1S [LEC]: Liver function pt. 2

Question 1

In Muelengracht Method of determining icterus index, the serum is diluted with ___

Answer 1

NSS

Question 2

In Muelengracht Method of determining icterus index, the diluted fluid is compared with ___

Answer 2

0.01% K2Cr2O7

Question 3

In Newberger Method of determining icterus index, the serum is diluted with ___

Answer 3

Sodium citrate

Question 4

Specimen of choice of the classic diazo reaction of bilirubin determination

Answer 4

First morning urine

Question 5

In the Evelyn and Malloy method of bilirubin determination, which reagent acts as a buffer?

Answer 5

Sodium acetate

Question 6

In the Evelyn and Malloy method of bilirubin determination, which reagent terminates the reaction by destroying the diazo reagent?

Answer 6

Ascorbic acid

Question 7

Composition of Diazo rgt

Answer 7

Sulfanilic acid
HCl
Sodium nitrite

Question 8

T/F: Jendrassik & Grof is more sensitive than Evelyn & Malloy

Answer 8

True

Question 9

Accelerator of Evelyn & Malloy bilirubin determination method

Answer 9

50% Methanol

Question 10

End color of Evelyn & Malloy bilirubin determination method

Answer 10

Pink to purple

Question 11

Accelerator of Jendrassik & Grof bilirubin determination method

Answer 11

Caffeine Sodium Benzoate

Question 12

End color of Jendrassik & Grof bilirubin determination method

Answer 12

Blue

Question 13

One of the advantages of Jendrassik & Grof bilirubin determination method is that it is not affected by hemoglobin up to ___

Answer 13

750 mg/dL

Question 14

Component of the Ehrlich’s reagent

Answer 14

p-dimethyl aminobenzaldehyde

Question 15

Reagent in the Ehrlich’s method that stops the reaction and minimizes the combination of other chromogens

Answer 15

NaCH3COO

Question 16

Product of Ehrlich’s method of urine urobilinogen determination

Answer 16

Red colored compound

Question 17

Reducing agent in the Ehrlich’s method of urine urobilinogen that maintains urobilinogen in reduced state

Answer 17

Ascorbic acid

Question 18

Specimen of choice in Ehrlich’s method of urine urobilinogen determination

Answer 18

2 hour-fresh urine

Question 19

The bilirubin will decay by how many percent when exposed to light?

Answer 19

30-50%

Question 20

Conversion factor for bilirubin determination

Answer 20

17.1

Question 21

This reagent converts back urobilin to urobilinogen

Answer 21

Alkaline ferric hydroxide

Question 22

Fecal urobilinogen is measured through what method/s?

Answer 22

Ehrlich’s aldehyde
Watsons’ Reaction

Question 23

In the Hay test of bile salt determination, what reagent is sprinkled to 5mL of urine?

Answer 23

Sulfur powder

Question 24

In the Hay test of bile salt determination, if the sulfur powder floats at the top, the result is ___

Answer 24

Negative

Question 25

In the Hay test of bile salt determination, if the sulfur powder sinks at the bottom, the result is ___

Answer 25

Positive (obstructive jaundice)

Question 26

Radioisotope used in the Rose Bengal Test of excretory function

Answer 26

Iodine 131

Question 27

Methods used in testing for excretory function

Answer 27

Bromsulphalein test Rose Bengal test

Question 28

In the Quick's test or Hippuric Acid test, the patient is given a ___

Answer 28

Sodium benzoate meal