Lab 1 Exam - Terminology / Questions Flashcards Preview

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Flashcards in Lab 1 Exam - Terminology / Questions Deck (136)
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1

__________ : Plastic plate in which Agar is placed

Petri plate

2

__________ : Food for the organism

Culture Media

3

__________ : Solid culture media (gel in media)

Agar

4

__________ : is also a generic term used to describe any food for a microbe

Agar

5

__________ : grow on the surface of the agar

Microbes

6

Agar plates are __________ when not in use. and the label goes on the __________

-Upside down
-Backside

7

__________ : Population of microbes growing on the surface of the agar

Colony

8

__________ : Liquid culture media

Broth

9

__________ : Cloudy: broth ________ Indicates microbe are growing in the media

-Turbid
-Turbidity

10

__________ : Destroy all life (microbes)

Sterilize

11

__________ : Free of all life (microbes)

Sterile

12

__________ : A chamber in which steam asunder pressure: used to sterilize material in microbiology

Autoclave

13

__________ : Chamber set at a specific temperature. This is where the inoculated agar plates and tubes containing broth are placed to grow cultures

Incubator

14

__________ : Techniques to avoid contamination

Aseptic techniques

15

__________ : To introduce or transfer microbes into "something" such as culture media

Inoculate

16

__________ : Ocular Magnification X Objective Magnification

Total Magnification

17

Total Magnification : __________ Magnification X __________ Magnification

-Ocular
-Objective

18

Ocular Magnification is __X

10

19

Objective magnification is the __________

Lens choice

20

__________ : Automatic focusing

Parfocal

21

__________ : The area viewed through the ocular lens

Field of view

22

__________ : The distance between the end of the objective lens and the slide

Working distance

23

__________ : Ability to see detail: Clarity

Resolution

24

__________ : Adjustments in the distance between the ocular lenses to fit the eyes of the user

Interpupillary Adjustment

25

Two ways to control light intensity are:

-On/Off Intensity regulator
-Iris Diaphragm

26

-Focusing Procedure - Shortcut-

1.
2.
3. Adjust the light regulator control to as bright as possible
4. Move low power objective into position
5. Adjust the coarse focus knob so the low power lens is as close as possible to the stage

1. Plug the Microscope in and turn it on
2. Close the iris diaphragm

27

-Focusing Procedure - Shortcut-

1. Plug the Microscope in and turn it on
2. Close the iris diaphragm
3.
4.
5. Adjust the coarse focus knob so the low power lens is as close as possible to the stage

3. Adjust the light regulator control to as bright as possible
4. Move low power objective into position

28

-Focusing Procedure - Shortcut-

1. Plug the Microscope in and turn it on
2. Close the iris diaphragm
3. Adjust the light regulator control to as bright as possible
4. Move low power objective into position
5.

5. Adjust the coarse focus knob so the low power lens is as close as possible to the stage

29

Objective Lens Power:

Scanning Power = __X

4

30

Objective Lens Power:

Low Power = __X

10