Biochem: molecular/cellular bio, genetics Flashcards Preview

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Flashcards in Biochem: molecular/cellular bio, genetics Deck (73)
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1

uracil is made from deamination of

cytosine

2

cytosine deamination yields

uracil

3

leflunomide MOA

blocks dihydroorotate dehydrogenase to prevent conversion of carbamoyl phosphate to orotic acid

4

hydroxyurea MOA

inhibits ribonucleotide reductase to prevent conversion of UDP to dUDP

5

substances from glutamine + CO2 to synthesis of CTP

carbamoyl phosphate, orotic acid, UMP, UDP, CTP

6

substances from glutamine + CO2 to synthesis of dTMP

carbamoyl phosphate, orotic acid, UMP, UDP, dUDP, dUMP, dTMP

7

MTX, TMP, and pyrimethamine block

dihydrofolate reductase, which normally recycles DHF into THF

8

5FU blocks

thymidylate synthase, which normally converts dUMP to dTMP
Note: the active form of 5FU is 5FdUMP

9

azathioprine/6MP MOA

impairs purine synthesis by blocking formation of IMP from PRPP

10

Mycophenolate and ribavirin MOA

blocks IMP dehydrogenase to prevent conversion of IMP to GMP

11

probenecid MOA

stimulates conversionof uric acid to urine

12

Lesch-Nyhan (deficiency, symptoms)

HGPRT: hyperuicemia, gout, pissed off, retardation, dystonia

13

this sugar unbinds the repressor on lac operon

high lactose

14

XP is caused by a defect in

NER, which removes oligonucleotides e.g. pyrimidine dimers

15

Spontaneous deamination is repaired by

BER. AP endonuclease cleaves 5' end, lyase cleaves 3' end

16

Ataxia telangiectasia and Fanconi anemia have a defect in this DNA repair mechanism

NHEJ

17

function of eukaryotic RNA pol I

rRNA

18

function of eukaryotic RNA pol II

mRNA

19

function of eukaryotic RNA pol III

tRNA

20

what are the functions of the T arm and D arm of tRNA?

ribosome binding; aminoacyl-tRNA synthase recognition

21

Defect in I-cell disease

failure to add mannose-6-P (defect in n-acetylglucosaminyl-1-phosphotransferase)

22

Sx of I-cell disease

coarse facies, clouded corneas, restricted joint movement, high plasma level of lysosomal enzymes

23

this protein is involved in retrograde vesicular trafficking

COP I

24

this protein is involved in anterograde vesicular trafficking

COP II

25

this protein is involved in transport between Golgi and lysosomes, and in receptor mediated endocytosis

Clathrin

26

stain for mesenchymal intermediate filaments

vimentin

27

this microtuble motor protein does anterograde transport (toward positive end)

kinesin

28

this microtubule motor protein does retrograde transport (toward negative end)

dynein

29

ouabin MOA

inhibits Na/K ATPase by binding to K+ site

30

classical Ehlers-Danlos is a deficiency in this collagen

type V