Micro Exam 2 Answers Flashcards
Is rigid with more beta sheets
thermophile
What is the major difference between facultative organisms and aerotolerant anaerobes
facultative organisms grow better w/ oxygen
Has a flexible alpha helix & fewer bonds
Psychrophile
Cross-Linking
Penicillin binding, lose catalytic activity.
Transpeptidation
FtsL
Responsible for rod or bacillus shape of cells
MreB
of Generations=
duration of expo/generation time
unsaturated fatty acids
psychrophile
Can grow in 95oC
hyperthermophiles Archaea domain ONLY
Separation of daughter strands during cell division
FtsK protein
Anchors Z ring to cyto membrane
ATPase
FtsA
Grows at 15oC
psychrophile
has shorter fatty acid chains
psychrophile
Grow at 45oC
thermophile
Forms a Z ring that divides bacterial cells in half
Fts Proteins
the higher the pH
the fewer the H+ ions (more OH) 10times
breaks down H2O2 to oxygen + water
catalase
used to grow a continuous culture in the laboratory
chemostat
Measures cell concentration by turbidity
spectrophotometer
what type of microscope can measure cell counts
phase contrast microscopy
determines cell midpoint during Z ring formation
MIN proteins, middle is most free space
grow best at slightely reduced oxygen levels (3-5% CO2)
Microaerophile
Ten fold Seriel dilution factor
add 10 x each time
cfu/ml=
dilution factor x mL
of cells =
plate count x Dilution Factor/ mL dilution plated
The primase side of template strand
3’ lagging strand
- RNA primer
- Lagging Strand
- Primase
- Single Stranded Binding Protein
- Helicase
- DNA pol III
- Leading Strand
- 5’
Unwinds double helix at replication fork
helicase
lays down RNA at the start of DNA replication
Primase
Seals Okzaki fragments together
ligase
Creates Negative Supercoils in DNA
gyrase
Which direction does sigma recognize promoter sequences
5’ to 3’
The portion of NDA where replication starts is called
origin
Each daugher cell receives a strand of DNA from the parent and newly synthesized complementary strand, what type?
Semi conservative
Proofreading of Replication is performed by
- 3’ to 5’ exonuclease activity of DNA polymerase
- both Pol I & Pol III can do this
Enzyme respobsible for removing RNA primer and replacing with DNA
DNA pol I
Sythesizes leading strand 5’ to 3’
DNA Pol III
What direction does RNA polymerase transcribe
3’ to 5’
Specific DNA sequences that bind RNA polymerase
Promoters