Labeling Techniques in Immunoassay Flashcards
a fixed amount of labeled antigen competes with unlabeled antigen from a patient specimen for a limited number of antibody-binding sites
competitive immunoassay
The amount of light emitted is inversely proportional to the amount of analyte (antigen) measured.
competitive immunoassay
In a _________, the sample antigen binds to an antibody fixed onto a solid phase; a second antibody, labeled with a chemiluminescent label, binds to the antigen-antibody complex on the solid phase
sandwich immunoassay
the emitted light is directly proportional to the analyte concentration.
sandwich immunoassay
labels can be divided into five major groups:
(1) luminol;
(2) acridinium esters;
(3) peroxyoxalates;
(4) dioxetanes;
(5) tris(2,2′−bipyridyl)-ruthenium (II).
___________ (from the bioluminescent jellyfish, Aequorea) activated by reaction with is another label
native or recombinant apoaequorin ; coelenterazine
EMERGING LABELING TECHNOLOGIES
1 Fluorescence in situ hybridization (FISH)
2 Solid-Phase Immunosorbent Assay (SPIA)
FISH uses ________ to brightly “paint” genes or chromosomes
fluorescent molecules
FISH uses ______ technology
recombinant DNA
In recombinant DNA technology (FISH)..
______ are short sequences of single-stranded DNA that are complementary to the DNA sequences to be examined.
Probes
In recombinant DNA technology (FISH)..
Probes hybridize, or bind, to the _______
complementary DNA (cDNA)
In recombinant DNA technology (FISH)..
______ tags indicate the location of the _____
labeled fluorescent ; sequences
the antibody directed toward the agent being assayed is fixed firmly to a solid matrix (either to the inside of the wells of a microdilution tray or to the outside of a spherical plastic or metal bead or some other solid matrix)
Solid-Phase Immunosorbent Assay (SPIA)
