Retroviruses 2 Flashcards Preview

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Flashcards in Retroviruses 2 Deck (28):
1

CXXR4 role

co receptor for T tropic HIV. binds SDF-1 which can block T tropic infection

2

Vir role

restriction factor, causes cellular antiviral protein to be degraded so that it cannot be incorporated into new virions where it would block RT in the next cell by inducing massive mutations in viral DNA

2

level of CD4 depletion AIDS phase

3

Serology cant detect HIV infection until

4-6 weeks post infection

3

Classes of HIV drugs

RT inhibitor, Protease inhibitor, Fusion inhibitor, Entry inhibitor, integrase inhibitor

4

___ clears viremia during asymptomatic phase, CD4 declines

FDC

5

6 additional accessory proteins required for HIV pathogenesis

Vif, Vpr, Vpu, Nef, Tat, Rev

5

mechanism of direct killing via HIV

massive virus production > membrane leakage AND syncitia of infected cells fuse via Env (infected) CD4/CCR5 (healthy) interactions AND apoptosis induced by infection

6

indirect effects of killing via HIV

CD4 cells >> immune response kills infected cells, soluble gp120 binds uninfected cells, which are then susceptible to ADCC

7

CCR5 role

co receptor for M tropic HIV, receptor for RANTES, MIP-1a, MIP 1B

7

methods of testing

Serology, RNA RT-PCR (virus in blood) Real-time RT-PCR (quantifies virus in blood)

8

RANTES, MIP-1a, MIP-1B role

chemokines specifically inhibit M tropic HIV by occupying CCR5

10

infect primary Tcells and T cell lines, but not macrophages, associated with progression and arise at AIDS stage

T-tropic HIV

12

Dendritic cell role in HIV infection

can be infected, disseminate, but not productively infected

13

homozygous 32bp deletion in ___ gene makes individual incredibly resistant to HIV infection

CCR5

14

macrophage role in HIV infection

infected but not efficiently killed, HIV resevoir

14

after exposure co-receptor binding site via conformational change in env, ___ domains are exposed and enter cell membrane

gp41 fusion domains

15

during asymptomatic phase ___ traps virus, keeping viremia low, but nodes (especially ____) are major sites of replication

FDC traps virus, but GALT major region of replication

17

Tat role

transactivator of transcription (required for transcription)

19

Stromal derived factor 1 role (SDR-1)

cytokine, natural ligand for CXRC4, coreceptor for T tropic

20

Co-receptor engagement triggers ____ of the N and C terminal helical regions of gp41 and fuses them together

snapback

21

initial receptor for HIV

CD4

23

Rev role

regulator of virion expression - allows strucutral gene expression by promoting transport of unspliced RNA to cytoplasm

24

infect primary Tcells and macrophages but NOT Tcell lines, responsible for inititial infection and transmission, predominant in asymptomatic

M tropic

25

Vpu role

promotes virion releasse from cells by inhibiting host tetherin, works on other enveloped viruses

26

in initiation of binding, ___ contacts CD4 and induces a conformtion change in ___ to expose the coreceptor binding site

Env

27

duration of HIV in body

75 years (memory Tcells)

28

Microglia role in HIV

infected > contribute to AIDS dementia