Lecture 2/3-properties Of Biological Molecules I&II Flashcards Preview

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Flashcards in Lecture 2/3-properties Of Biological Molecules I&II Deck (32):
1

Standard free-E* change equation

Delta G = -(R)(T)ln(Keq)

2

Gibbs Free E* equation

Delta G=Delta G*'+ln([Prod]/[React])

3

Three types of non-covalent bonds

Hydrophobic interactions, van der Waal's, electrostatic interactions

4

What produces the "hydrophobic effect?"

Water has a dipole moment, which results in a slight (+) and (-) charge on water. This allows for hydrogen bonding

5

How can one drive a reaction to the product side? What is this manipulation called?

Add reactant/remove products - Le Chatlier's Principle ("Law of Mass Action"); also, add heat

6

The reaction of Glucose to Glucose-6-phosphate occurs by the enzyme hexokinase. What is the Delta-G prime for this reaction if there is 1 mole of glucose, and 1000 moles of G-6-P?

Delta G' = -RT(ln[prod]/[react]) = -8.31(310)(ln[1000]/[1]]) = -2576.1xln([1000]/[1]]) = -17.8kJ/mol

7

The reaction of Fructose-6-P to Fructose-1,6-bisphosphate occurs by the enzyme phosphofructokinase. What is the Delta-G prime for this reaction if there is 100 mole of F-6-P, and 1 mole of F-1,6-BP?

Delta G' = -RT(ln[prod]/[react]) = -8.31(310)ln([100]/[1]) = -11863.4 Jmol^-1/1000 = -11.9 kJ/mol

8

The reaction of Fructose-6-P to Fructose-1,6-bisphosphate occurs by the enzyme phosphofructokinase. What is the Delta-G prime for this reaction if there is 10 mole of F-6-P, and 1 mole of F-1,6-BP?

Delta G' = -RT(ln[prod]/[react])

9

The reaction of Fructose-6-P to Fructose-1,6-bisphosphate occurs by the enzyme phosphofructokinase. What is the Delta-G prime for this reaction if there is 1 mole of F-6-P, and 1000 mole of F-1,6-BP?

Delta G' = -RT(ln[prod]/[react])

10

What is the pH of a solution acetoacetic acid? The [A]=100 and [HA]=1 (pKa=3.58)

pH=pKa+log([A]/[HA] pH=3.58 + log[100]/[1] pH=3.58+2 pH=5.58

11

What is the pH of a solution acetoacetic acid? The [A]=1 and [HA]=1 (pKa=3.58)

pH=pKa+log([A]/[HA] pH=3.58+log([1]/[1]) pH=3.58+0 pH=3.58

12

What is the pH of a solution acetoacetic acid? The [A]=1 and [HA]=100 (pKa=3.58)

pH=pKa+log([A]/[HA] pH=3.58+log([1]/[100]) pH=3.58-2 pH=1.58

13

Energy can neither be created nor destroyed, only changed

First law of thermodynamics

14

The entropy of an isolated system not in equillibrium must increase

Second law of thermodynamics

15

First law of thermodynamics

Energy can neither be created nor destroyed, only changed

16

Second law of thermodynamics

The entropy of an isolated system not in equillibrium must increase

17

Delta G = -(R)(T)ln(Keq)

Standard free-E* change equation

18

Delta G=Delta G*'+ln([Prod]/[React])

Gibbs Free E* equation

19

Hydrophobic interactions, van der Waal's, electrostatic interactions

Three types of non-covalent bonds

20

Water has a dipole moment, which results in a slight (+) and (-) charge on water. This allows for hydrogen bonding

What produces the "hydrophobic effect?"

21

Add reactant/remove products - Le Chatlier's Principle ("Law of Mass Action"); also, add heat

How can one drive a reaction to the product side? What is this manipulation called?

22

Delta G' = -RT(ln[prod]/[react]) = -8.31(310)(ln[1000]/[1]]) = -2576.1xln([1000]/[1]]) = -17.8kJ/mol

The reaction of Glucose to Glucose-6-phosphate occurs by the enzyme hexokinase. What is the Delta-G prime for this reaction if there is 1 mole of glucose, and 1000 moles of G-6-P?

23

Delta G' = -RT(ln[prod]/[react]) = -8.31(310)ln([100]/[1]) = -11863.4 Jmol^-1/1000 = -11.9 kJ/mol

The reaction of Fructose-6-P to Fructose-1,6-bisphosphate occurs by the enzyme phosphofructokinase. What is the Delta-G prime for this reaction if there is 100 mole of F-6-P, and 1 mole of F-1,6-BP?

24

Delta G' = -RT(ln[prod]/[react])

The reaction of Fructose-6-P to Fructose-1,6-bisphosphate occurs by the enzyme phosphofructokinase. What is the Delta-G prime for this reaction if there is 10 mole of F-6-P, and 1 mole of F-1,6-BP?

25

Delta G' = -RT(ln[prod]/[react])

The reaction of Fructose-6-P to Fructose-1,6-bisphosphate occurs by the enzyme phosphofructokinase. What is the Delta-G prime for this reaction if there is 1 mole of F-6-P, and 1000 mole of F-1,6-BP?

26

pH=pKa+log([A]/[HA] pH=3.58 + log[100]/[1] pH=3.58+2 pH=5.58

What is the pH of a solution acetoacetic acid? The [A]=100 and [HA]=1 (pKa=3.58)

27

pH=pKa+log([A]/[HA] pH=3.58+log([1]/[1]) pH=3.58+0 pH=3.58

What is the pH of a solution acetoacetic acid? The [A]=1 and [HA]=1 (pKa=3.58)

28

pH=pKa+log([A]/[HA] pH=3.58+log([1]/[100]) pH=3.58-2 pH=1.58

What is the pH of a solution acetoacetic acid? The [A]=1 and [HA]=100 (pKa=3.58)

29

First law of thermodynamics

Energy can neither be created nor destroyed, only changed

30

Second law of thermodynamics

The entropy of an isolated system not in equillibrium must increase

31

Energy can neither be created nor destroyed, only changed

First law of thermodynamics

32

The entropy of an isolated system not in equillibrium must increase

Second law of thermodynamics