ParaLab - Diagnostic Para Flashcards
(53 cards)
simplest and easiest technique to facilitate detection of intestinal parasites that infected subjects pass in their feces
direct fecal smear
used to directly detect presence of protozoans and helminths?
DFS
what solution in the DFS is used for the detection of trophozoite and larva motility?
NSS
Normal Saline Solution
- may also detect RBCs, Leukocytes, Charcot-Leydens
What solution do u use in DFS to demonstrate parasite structure?
Lugol’s Iodine
Iodine destroys trophozoites and organisms present
What solution do you use when u want to detect protozoan cysts?
Lugol’s
How many drops each should you use in NSS & Lugol’s?
1 drop each
NSS muna b4 Lugol’s coz iodine is toxic sa parasites
this is the qualitative analysis ng laboratory diagnosis since parasite identification is employed. what test is it?
DFS
This is the quantitative part ng Lab Diagnosis tests. It is used to count eggs of STHs.
Kato-Katz Technique
what is egg-counting used for?
- assess efficacy of treatment
- correlate severity of disease w/ intensity of infection
this is the manner of reporting when using Kato-Katz technique
epg
eggs per gram stool
what are the STHs?
- H = ookworm
- A = scaris lumbricoides
- T = richuris trichiura
- S = trongyloides
Schistosoma is not an STH pero nabibilang siya sa katokatz; Strongy no to kato afaik
for how many hours should you soak cellophane in the clearing agent first?
for 24 hours
if cellophane isn’t color green, add malachite green
where do you soak the green-colored cellophane?
what’s the clearing agent used?
glycerol-glycerine
why do we use green cellophane or malachite green?
other colors are strenuous. para masarap sa mata
steps in Kato-Katz
no need to memo this. just understand
- sieve stool using nylon screen
- scrape stool
- prep slide and template w/ hole then pahid stool then meron na sa slide
- place now the soaked cellophane then flatten by flipping or wine cork
- set aside for 1 hour so glycerol (clearing agent) may do its part
In Kato-Katz, hookworm eggs clear rapidly and won’t be visible after ____ to ____ mins
30 to 60 mins
so u need to leave slide w/ cellophane for that long lang
Schistosome eggs in Kato-Katz procedure is preferable examined within how many hours?
24 hours
True or False
Ascaris lumbricoides and Trichuris trichiura eggs will still be visible after many months in DFS
False, in Kato-Katz
for a 9 x 1 mm of template, how many grams of stool will u get and how much will u multiply that to get the epg?
50mg; x20
for a 6 x 1.5 mm of template, how many grams of stool will u get and how much will u multiply that to get the epg?
41.7 mg; x24
for a 6.5 x 0.5 mm of template, how many grams of stool will u get and how much will u multiply that to get the epg?
20mg; x50
pansin niyo, mg is indirectly proportional to amt multiplied bababhawdbaa xori i miss p6
Hookworm classification of intensity of infection for Kato-Katz
light< 2k-4k >=high
lesser than 2k is light, moderate is gitna. greater than or equal to 4k is high
Ascaris lumbricoides classification of intensity of Infection for Kato-Katz
light< 5k-50k >=heavy
lesser than 5k is light; between is moderate; greater than or equal to 50k is high
Trichuris trichiura classification of intensity of Infection for Kato-Katz
light< 1k-10k >= heavy
lesser than 1k is light and gr8r than or = to 10k is heavy
