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Flashcards in Therapeutic viruses Deck (22):
1

therapy modifies gene that my be passed onto subsequent generation

 

germline gene therapy

1

adenovirus replicates where? genome? gene transcription regulated how?

linear dsDNA genome replicates in nucleus with no integration

 

trasncription regulated with immedate early, early, and late classes of mRNA

2

Retrovirus vectors

____integration,

____-term gene expression

potential for _____

replication ____

Retrovirus vectors

stabile integration,

long-term gene expression

potential for insertional mutagenesis

replication incompetent 

3

most populat site for foreign gene in vaccinia vector

TK gene

5

therapy in which genetic modifications restircited to somatic cells with no effect on germline

somatic gene therapy

6

strategy for gene expression with vaccinia vectors

1. plasmid construction with foreign gene + vaccina promoter

7

adenovirus vectors

____integration,

____-term gene expression

immune response to vector ____

replication ____

no integration,

short-term gene expression

vigorous immune response

replication incompetent

 

8

length of adenovirus gene expression

short term (does not integrate)

8

has inherent tumor sleectivity for cells with activated Ras activated, wild type srain with no modifcations by recombinant DNA

Reovirus

9

modifying ___ changes efficient binding to integrins present on many tumor cells to create more efficient binding to targets

RGD (arginaine, glycine, aspartic acid residue)

9

in normal cells, ___ replication is restricted by activation of RNA activated protein kinase PKR

reovirus

10

disadvantages of retrovirus vecotr

random integration into chromosomes > insertional mutagensis

 

maintaining hihg levels of gene expression

 

dpependence on target cell proliferation with MMLV-based vectors

11

hTERT-Ad role

human telomerase reverse transcriptase promoter, iigh active in tumor cells but inactive in normal brain cells, allows for Adenovirus replication

12

Conditionally Replicative Adenovirus, deletion of ____ or ____ regions will allow viral replication only in cells with specific dysfunctions (like p53, RB)

E1A or E1B

13

16

transfer of new genetic material to the cells of an individual to benefit individual

gene therapy

17

other Poxviruses (canarypox, Modified vaccinia ankara) do not multiply in

human cells

18

genome, vaccinia virus

replication location

large, dsDNA

cytoplasmic replication

19

Adenovirus genome CRAd can only replicate where ___ is already mutated (often cancer cells, impacting p53, Bcl-2)

ONYX-15

20

creating adenovirus vector construct

1. delete viral genes to make room for gene of interest

2. transvect vector DNA construct into complementing cell line to produce vector particles

3. particles deliver gene to target cells, does not integrate

21

preintegration complex with Vpr, integrase and matrix proteion will cross nuclear membrane in nondividing cells = ___ vector

lentivirus 

22

Retrovirus vectors retain ___ for integration and ___ for packaging

LTRs for integration and psi sequences for packaging