Flashcards in Microscopy Deck (23):
What 3 types of cell exist in single cell form?
Spermatozoa, Ovum & Blood Cells
What is used to preserve tissue and prevent rotting before light microscopy?
What substance is the tissue embedded in to allow very thin slicing in light microscopy? To what typical thickness is the tissue cut?
What stain is commonly used to make the nucleus of cells look clearer and to show more detail in the cytoplasm? What colour does it give?
Haemotoxylin + Eosin (H + E Stain)
What does a haemotoxylin stain do to a cell?
Stains the nucleus most strongly blue
What does Eosin stain do to a cell?
Stains the extracellular matrix and cytoplasm most strongly pink
When taking a frozen section what temperature is the specimen reduced to? What is an advantage and disadvantage of this technique?
-20 to -30 degrees Celsius.
Much faster than traditional techniques.
Lower technical quality of the sections.
What causes gout? What is a common place for gout to be seen?
Too much uric acid. Formation of monosodium urate crystals especially at joints.
What is analgesia?
Rapid pain relief
What types of drugs can be used to treat the pain as a result of gout?
Non-steroidal anti-inflammatory drugs
What type of microscopy can be used to study living cells?
What is the common magnification of a light microscope?
What is the difference between resolvable points when using a light microscope?
Do you get a higher resolution with a shorter or longer wavelength in microscopy? What is the wavelength used in light and TE microscopy?
Light = 400nm
TEM = 1nm
TEM has a 400x greater resolution
What is the typical magnification of a TEM?
Why can't TEM be used to examine living structures?
Electron beam is fired in a VACUUM
How does the electron beam fired in TEM produce an image?
Fired at tissue. Portions that the beam passes through will appear brighter. Portions that absorb/scatter the electrons will appear darker.
What is used to fix a sample in TEM? What is the sample embedded in?
What tool is used to slice samples extremely thinly?
How does freeze fracture EM work?
Tissue frozen to -160 degrees Celsius.
Fractured with a knife - sample interior exposed.
How does SEM work?
Electrons fired at sample, reflected back from the surface of sample and received by cathode ray tube.
What is a cyrostat?
Microtome in a freezer. Used to produce a frozen section.