Lecture 11 - Enzymes I Flashcards
The most common of all enzymatic reactions.
Redox (oxidation-reduction) reactions.
Primary f(x)s of enzymes
Lower activation energy (Ea) and stabilize the transition state)
How does one increase the production of products (according to Le Chatlier’s Principle)?
Decrease [product] or increase [reactants]
What is meant by “coupled reactions” in terms of biochemical reactions?
The energies of a set of reactions are additive. In other words, if A-> B has a (+) G, and B->D has a (-) G, they can be added together. If the sum of both G’s is (-), the reaction is OVERALL spontaneous.
These reactions revolve around the transfer of electrons.
Gain of an e-
loss of e-
Gain of H+
Loss of H+
What portion of an enzyme determines specificity for a substrate?
Cleavage of peptide bonds
What is the Vmax?
The maximum rate (reaction velocity) at which a reaction can occur.
What produces a maximum velocity (Vmax) in reactions?
The complete saturation of the active site on an enzyme.
3-D region or crevice that is produced after the residues from various parts of a protein come together in the 3* structure