnophilic, Aerobic (in terms of metabolism)

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11
Q

Neisseria spp. can grow _______ if alternative electron receptors are available.

A

anaerobically

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12
Q

Neisseria spp. can grow anaerobically if ____________ are available.

A

alternative electron receptors (e.g., nitrites)

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13
Q

Are Neisseria spp. motile?

A

No

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14
Q

Are Neisseria spp. spore-forming?

A

No

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15
Q

Which Neisseria spp. are gram negative (-) bacilli?

A

MNEMONIC: EWBa
* N. elongata
* N. weaveri
* N. bacilliformis

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16
Q

What is the specific shape of Neisseria spp.?

A

Kidney-bean shaped diplococci

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17
Q

Which Neisseria spp. are oxidase (+) but are catalase (-)?

A

MNEMONIC: EBa
* N. elongata
* N. bacilliformis

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18
Q

Neisseria spp. are normal inhabitants of the _______.

A

Upper Respiratory Tract (URT)

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19
Q

Which Neisseria spp. are not considered a normal flora?

A

MNEMONIC: GoAn
* N. gonorrhoeae
* N. animaloris

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20
Q

The presence of these bacteria can be pathogenic.

A
  • N. gonorrhoeae
  • N. animaloris
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21
Q

Are Neisseria spp. fastidious?

A

Yes

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22
Q

What are the requirements for Neisseria spp. in order for them to grow?

A

CO2 and iron

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23
Q

These bacteria compete for the iron in our body.

A

N. gonorrhoeae and N. meningitidis

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24
Q

The bacterial cell of Neisseria spp. have a receptor for ______.

A

Transferrin

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25
Q

It is the transport protein of iron, which cleans the iron in our body.

A

Transferrin

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26
Q

How does transferrin supply iron to Neisseria spp.?

A

By attaching to the receptor on the bacterial cell

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27
Q

These species are found to be pathogenic for humans, and are found associated inside polymorphonuclear cells (PMNs).

A

N. gonorrhoeae and N. meningitidis

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28
Q

How is N. gonorrhoeae transmitted?

A

By sexual intercouse (STI)

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29
Q

How is N. meningitidis transmitted?

A

By repiratory droplets

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30
Q

What is the optimum temperature for Neisseria spp.?

A

32 - 37 C

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31
Q

Enumerate the virulence factors associated with Neisseria spp.

A
  1. Receptor for Transferrin
  2. Capsule
  3. Pili
  4. Cell membrane proteins
  5. Lipooligosaccharide (LOS) or Endotoxin
  6. Immunoglobulin A protease
  7. Lip (H8)
  8. Ferric-binding protein (FBP)
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32
Q

Which Neisseria sp. is encapsulated?

A

N. meningitidis

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33
Q

The basis of virulence is determined by the presence or absence of ______.

A

Pili (fimbriae)

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34
Q

5 Colonial Types of N. gonorrhoeae

A

T1, T2, T3, T4, T5

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35
Q

Which colonial type of N. gonorrhoeae is virulent?

A

T1 and T2

They are virulent because PILI is PRESENT

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36
Q

Which colonial type of N. gonorrhoeae is avirulent?

A

T3, T4, and T5

They are avirulent because PILI is ABSENT

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37
Q

Which cell membrane protein is encoded by the gene “por”?

A

Protein I

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38
Q

Protein I is encoded by the gene ______.

A

por

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39
Q

Which virulence factor?

It forms a channel for nutrients to pass into and waste products to exit the cell.

A

Protein I

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40
Q

Two Types of Por Gene

A

Por A and Por B

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41
Q

Types of Por Gene

Which por gene can be found in N. meningitidis?

A

Por A and Por B

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42
Q

Types of Por Gene

Which por gene can be found in N. gonorrhoeae?

A

Por B

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43
Q

Which virulence factor?

It facilitates adherence to phagocytic and epithelial cells.

A

Protein II (Opa)

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44
Q

Which virulence factor?

It blocks the bacteriocidal effect of host IgG.

A

Protein III (Reduction Modified Protein)

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45
Q

Which virulence factor?

It resembles the human cell membrane glycosphingolipid.

A

Lipooligosaccharide (LOS) or Endotoxin of N. gonorrhoeae

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46
Q

Which virulence factor?

It cleaves the IgA on mucosal surfaces.

A

Immunoglobulin A (IgA) Protease

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47
Q

Immunoglobulin A (IgA) Protease can also be found in _______.

A

S. pneumoniae and H. influenzae

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48
Q

Which virulence factor?

A surface exposed protein that is heat-modifiable like Opa.

A

Lip (H8)

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49
Q

Which virulence factor?

It is expressed when the available iron supply is limited.

A

Ferric-binding protein (FBP)

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50
Q

Which carbohydrate is the only one that Neisseria gonorrhoeae oxidizes?

A

Glucose

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51
Q

How can we differentiate Neisseria gonorrhoeae from other species of Neisseria?

A

By performing Carbohydrate Utilization Test

Wherein GLUCOSE is the only carbohydrate that N. gonorrhoeae oxidi

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52
Q

Which strain of N. gonorrhoeae requires arginine, hypoxanthine, and uracil?

A

AHU Strains

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53
Q

AHU stands for ____.

A

Arginine, Hypoxanthine, Uracil

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54
Q

AHU strains are found in _________.

A

3-5% asymptomatic males with gonorrhea

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55
Q

_____ are responsible for the asymptomatic manifestation of gonorrhea.

A

AHU Strains

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56
Q

AHU strains are found in ____ of asymptomatic males.

A

3-5%

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57
Q

Neisseria gonorrhoeae

Commonly affected sites in Males

A

Urethra

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58
Q

Neisseria gonorrhoeae

Commonly affected sites in Females

A

Cervix

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59
Q

Neisseria gonorrhoeae is only found on mucous membranes of the ___________ at the time of infection.

A

genitalia, anorectal area, oropharynx, and conjunctiva

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60
Q

An acute pyogenic infection of the non-ciliated columnar and transitional epithelium.

A

Gonorrhea

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61
Q

Gonorrhea is acquired through ________.

A

sexual contact

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62
Q

Gonorrhea is also known as the ______.

A

“Flow of Seed”

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63
Q

Gonorrhea is also known as _____ which came from the French word “clapoir” meaning brothel.

A

“The Clap”

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64
Q

Clapoir in French means _______.

A

Brothel

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65
Q

Incubation period of Gonorrhea

A

2-7 days

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66
Q

Neisseria gonorrhoeae

Clinical Infections in Males

A
  1. Acute urethritis
  2. Dysuria
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67
Q

Neisseria gonorrhoeae

Clinical Infections in Females

A
  1. Dysuria
  2. Cervical discharge
  3. Lower abdominal pain
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68
Q

Neisseria gonorrhoeae

Percentage of Asymptomatic Females

A

50%

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69
Q

Neisseria gonorrhoeae

Percentage of Women Experiencing Concurrent Anorectal Infections

A

30-60%

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70
Q

Neisseria gonorrhoeae

The most common and known complication in Women

A

Perihepatitis

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71
Q

Perihepatitis is also known as ________.

A

Fitz-Hugh-Curtis Syndrome

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72
Q

Neisseria gonorrhoeae

Other complications in Women

A

Pelvic inflammatory disease, sterility, ectopic pregnancy

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73
Q

A gonococcal eye infection transmitted vertically from the mother to baby.

A

Ophtalmia neonatorum

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74
Q

What are the 2 kinds of Vertical Transmission?

A
  1. Transplacental
  2. Connatal
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75
Q

N. gonorrhoeae is sensitive to _________.

A
  • extreme temperatures
  • disinfectants
  • cotton swabs
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76
Q

Specimens for N. gonorrhoeae

A

Pus and secretions

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77
Q

Neisseria gonorrhoeae

Specimen of choice for Men

A

Urethra

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78
Q

Neisseria gonorrhoeae

Specimen of choice for Women

A

Endocervix

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79
Q

Which swabs are preferred for collecting N. gonorrhoeae?

A

Dacron or Rayon swabs

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80
Q

These are not used for they are toxic and inhibitory for N. gonorrhoeae.

A

Calcium alginate and cotton swabs

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81
Q

A bedside culture media

A

Direct Plating

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82
Q

These are used whenever direct plating is not possible.

A

Transport systems such as JEMBEC, Gono-Pak, and Transgrow

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83
Q

JEMBEC stands for _________.

A

James E. Martin Biological Environmenal Chamber

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84
Q

Which pattern is done when streaking JEMBEC?

A

Z pattern

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85
Q

This is used when there is no JEMBEC or other transport systems available.

A

Amies Medium with Charcoal

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86
Q

Amies medium with charcoal is plated within _______ after collection.

A

6 hours

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87
Q

If there is no discharge, the swab is inserted _____ deep into the anterior urethra.

A

2 cm

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88
Q

If there is no discharge, the swab is inserted _____ deep into the anal canal.

A

4-5 cm

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89
Q

Neisseria gonorrhoeae

Urogenital specimens appearance under direct microscopic examination

A

Gram (-) intracellular kidney-shaped diplococci

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90
Q

Gram stain is not recommended for _______.

A

pharyngeal specimens

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91
Q

Why is gram stain not recommended for pharyngeal specimens?

A

Because the oral part of our body contains many Neisseria microbiota which can be hard to distinguish.

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92
Q

What does it mean when you see more than 5 polymorphonuclear neutrophils with no bacteria?

A

Non-gonoccocal urethritis

Caused by: Chlamydia trachomatis or Ureaplasma urealyticum

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93
Q

Non-gonococcal urethritis is caused either by _______.

A

Chlamydia trachomatis or Ureaplasma urealyticum

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94
Q

Medium of Choice for N. gonorrhoeae

A

CHOC Agar (CAP)

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95
Q

Other media used for N. gonorrhoeae

A

Trypticase Soy Agar with 5% sheep blood

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96
Q

TRUE OR FALSE.
N. gonorrhoeae can grow on Sheep Blood Agar or BAP.

A

False

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97
Q

Selective Media for the Isolation of N. gonorrhoeae and N. meningitidis

A
  1. Thayer-Martin
  2. Modified Thayer-Martin
  3. Martin Lewis
  4. New York City
  5. GC-LECT
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98
Q

Thayer-Martin inhibitory agents

A
  • Vancomycin
  • Colistin
  • Nystatin
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99
Q

Vancomycin is an inhibitor of _______ organisms.

A

Gram positive (+)

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100
Q

Colistin is an inhibitor of _______ organisms.

A

Gram negative (-)

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101
Q

Nystatin is an inhibitor of _______.

A

Yeasts

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102
Q

Anisomycin is an inhibitor of _______.

A

Yeasts

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103
Q

Amphotericin B is an inhibitor of _______.

A

Yeasts

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104
Q

Trimethoprim is an inhibitor of _______.

A

Swarming proteus spp.

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105
Q

Lincomycin is an inhibitor of ______ organisms.

A

Gram positive (+)

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106
Q

Modified Thayer-Martin inhibitory agents

A
  • Vancomycin
  • Colistin
  • Nystatin
  • Trimethoprim
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107
Q

Martin-Lewis inhibitory agents

A
  • Vancomycin
  • Colistin
  • Anisomycin
  • Trimethoprim
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108
Q

New York City inhibitory agents

A
  • Vancomycin
  • Colistin
  • Amphotericin B
  • Trimethoprim
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109
Q

GC-LECT inhibitory agents

A
  • Vancomycin
  • Lincomycin
  • Colistin
  • Amphotericin B
  • Trimethoprim
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110
Q

Standard incubation environment for Neisseria spp.

A

35 C in 3-5% CO2 atmosphere

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111
Q

A brand of candle jar, wherein instead of using a candle to generate CO2, CO2 pouch is used.

A

Gaspak Jar

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112
Q

Colonial apperance of Neisseria gonorrhoeae

A

Small, grayish-white, convex, translucent, shiny colonies with either smooth or irregular margins.

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113
Q

Microscopic morphology of Neisseria spp.

A

Gram negative (-) diplococci

114
Q

Reagent for Oxidase Test

A

1% dimethyl-p-phenylenediamine-dihydrochloride or tetramethyl-p-phenylenediamine dihydrochloride

115
Q

Positive result for Oxidase Test

A

Purple color within 10 seconds

116
Q

Which culture media is used in Carbohydrate Utilization Test?

A

Cystine Trypticase Agar (CTA)

117
Q

pH indicator for Carbohydrate Utilization Test

A

phenol red

118
Q

Positive result for Carbohydrate Utilization Test

A

(+) Acid: Yellow color produced in 24-72 hours

119
Q

Incubation period of Neisseria spp.

A

24 - 72 hours

120
Q

Negative result for Carbohydrate Utilization Test

A

(-) No Acid: Red Color

121
Q

Two principles of Immunologic Assays

A
  1. Coagglutination using monoclonal antibodies (against gonococcal protein I)
  2. Fluorescent antibody testing
122
Q

Positive result for Immunologic Assays

A

(+) Agglutination

123
Q

Matrix-Assisted Laser is also known as ________.

A

Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

124
Q

It is the most advanced identification system for the identification of different bacteria.

A

Matrix-Assisted Laser (MALDI-TOF MS)

125
Q

It identifies infectious pathogens by defining the unique protein signatures of the organism.

A

Matrix-Assisted Laser (MALDI-TOF MS)

126
Q

It detects the enzymes that hydrolyze colorless substrates and produce colored end products.

A

Gonochek II

127
Q

It treats only the strains that are isolated from selective media.

A

Chromogenic Substrates

128
Q

It allows for the identification of strains isolated on selective or non-selective media.

A

Multitest Methods

129
Q

A commensall as well as an invasive pathogen.

A

Neisseria meningitidis

130
Q

How can N. meningitidis be invasive?

A

It is a normal flora of the URT. When it gains access to the bloodstream, it can go to the CNS and cause meningitis.

131
Q

N. meningitidis is the etiologic agent of _______.

A

Meningitis and Meningococcemia

132
Q

Serogroups of N. meningitidis

A

A, B, C, Y, and W-135

133
Q

Capsular component of N. meningitidis

A

Sialic acid moieties

134
Q

Two Common Clinical Infections caused by N. meningitidis

A
  1. Meningitis
  2. Meningococcemia
135
Q

A common complication of meningococcemia.

A

Hemorrhage in the adrenal glands (Waterhouse-Friderichsen Syndrome)

136
Q

A syndrome characterized by bleeding in the adrenal glands.

A

Waterhouse-Friderichsen Syndrome

137
Q

It is characterized by an abrupt onset of frontal headache, stiff neck (nuchal rigidity), confusion, and photophobia.

A

Meningitis

138
Q

Specimens for N. meningitidis

A

CSF, blood, nasopharyngeal swabs, aspirates, joint fluids, etc.

139
Q

An anticoagulant in the blood that is inhibitory for Neisseria spp.

A

0.025% Sodium Polyanethole Sulfonate (SPS)

140
Q

What is done when there are no other anticoagulants?

A

Gelatin is added to neutralize the effect of SPS

141
Q

Neisseria meningitidis

Direct Microscopic Examination

A

Intracellular and Extracellular gram negative (-) diplococci

142
Q

Neisseria meningitidis

Culture and Incubation

A
  • SBA/BAP and CHOC/CAP Agar
  • Same atmospheric conditions as N. gonorrhoeae
  • Examined daily for 72 hours
143
Q

Neisseria meningitidis

Colonial Appearance

A

Medium, smooth, round, moist, gray to white, encapsulated, mucoid, may be greenish cast in agar underneath colonies.

144
Q

Neisseria meningitidis

Identification

A
  • Oxidase and Catalase (+)
  • Carbohydrate Utilization Test (Maltose, Glucose)
  • Molecular Techniques
  • MALDI-TOF MS
145
Q

Which carbohydrate/s is/are utilized by N. meningitidis?

A

Maltose and Glucose

146
Q

Neisseria meningitidis

Drug of Choice (DOC)

A

Penicillin

147
Q

Neisseria meningitidis

Treatment for Meningococcemia

A

Third generation cephalosporin

148
Q

Neisseria meningitidis

Chemoprophylaxis

A

Rifampin, Ciprofloxacin

149
Q

Neisseria meningitidis

Treatment for Ciprofloxacin resistance

A

Azithromycin

150
Q

Neisseria cinerea

Glucose oxidation result in CTA

A

Negative (-)

151
Q

TRUE OR FALSE.
Neisseria cinerea cannot grow on SBA.

A

False

152
Q

Neisseria cinerea

Result in hydroxyprolyl aminopeptidase reaction

A

Positive (+)

153
Q

Neisseria cinerea

Reduction of nitrate

A

Negative (-)

154
Q

Neisseria cinerea

DNAse reaction result

A

Negative (-)

155
Q

Which characteristics of Neisseria cinerea differentiates it from Moraxella catarrhalis?

A

Negative DNAse reaction

156
Q

Which characteristic of Neisseria cinerea differentiates it from Neisseria flavescens?

A

Lack of yellow pigment production

157
Q

Neisseria lactamica is typically found in the _______.

A

Nasopharynx of infants and children

158
Q

______ the only Neisseria sp. that utilizes lactose.

A

Neisseria lactamica

159
Q

TRUE OR FALSE.
N. gonorrhoeae exhibits a delayed lactose utilization.

A

False

Correct Ans: Neisseria lactamica

160
Q

N. lactamica is often confused with ______.

A

Neisseria meningitidis

161
Q

Neisseria lactamica

Ortho-nitrophenyl-β-D galactopyranoside result

A

Positive (+)

162
Q

A test used to detect the presence of delayed lactose fermeners.

A

ONPG Test

163
Q

ONPG stands for ___________.

A

Ortho-nitrophenyl-β-D galactopyranoside

164
Q

It is large, often adherent to the agar, and very mucoid.

A

Neisseria mucosa

165
Q

N. mucosa is documented to cause _______ in children.

A

Pneumonia

166
Q

N. mucosa has the same carbohydrate pattern as _______.

A
  • N. sicca
  • N. subflava biovar. perflava
167
Q

TRUE OR FALSE.
N. mucosa has the ability to reduce nitrite to nitrogen gas.

A

True

168
Q

TRUE OR FALSE.
N. mucosa is capable of pigment production.

A

False

169
Q

Dry, wrinkled, adherent, and breadcrumb-like

A

Neisseria sicca

170
Q

Sicca means ____.

A

dry

171
Q

Two most common Neisseria spp. found in the respiratory tract

A
  • N. sicca
  • N. subflava biovar.perflava
172
Q

Neisseria sicca causes _________.

A

Endocarditis

173
Q

A variant of Neisseria mucosa

A

Neisseria sicca

174
Q

“Less yellow”

A

Neisseria subflava

175
Q

TRUE OR FALSE.
N. subflava is part of the upper respiratory microbiota.

A

True

176
Q

N. subflava is capable of causing serious infections such as _______.

A

bacteremia, meningitis, septicemia

177
Q

N. subflava resembles the infections caused by ________.

A

N. meningitidis

178
Q

N. subflava has a reduced sensitivity to _________.

A

penicillin, cefixime, ciprofloxacin

179
Q

N. subflava has a reduced ________ to penicillin, cefixime, and ciprofloxacin.

A

sensitivity

180
Q

________ has a reduced sensitivity to penicillin, cefixime, and ciprofloxacin.

A

N. subflava

181
Q

What are the 3 subspecies of Neisseria elongata?

A
  1. Neisseria elongata subsp. elongata
  2. Neisseria elongata subsp. glycolytica
  3. Neisseria elongata subsp. nitroreducens
182
Q

TRUE OR FALSE.

N. elongata is not a commensal in the URT.

A

False

183
Q

TRUE OR FALSE.
N. elongata is an opportunistic pathogen.

A

True

184
Q

A normal oral microbiota in dogs.

A

Neisseria weaveri

185
Q

It is found in humans in infections following dog bites.

A

Neisseria weaveri

186
Q

Neisseria weaveri

Catalase Test Result

A

Positive (+)

187
Q

Neisseria weaveri

Acid Production using COH

A

Negative (-)

188
Q

Neisseria weaveri

Reduction of nitrate

A

Negative (-)

189
Q

Neisseria weaveri

Reduction of nitrite to gas

A

Positive (+)

190
Q

Neisseria weaveri

Phenylalanine deaminase result

A

Weakly Positive (+)

191
Q

Neisseria weaveri is ______ to penicillin.

A

sensitive

192
Q

Neisseria weaveri is sensitive to ______.

A

penicillin

193
Q

Moraxella spp. belong to the family ______.

A

Moraxellaceae

194
Q

Three genera under Family Moraxellaceae

A
  1. Moraxella spp.
  2. Acinetobacter spp.
  3. Psychrobacter spp.
195
Q

It is isolated only from humans.

A

Moraxella catarrhalis

196
Q

TRUE OR FALSE.
M. catarrhalis is a commensal of the GIT.

A

False

Correct ans: URT

197
Q

The third most common cause of acute otitis media and sinusitis.

A

Moraxella catarrhalis

198
Q

M. catarrhalis is the________ cause of acute otitis media and sinusitis.

A

third most common

199
Q

M. catarrhalis is the third most common cause of ________.

A

acute otitis media and sinusitis

200
Q

Moraxella catarrhalis

Oxidase Test Result

A

Positive (+)

201
Q

Moraxella catarrhalis

Catalase Test Result

A

Positive (+)

202
Q

Can Moraxella catarrhalis utilize carbohydrates?

A

No, it is Asaccharolytic

203
Q

Moraxella catarrhalis

Antimicrobial Agents

A
  1. Amoxicillin-clavulanic acid
  2. Extended-spectrum cephalosporins
  3. Azithromycin
  4. Quinolones
  5. Trimethoprim-sulfamethoxazole
204
Q

Which tests can be used to differentiate Moraxella catarrhalis from Neisseria spp.?

A
  1. DNAse
  2. Butyrate esterase

M. catarrhalis is positive (+) for both, while Neisseria spp. is (-)

205
Q

Moraxella catarrhalis

Specimen Collection

A

Middle ear effusions, nasopharynx, sinus aspirates, etc.

206
Q

Moraxella catarrhalis

Culture and Incubation

A
  • SBA/BAP and CHOC/CAP Agar
  • Can tolerate lower temperatures and grow well at 28 C
207
Q

Moraxella catarrhalis grow well at ____ C.

A

28 C

208
Q

Moraxella catarrhalis

Colonial Appearance

A
  • Smooth, opaque, gray to white colonies
  • Hockey puck appearance
  • Wagon-wheel appearance (older colonies)
209
Q

Why are M. catarrhalis colonies called “hockey puck”?

A

Because it remains intact when pushed accross the media with a loop (glides)

210
Q

Growth on Modified Thayer Agar

Moraxella catarrhalis

A

Variable

211
Q

Growth on Modified Thayer Agar

Neisseria cinerea

A

Variable

212
Q

Growth on Modified Thayer Agar

Neisseria elongata

A

Variable

213
Q

Growth on Modified Thayer Agar

Neisseria flavescens

A

Negative (-)

214
Q

Growth on Modified Thayer Agar

Neisseria gonorrhoeae

A

Positive (+)

215
Q

Growth on Modified Thayer Agar

Neisseria lactamica

A

Positive (+)

216
Q

Growth on Modified Thayer Agar

Neisseria meningitidis

A

Positive (+)

217
Q

Growth on Modified Thayer Agar

Neisseria mucosa

A

Negative (-)

218
Q

Growth on Modified Thayer Agar

Neisseria polysaccharea

A

Negative (-)

219
Q

Growth on Modified Thayer Agar

Neisseria sicca

A

Negative (-)

220
Q

Growth on Modified Thayer Agar

Neisseria subflava

A

Negative (-)

221
Q

Growth on Nutrient Agar at 35 C

Neisseria lactamica

A

Variable

222
Q

Growth on Nutrient Agar at 35 C

Neisseria gonorrhoeae

A

Negative (-)

223
Q

Growth on Nutrient Agar at 35 C

Neisseria meningitidis

A

Negative (-)

224
Q

Growth on Nutrient Agar at 35 C

Neisseria polysaccharea

A

Negative (-)

225
Q

Growth on Nutrient Agar at 35 C

Moraxella catarrhalis

A

Positive (+)

226
Q

Growth on Nutrient Agar at 35 C

Neisseria cinerea

A

Positive (+)

227
Q

Growth on Nutrient Agar at 35 C

Neisseria elongata

A

Positive (+)

228
Q

Growth on Nutrient Agar at 35 C

Neisseria flavescens

A

Positive (+)

229
Q

Growth on Nutrient Agar at 35 C

Neisseria mucosa

A

Positive (+)

230
Q

Growth on Nutrient Agar at 35 C

Neisseria sicca

A

Positive (+)

231
Q

Growth on Nutrient Agar at 35 C

Neisseria subflava

A

Positive (+)

232
Q

Growth on Blood or Chocolate Agar at 25 C

Moraxella catarrhalis

A

Positive (+)

233
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria cinerea

A

Negative (-)

234
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria elongata

A

Positive (+)

235
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria flavescens

A

Positive (+)

236
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria gonorrhoeae

A

Negative (-)

237
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria lactamica

A

Variable

238
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria meningitidis

A

Negative (-)

239
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria mucosa

A

Positive (+)

240
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria polysaccharea

A

Negative (-)

241
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria sicca

A

Positive (+)

242
Q

Growth on Blood or Chocolate Agar at 25 C

Neisseria subflava

A

Positive (+)

243
Q

Rapid Fermentation Sugars: GLUCOSE

Moraxella catarrhalis

A

Negative (-)

244
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria cinerea

A

Negative (-)

245
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria elongata

A

Negative (-)

246
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria flavescens

A

Negative (-)

247
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria gonorrhoeae

A

Positive (+)

248
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria lactamica

A

Positive (+)

249
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria meningitidis

A

Positive (+)

250
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria mucosa

A

Positive (+)

251
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria polysaccharea

A

Positive (+)

252
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria sicca

A

Positive (+)

253
Q

Rapid Fermentation Sugars: GLUCOSE

Neisseria subflava

A

Variable

254
Q

Rapid Fermentation Sugars: MALTOSE

Moraxella catarrhalis

A

Negative (-)

255
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria cinerea

A

Negative (-)

256
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria elongata

A

Negative (-)

257
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria flavescens

A

Negative (-)

258
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria gonorrhoeae

A

Negative (-)

259
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria lactamica

A

Positive (+)

260
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria meningitidis

A

Positive (+)

261
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria mucosa

A

Positive (+)

262
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria polysaccharea

A

Positive (+)

263
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria sicca

A

Positive (+)

264
Q

Rapid Fermentation Sugars: MALTOSE

Neisseria subflava

A

Positive (+)

265
Q

ALL are negative for Lactose EXCEPT ______.

A

Neisseria lactamica

266
Q

ALL are negative for Sucrose EXCEPT _________.

A

Neisseria mucosa and Neisseria sicca

267
Q

ALL are negative for Fructose EXCEPT _________.

A

Neisseria mucosa and Neisseria sicca

268
Q

Nitrate Reduction

ALL are negative EXCEPT _________.

A

Moraxella catarrhalis
Neisseria mucosa

269
Q

Gas from Nitrate Reduction

ALL are negative EXCEPT

A

Neisseria mucosa

270
Q

0.01% Nitrate Reduction

Moraxella catarrhalis

A

Variable

271
Q

0.01% Nitrate Reduction

Neisseria cinerea

A

Positive (+)

272
Q

0.01% Nitrate Reduction

Neisseria elongata

A

Negative (-)

273
Q

0.01% Nitrate Reduction

Neisseria flavescens

A

Positive (+)

274
Q

0.01% Nitrate Reduction

Neisseria gonorrhoeae

A

Negative (-)

275
Q

0.01% Nitrate Reduction

Neisseria lactamica

A

Positive (+)

276
Q

0.01% Nitrate Reduction

Neisseria meningitidis

A

variable

277
Q

0.01% Nitrate Reduction

Neisseria mucosa

A

Positive (+)

278
Q

0.01% Nitrate Reduction

Neisseria polysaccharea

A

Negative (-)

279
Q

0.01% Nitrate Reduction

Neisseria sicca

A

Positive (+)

280
Q

0.01% Nitrate Reduction

Neisseria subflava

A

Positive (+)

281
Q

Organism that is negative for Superoxol (30% H2O2)

A

Neisseria elongata