Chapter 4 Flashcards
1
Q
What is DNA’s structure?
A
Long, thread-like molecule w/ uniform diameter and varied length
2
Q
How many DNA molecules in the nucleus of human cells?
A
46 individual, or 23 pairs
3
Q
What type of molecule is DNA?
A
A polymer of nucleotides
4
Q
What are nucleotides made up of?
A
A sugar, phosphate group, and nitrogenous base
5
Q
What are purines?
A
Double-ring nitrogenous bases. Adenine and guanine
6
Q
What are pyrimidines?
A
Single-ring nitrogenous bases. Cytosine, thymine, uracil
7
Q
What are the DNA bases?
A
A, T, C, G
8
Q
How is DNA shaped?
A
Double helix
9
Q
Function of DNA?
A
It codes for the synthesis of RNA and a specific protein
10
Q
What are the DNA base pairs?
A
A-T and C-G
11
Q
What is a chromatin?
A
A fine, filamentous DNA material complexed w/ proteins, located in the cell nucleus
12
Q
What is a histone?
A
Protein core
13
Q
What are nucleosomes?
A
Histones linked together. Chromatin is made of thousands of repeating nucleosomes
14
Q
What is done before cell division?
A
The cell makes a copy of all nuclear DNA. The copies are called sister chromatids
15
Q
Where are chromatids joined at?
A
The centromere
16
Q
Where are kinetochores?
A
The side of each centromere of a chromosome
17
Q
What does RNA do?
A
Carries out the instructions in DNA and assembles proteins
18
Q
How does RNA look?
A
One nucleotide chain (not a double helix)
19
Q
How is RNA structurally different from DNA?
A
It uses ribose instead of deoxyribose as the sugar, and uracil replaces thymine as a nitrogenous base
20
Q
3 types of RNA?
A
Messenger RNA, ribosomal RNA, and transfer RNA
21
Q
What is a gene?
A
An information-containing segment of DNA that codes for the production of a molecule of RNA that plays a role in synthesizing proteins
22
Q
What is a genome?
A
All of the genes of one person
23
Q
What percentage of genes code for DNA?
A
2%, other 98% is noncoding
24
Q
What is genomics?
A
The study of the whole genome and its genes and noncoding DNA interact to affect structure and function of whole organism
25
What is genetic code?
A system that enables four nucleotides to code for amino acid sequences of all proteins. The link between mRNA codons and the 20 amino acids
26
What is a codon?
A 3-base sequence in mRNA
27
What are the stop codons?
UAG, UGA, and UAA
28
What is the start codon?
AUG
29
True or false: any given cell uses 1/3-2/3 of its genes
True
30
What is transcription?
DNA codes for mRNA in nucleus
31
What is translation?
mRNA codes for protein in cytoplasm
32
During transcription, how are base pairs made?
C = G
T -> A
A -> U
33
What is a terminator?
Stop codon, a base sequence at the end of a gene signaling stop
34
What are exons?
Segments of pre-mRNA that will be translated into protein
35
What are introns?
Segments of pre-mRNA that must be removed before translation
36
What are ribosomes?
Organelles that read the tRNA message
37
3 steps of translation?
Initiation, elongation, termination
38
What is initiation?
Small ribosomal unit binds to mRNA in cytosol, tRNA attaches at start codon, then ribosomal unit joins complex
39
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40
What is termination?
When a ribosome reaches a stop codon, a release factor binds to it and the finished protein breaks away from the ribosome.
41
Steps of peptide formation?
1) DNA double helix
2) 7 base triplets on the template strand of DNA
3) Corresponding codons of mRNA transcribed from DNA triplets
4) Anticodons of tRNA that bind to mRNA
5) Amino acids carried by those 6 tRNA molecules
6) Amino acids linked into a peptide chain
42
4 steps of DNA replication?
Unwinding, unzipping, building new DNA strands, repackaging
43
What is the replication fork?
The point of DNA opening
44
What happens during the DNA building new strands?
The polymerase reads exposed bases and matches their pairs, they work on each strand in opposite directions and make a new continuous strand of DNA. 2 molecules are made from 1
45
What is semiconservative replication?
Each daughter DNA consists of one old and one new helix
46
What is a mutation?
Changes in DNA structure due to replication errors or environmental factors (radiation, viruses, chemicals)
47
What can mutations do?
Some have no effect, some kill cells or turn them cancerous, some cause genetic defects in future generations
48
What is the cell cycle?
Cell's life from one division to the next. Interphase and mitotic phase
49
Subphases of interphase?
G1, S, G2
50
Subphases of mitotic phase?
Prophase, metaphase, anaphase, telophase
51
What is G1 phase?
Interval between cell birth and DNA replication. Cell carries out normal tasks and accumulates materials for next phase
52
What is S phase?
Cell replicates all nuclear DNA and duplicates centrioles
53
What is G2 phase?
Interval between DNA replication and cell division. Cell repairs replication errors, grows, synthesizes enzymes that control cell division
54
What is M/mitotic phase?
Cell replicates its nucleus. Pinches in 2 to form new daughter cells
55
What is G0 phase?
Cells that have left the cycle and cease dividing for a while or permanently
56
What is mitosis?
Cell division resulting in 2 genetically identical daughter cells
57
Functions of mitosis?
Develops a person from a fertilized egg. Growth of all tissues and organs after birth. Replace dead cells and repair damaged tissues
58
What happens during prophase?
Genetic material condenses into compact chromosomes. 46 chromosomes total. 2 chromatids per chromosome. Nuclear envelope disintegrates. Centrioles sprout spindle fibers. Some attack to kinetochores of centromeres of chromosomes
59
What happens during metaphase?
Chromosomes align on cell equator. Spindle fibers complete lemon-shaped mitotic spindle. Shorter microtubules from centrioles complete an aster which anchors itself to inside of cell membrane
60
What happens during anaphase?
Enzyme cleaves 2 sister chromatids apart at the centromere. Single-stranded daughter chromosomes migrate to each pole of the cell as motor proteins in kinetochores crawl along spindle fibers
61
What happens during telophase?
Chromosomes cluster on each side of the cell. Rough ER makes new nuclear envelope around each cluster. Chromosomes uncoil to chromatin. Mitotic spindle disintegrates. Each nucleus forms nucleoli.
62
What happens during cytokinesis?
Division of cytoplasm into 2 cells. Telophase overlaps into this. Works bc myosin protein pulls on actin in the cytoskeleton terminal web. Creates cleavage furrow
63
When do cells divide?
- Have enough cytoplasm for 2 daughters
- DNA is replicated
- Enough nutrients
- Stimulated by growth factors (chemicals)
- Neighboring cells die, opening up space
64
When do cells stop dividing?
- Nutrients or growth factors are withdrawn
- Contact inhibition (not enough space)
65
What is heredity?
Transmission of genetic characteristics from parent to offspring
66
How do you inherit chromosomes?
You get 1 chromosome from each parent, for each pair of 23 (so 23 pairs, half mom half dad, 46 chromosomes total)
67
How many autosomes?
22 pairs
68
How many pairs of sex chromosomes?
1
69
What sex is XX chromosomes?
Female
70
What sex is XY chromosomes?
Male
71
What does diploid (2n) mean?
Any cell with 23 pairs of chromosomes (somatic cells)
72
What does haploid (n) mean?
Cells containing 23 unpaired chromosomes. Sperm and egg (germ) cells
73
What does locus mean?
The location of a particular gene on a chromosome
74
What are alleles?
Different forms of a gene at the same locus on 2 homologous chromosomes
75
What are dominant alleles?
Capital letter, makes trait appear, masks recessive allele's effect
76
What are recessive alleles?
Lowercase letter, only seen when both homologous chromosomes are recessive
77
What is a genotype?
Alleles an individual possesses for a particular trait
78
What does homozygous mean?
2 identical alleles for a trait
79
What does heterozygous mean?
2 different alleles for a trait
80
What is a phenotype?
An observable trait
81
What is a gene pool?
Genetic makeup of whole population
82
What is codominance?
Both alleles are equally dominant. Phenotypically expressed
83
What is incomplete dominance?
Heterozygous person shows phenotype intermediate between traits each allele would've produced alone
84
What is polygenic inheritance?
Genes at 2 or more loci contribute to a single phenotypic trait
85
What is pleiotropy?
One gene produces multiple phenotypic effects
86
How do sex-linked traits work?
Carried on X or Y chromosome, and therefore are inherited by one sex more than the other
87
How does colorblindness work?
It's recessive and carried on the X, so it's more common in men
88
What is epigenetics?
A field examining nongenetic changes that alter gene expression and can be passed to offspring
89
What does DNA methylation mean?
It's a mechanism of epigenetic change in which methyl groups are added to DNA, often silencing the gene
90
What does metastasizing mean?
Giving off cells that spread and seed the growth of tumors elsewhere
91
What is oncology?
Medical specialty dealing with tumors
92
What is tumor angiogenesis?
Growth of blood vessels by energy-hungry tumors
93
What are carcinomas?
Cancers of epithelial tissue
94
What are lymphomas?
Cancers of lymph nodes
95
What are melanomas?
Cancer in pigment cells of epidermis (melanocytes)
96
What are leukemias?
Cancer in blood-forming tissues
97
What are sarcomas?
Cancer in bone, other connective tissue, or muscle
98
What are carcinogens?
Environmental cancer-causing agents
99
What is radiation (as a carcinogen)?
Ultraviolet rays, X-rays
100
What are chemicals (as a carcinogen)?
Cigarette tar, food preservatives, industrial chemicals
101
What are viruses (as a carcinogen)?
Human papillomavirus, hepatitis C, and type 2 herpes simplex
102
What do carcinogens do?
Trigger gene mutations
103
How many cancers are hereditary?
5-10%
104
What are oncogenes?
Cause cell division to accelerate out of control. For cancer
105
What are tumor-suppressor (TS) genes?
Healthy tumor suppressor genes inhibit development of cancer, mutated or silenced ones leave oncogene action unopposed
106
Why is cancer lethal?
Replaces functional tissue in vital organs, steals nutrients from rest of body, weakens one's immunity, opens door for opportunistic infections
107
What is cachexia?
Severe wasting away of depleted tissues
