HEMA QUALITY ASSURANCE/CONTROL Flashcards
Specimen Quality Assurance
__________ are not recommended as RBCs contain activating factors for the intrinsic portion of the coagulation cascade…
These may shorten the _____
Hemolyzed specimens ; APTT
Specimen Quality Assurance
Specimen should be centrifuged to get ________ (RCF of ____ for ___ minutes)
platelet - poor plasma ; 1000x g for 10 minutes
Specimen Quality Assurance
_______ are recommended as contact with glass may result in initiation of _________ and the shortening of ____
Plastic tubes ; coagulation cascade ; APTT
Specimen Quality Assurance
The plasma should be processed within ______ or stored at _____ in a tightly stoppered tube
30 min ; 4°C
_____________ is set up within a laboratory to monitor and ensure the reliability of test results from that laboratory
Internal Quality Control
The primary tool for internal quality control is called a ________.
control
A control is a specimen with a predetermined range of result values, called ________, that is processed in the same manner as a patient sample.
control values
In Internal Quality Control, ________ are processed with each series or run of patient samples.
control samples
In Internal Quality Control, If the result of a test on a control sample is different from its known value, this indicates a problem in the _________ being used.
equipment/methods
Internal Quality Control in Hematology Lab
The purpose of running IQA is to:
- Check the accuracy of tests system by comparing the daily run to the assigned Mean Value.
- Assess the precision of test system through cumulative run comparison CV%
- Predict and or to detect the potential errors and fluctuations in test system before it could affect the Patients Results.
- Assure the stability of analytical runs by running control every 8 hours.
Is an important component of the total quality assurance program of a clinical hemostasis lab such as PT, APTT, coagulation factor activity assays (e.g., fibrinogen, factor [F] VIII, FIX, FXI), vWf assays, unfractionated heparin monitoring, lupus anticoagulant testing, and plt function.
EXTERNAL QUALITY ASSURANCE (EQA)
MAJOR BENEFITS OF EQA
1) Enhance patient care and safety through improved laboratory testing;
2) characterize test accuracy and precision across multiple methods;
3) correlate specific method variables with accuracy and precision;
4) identify interfering substances and quantify their effects across multiple methods;
5) identify clinical laboratories that are at risk for poor performance so that their performance can improve; 6) satisfy accreditation and regulatory requirements.
These are variables that can occur from the time
when the test is ordered by the physician until
the sample is ready for analysis.
pre-analytical variables
Effects of Pre-analytical Variables on the Quality
of Laboratory Testing
The ________ is the most important.
pre-analytical stage
Pre-analytical variables account for up to _____ of
laboratory errors.
75%
