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Flashcards in JAALAS 2010 No. 4 KD Deck (53)
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Evans et al.Gray Short-Tailed OpossumWhat is the genus of the gray short-tailed opossum?
a. Didelphis
b. Thylamys
c. Marmosa
d. Monodelphis

D. Monodelphis


The gray short-tailed opossum is a model for what type of hypercholesterolemia?
a. Diet-induced
b. Familial

a. Diet induced


3. Which of the following enzymes is frequently found elevated in young growing mammals?
a. Alanine aminotransferase
b. Aspartate aminotransferase
c. Alkaline phosphatase

c. Alkaline phosphatase


4. In some animals, as in this study, it has been found that hemoglobin has a lower affinity for oxygen in which sex?
a. Males
b. Females

b. Female


5. In this study, the overall body weight and the percentage body weights of the liver was greater in males. T or F



Bauer et alOverweight Body Condition in Laboratory-Housed Cynomolgus Macaques .Most North American facilities housing cynomolgus macaques recognize the importance of tracking body condition T/F



Obesity is not a leading health problem in most facilities housing cynomolgus macaques, with 8/11 facilities reporting <3% of their population overweight.



A common method to decrease excess weight in cynomolgus macaques is calorie restriction, which also has been shown to have beneficial health effects



Body weight tracking and BCS are used to monitor weight status of macaques.



Facilities housing middle-aged macaques (older than 10 years) or long-term maintenance colonies had an increase in overweight animal prevalence.



Takeo et al.Mouse Embryos Produced by Cryopreserved Sperm.1. T / F. The cold storage technique yielded a developmental rate that was greater than 50%.



2. T / F. Fertilization rates of frozen/thawed sperm are generally greater than that of fresh sperm.



Sztein et al. Refinements in the Cryopreservation of Mouse Ovaries.What were the major advantages of the controlled–rate cooling method of cryopreservation of mouse ovaries?
a. Longer preservation times
b. Using the same cooling program for ovarian tissue as that for embryos, and by freezing ovarian tissue in straws rather than vials, both genetic resources can be prepared at the same time.
c. Decreased number of offspring produced after the procedure.
d. Decreased implantation success rates

b. Using the same cooling program for ovarian tissue as that for embryos, and by freezing ovarian tissue in straws rather than vials, both genetic resources can be prepared at the same time


Goulding et al. The Effects of Perioperative Analgesia on Litter Size in Crl:CD1(ICR) Mice Undergoing Embryo Transfer.1. What is the mechanism for analgesia from NSAIDs like flunixin meglumine

1. NSAIDs inhibit the Cyclooxygenase (COX) enzyme that converts arachadonic acid into pro-inflammatory prostaglandin molecules, and helping prevent inflammation and subsequent pain. NSAIDs work primarily at the site of inflammation


2. What is the mechanism for analgesia from opioids like buprenorphine

2. Buprenorphine is a partial mu receptor agonist. Activation of mu receptors in the brain reduces the perception of painful stimuli. Opioids have little if any anti-inflammatory actions.


3. According to the Guide what is the definition of a major survival surgery?

3. A major survival surgery is defined as: Surgery that penetrates and exposes a body cavity or produces substantial impairment of physical or physiological function


Konkle et al. Environmental-Enrichment-Related Variations in Rats.T or F: increased plasma corticosterone may not necessarily reflect a negative condition but may allow the organism to better adapt to new situations



T or F: the chronic novelty and accrued sensory and cognitive experience associated with an enrichment paradigm may allow animals to be more resilient in the face of subsequent challenges



Serologic Prevalence of MPV1.MPV1f originated from
a. Murine cytotoxic T-cell line
b. Murine B-cell line
c. Murine tumour cell line
d. Naturally infected laboratory mice
e. None of the above

d. Naturally infected laboratory mice


True or False. 100 samples per room gives a 99% probability of detecting at least 1 seropositive mouse in the population if present at an expected prevalence of 5% only if all animals tested were not uniformly susceptible to the virus.



Of the following list, what is the mouse strain that poses difficulties of MPV antibody detection on ELISA tests?
a. DBA/2
b. BALB/c
c. C57BL/6
d. Hsd:NIH
e. Arc:Arc

b. BALB/c


Strain- and Age-Associated Variation to MPV-1.1. T/F: A negative antibody response to MPV for seroconversion in the BALB/cArc means that the mouse is negative for exposure to MPV1f.



2. T/F: If there is no viral DNA detected by PCR analysis then the animal was never exposed to the MPV1f.



3. Of the following which mouse is a better sentinel?
A. BALB/cArc
B. C57BL/6J
C. Arc:Arc(s)
D. BALB/cFox1nu/Arc

C. Arc:Arc(s)


Burkholder et al. Comparison of Carbon Dioxide and Argon Euthanasia.1. The CO2 displacement rate of 10% used in the study resulted in an increased time to euthanasia, but did not produce behavioral signs of distress in Sprague-Dawley rats, T/F?



2. Argon at 50% displacement rate for euthanasia resulted in behavioral abnormalities including seizures and agonal gasping, T/F?



Alves et al. Anesthesia with Intraperitoneal Propofol, Medetomidine, and Fentanyl in Rats.1. How does atipamezole reverse the effect of medetomidine?

1. Medetomidine is an alpha 2 agonist and atipamezole is an alpha 2 antagonist


2. Most current injectable anesthetic protocols for rats are associated with prolonged sedation with full recovery of consciousness taking perhaps several hours. T/F



3. Medetomidine combinations (e.g. combined with ketamine or fentanyl) have the advantage of faster recovery due to medetomidine reversal with atipamezole. However, ketamine combinations are associated with low-quality recoveries and the combination of medetomidine with only an opiate such as fentanyl tends to require a high dose of each to achieve anesthesia leading to adverse side effects (respiratory depression and polyuria) during the anesthetic episode. T/F



4. Although the use of inhalant anesthetics in rodents has become widespread, some procedures may not be amenable to the use of inhalant anesthetics (e.g. some orpoharyngeal procedures). T/F