2.4 Catalase Enzyme Experiment Flashcards Preview

A Level Biology > 2.4 Catalase Enzyme Experiment > Flashcards

Flashcards in 2.4 Catalase Enzyme Experiment Deck (15):
1

What is the IV in this experiment?

Temperature

2

What is the DV in this experiment?

Volume of O2

3

What are other variables that need to be controlled?

PH
Substrate concentration
Enzyme concentration

4

1. Set up boiling tubes with same volume and concentration of what?

Hydrogen peroxide

5

1. How do you keep PH constant, what does this do?

Adding buffer solution
Resists changes in PH when small amounts of acids and alkalis are added

6

2. What apparatus is set up to measure volume of O2 production?

Delivery tube and upside down measuring cylinder

7

3. Each boiling tube containing hydrogen peroxide is put where?

Water bath

8

3. What is different about each water bath?

Each set to different temperature

9

3. What else is put in a water bath other than hydrogen peroxide solution?

Tube containing catalase

10

3. How long do you wait for each enzyme to reach set temperature before removing from water bath?

5 minutes

11

4. Then use a pipette to add what?

The same volume and concentration of catalase to each tube

12

5. The record O2 production in the ...

First minute reaction

13

6. How many times do you repeat the experiment?

3 times at each temperature

14

6. What do you then use the results to calculate?

Mean O2 production at each temperature

15

7. You can then calculate mean rate of reaction at each temperature, what is the calculation for this?

Volume of O2 produced ÷ time taken (cm3s-1)

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