Flashcards in Genetic tests Deck (12)
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1
To see if a mutation is absent or present
Allele specific PCR
2
Determine point mutation
Oligonucleotide ligation assay
3
Copy number changes
MLPA
FISH
qPCR (if FISH unsuitable)
Microarray CGH (genome wide screen)
4
If can't use PCR to detect a known sequence (because strand is too long or GC rich)
Southern Blotting
5
Detect an unknown mutation
DNA sequencing (clonal)
6
Blotting test used for protein
Western
7
Blotting test used for RNA
Northern
8
Blotting test used for DNA
Southern
9
Which patients are tested for a copy number change?
Learning disabilities
Low IQ
Dysmorphic
Autism spectrum
10
To detect prenatal aneuploidy
Quantitative fluorescent PCR
11
Tumours
FISH
12
