1S [LEC]: Enzymes (AST, ALT, ALP, ACP)

Question 1

AST & ALT measurement uses ___ as coenzyme

Answer 1

Pyridoxal phosphate

Question 2

Decreased AST occurs in the case of ___

Answer 2

Uremia

Question 3

An increase in both AST & ALT can be a sign of what condition?

Answer 3

Viral hepatitis

Question 4

A four-fold increase in AST may be a sign of what condition?

Answer 4

Cirrhosis

Question 5

AST isoenzyme that is predominant in the serum

Answer 5

Cell cytoplasm AST

Question 6

AST isoenzyme predominant during necrosis

Answer 6

Mitochondrial AST

Question 7

Reference range of AST

Answer 7

5-35 U/L

Question 8

Onset of CK-MB

Answer 8

4-8 hrs

Question 9

Peak of CK-MB

Answer 9

12-24 hrs

Question 10

Duration of elevation of CK-MB

Answer 10

2-4 days

Question 11

Onset of AST

Answer 11

8-12 hrs

Question 12

Peak of AST

Answer 12

24 hrs

Question 13

Duration of elevation of AST

Answer 13

5 days

Question 14

Onset of LD

Answer 14

12-24 hrs

Question 15

Peak of LD

Answer 15

72 hrs

Question 16

Duration of onset of LD

Answer 16

10 days

Question 17

A more specific enzyme to the liver that catalyzed the transfer of an amino group to a-ketoglutarate

Answer 17

Alanine aminotransferase

Question 18

Tissue sources of ALT

Answer 18

Liver, cardiac tissue

Question 19

Enzyme affected in infectious mononucleosis

Answer 19

ALT

Question 20

T/F: ALT is relatively unaffected by hemolysis

Answer 20

True

Question 21

Reference range of ALT

Answer 21

7-45 U/L

Question 22

Activators required in ALP

Answer 22

Mg2+, Mn2+

Question 23

Optimal pH of ALP

Answer 23

9-10

Question 24

Major tissue sources of ALP

Answer 24

Liver
Bone
Placenta
Intestine

Question 25

Carcino-placental ALP that is highest in ovarian and gynecologic cancers, lung, and breast

Answer 25

Regan ALP

Question 26

Carcino-placental ALP that is used for monitoring treatment because it disappears upon successful therapy

Answer 26

Regan ALP

Question 27

Carcino-placental ALP that is a variant of Regan ALP

Answer 27

Nagao ALP

Question 28

Carcino-placental ALP that is detected in metastatic carcinoma of pleural surfaces and adenocarcinoma of the pancreas and bile duct

Answer 28

Nagao ALP

Question 29

Most anodal ALP isoenzyme

Answer 29

Liver

Question 30

Most heat stable ALP isoenzyme

Answer 30

Placental

Question 31

Placental ALP, Regan, and Nagao are the most heat stable and resist heat denaturation at ___ for ___ mins

Answer 31

65C for 30 mins

Question 32

Conditions of the heat stability test for ALP isoenzymes

Answer 32

56C for 10 mins

Question 33

If phenylalanine is added to the ALP isoenzyme and 75% is inhibited, the ALP isoenzyme is identified as ___

Answer 33

Intestinal

Question 34

If phenylalanine is added to the ALP isoenzyme and 80% is inhibited, the ALP isoenzyme is identified as ___

Answer 34

Placental

Question 35

If L-leucine is added to the ALP isoenzyme and is inhibited, the ALP isoenzyme is identified as ___

Answer 35

Nagao

Question 36

If 2M urea is added to the ALP isoenzyme and 90% is inhibited, the ALP isoenzyme is identified as ___

Answer 36

Bone

Question 37

If 2M urea is added to the ALP isoenzyme and 60% is inhibited, the ALP isoenzyme is identified as ___

Answer 37

Liver

Question 38

Identify the method of ALP determination based on the substrate: B-glycerophosphate or Na-glyverophosphate

Answer 38

Bodansky

Question 39

Identify the method of ALP determination based on the substrate: Phenylphosphate or disodium phenylphosphate

Answer 39

King-Armstrong or Gutman-Gutman

Question 40

Identify the method of ALP determination based on the substrate: p-nitrophenylphosphate

Answer 40

Hudson Bowers-McComb

Question 41

Identify the method of ALP determination based on the substrate: p-nitrophenylphosphate (brown)

Answer 41

Bessery-Lowry-Brock

Question 42

Identify the method of ALP determination based on the substrate: a-naphthylphosphate

Answer 42

Babson and Reed

Question 43

Identify the method of ALP determination based on the substrate: Thymolphthalein monophosphate

Answer 43

Roy

Question 44

Identify the method of ALP determination based on the substrate: B-glycerophosphate

Answer 44

Sinowara-Jones-Reinhart

Question 45

Optimal pH for ACP

Answer 45

5.0-6.0

Question 46

Enzyme measured for platelet damage

Answer 46

ACP

Question 47

Reagent that inhibits prostatic ACP

Answer 47

Tartrate

Question 48

Reagent that inhibits non-prostatic ACP

Answer 48

Copper

Question 49

For ACP testing, if the sample is not assayed immediately, the serum must be frozen of acidified to what pH level for it to be stable for 2 days?

Answer 49

6.5