Exam 2: Sperm collection Flashcards

(45 cards)

1
Q

what are the two ways we can collect sperm

A

mounting a sow/gilt in estrus (rare)

mounting a dummy sow (more common)

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2
Q

How is sperm collected using the mounting a sow/gilt in the estrus method

A

boar mount gilt/sow

penis is diverted from female to collection vesicle

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3
Q

collecting using the mounting a dummy sow

A

preferred

boar is trained to mount dummy

most boar studs have separate collection area and pen

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4
Q

problems with using mounting a sow/gilt in the estrus method

A

not as hygienic

difficult to do

boar may be too big for female

sows may walk around pen

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5
Q

pros of using the mounting a dummy sow method

A

female not required
adjustable height
good footing around the dummy makes it easier for boar
cleaner
can be cleaned
saliva from previous boar can help with arousal

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6
Q

after the boar has mounted the dummy, what needs cleaned

A

preputial opening and surrounding areas need cleaned with disposable wipe

hair may need to be trimmed

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7
Q

after the boar has mounted the dummy, what needs to be cleaned

A

preputial opening and surrounding areas need cleaned with disposable wipe

hair may need to be trimmed

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8
Q

why does the collector wear 2 pairs of gloves

A

the collector will massage the penis through the prepuce and evacuate preputial fluids in the prepuce

once boar extends his penis out of his sheath and thrust, the 1st pair comes off

then with the second pair of gloves on, the collector will grab the penis and apply pressure

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9
Q

the first few jets of ejaculate function to

A

flush out the urethra

should be allowed to go to the ground

clear, watery containing NO SPERM and HIGH BACTERIA

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10
Q

what is a common collection vesicle used in sperm collection

A

pre-warmed (100F) insulated thermos or styrofoam cup with a filter

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11
Q

should the gel-like fraction be filtered out of ejaculated

A

YES

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12
Q

a minimum of _____________ minutes is necessary for a boar to complete ejaculation with the average being ___________ minutes

A

5-7
7-15

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13
Q

Ejaculate volume can vary depending on

A

boar’s age
boars size
collection technique
frequency of technique

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14
Q

the complete process of collection

A
  1. boar investigates and mounts dummy
  2. prepuce cleaned
  3. massage prepuce
  4. remove the outer glove
  5. collector applies pressure to the corkscrew portion of the penis once extended from the sheath (uniform pressure, hold perpendicular)
  6. first part of ejaculate is discarded
  7. once thrusting stops, start collection
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15
Q

T/F latex kills sperm

A

TRUE

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16
Q

after sperm collection is complete, what is done

A

it is sent to the lab for evaluation and processing

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17
Q

high quality boar semen is essential for obtaining

A

satisfactory fertility rates

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18
Q

standard test uses to evaluate boar semen quality include

A

motility
morphology
concentration

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19
Q

normal semen appearance/consistency

A

milky, creamy consistancy

20
Q

normal semen color

A

grey-white to white in color

**yellow, red= infection

21
Q

normal semen total volume

22
Q

normal semen total sperm numbers

A

60-130 billion

**young= ~40 billion
**# drop if sick

23
Q

normal semen: motile sperm

24
Q

normal semen: cytoplasmic droplets

A

less than 15%

25
what is a cytoplasmic droplet and what causes it
extra fluid at tail and head caused by injury, heat stress, general stress
26
examples of sperm morphology problems
short detached coiled tail bent tail
27
factors that influence sperm quality
breeds/lines testicle size and weight age sperm abnormalities social season temp
28
how does temp affect sperm quality
14 and lower impacts fertility because too much body heat is used to stay warm 85 or higher reduces production and motility causing higher protein in semen and reduced shelf life
29
how does season affect sperm quality
highest production: September - February lowest production: march - august
30
what is the most important factor in terms of sperm development
temperature
31
how does social affect sperm quality
physical isolation lowers ejaculate quantity
32
how does sperm abnormalities affect sperm quality?
takes 14 days to appear in ejaculate formed during time of stress or injury to travel to epididymis length of appearance in ejaculate depends on severity and length of stress cytoplasmic droplets frequently seen in boars experiencing heat stress
33
how does age affect sperm quality
boars under 1yr: collect once a week boars over a 1yr: collect 2x week (can decrease morph and fertility) old boars (6+): more sperm abnormalities lower conception rates smaller litters
34
how does testicle size and weight affect sperm quality
weight: correlated to sperm output correlated to the level of testosterone produced size (length and width): related to the age of puberty in male and female offspring no relation to sperm production or fertility
35
how does breed/lines affect sperm quality
meishan: more motile sperm earlier than U.S and europen breeds maternal= normally more sperm
36
3 main ways of semen processing
extended (most common) pooled semen from a particular boar
37
why is extended semen used most often
can be used up to 10 days post collection greatest farrowing rate and litter size in achieved within 24-48 hours after collection increases shelf life increases number of doses
38
what do extender packages have in them
normally a powder glucose (food for sperm) sodium citrate sodium bicarbonate (buffer) antibiotics (prevent bacterial growth)
39
total number of sperm/dose of extended semen ranges from
2-6 billion 1 dose= 60-120mL
40
how is extended sperm packaged/stored
bags, bottles, tubes avg dose= 65-85ml store in dark place 57-64 degrees gently agitate/rotate at least 2x/ day
41
what is pooled semen
mixing semen from different (similar lines or genetics) boars for processing semen for AI 3-6 ejaculates are pooled used in COMMERCIAL PRODUCTION ONLY
42
why is pooled semen good
3-6 ejaculates increase lab efficiency reduces fertility differences of boars less expensive
43
semen from a particular boar is used in
purebred operations where producers need to know the exact lineage of offspring
44
why dont we normally freeze sperm with pigs
doesnt freeze well bad fertility rates no genetics to fall back on
45
do we use raw semen
NO