JAALAS 2012 No. 4 Flashcards Preview

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Flashcards in JAALAS 2012 No. 4 Deck (38)
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1

Inbred strains are often used as animal models to avoid the confound of variations due to genetic background T/F

True

2

Although the exact differences in rearing environment between these 5 vendors was not elucidated in this study, what are some of the rearing variables one should consider?

Dietary, bedding, method of water delivery and treatment, caging system, microbiome, and the like.

3

T/F: Animals with a greater amount of enrichment in their home cages are more likely to develop stereotactic behaviors.

F (less likely)

4

Which of the following are proposed reasons for stereotypic behaviors? Pick all that apply.
a. CNS dysfunction
b. Frustration
c. Noise levels in the facility
d. Lack or removal of enrichment

A, B, and D

5

T/F: Stereotypic behaviors have traditionally been defined as those that are repetitive, invariant, and apparently serve some function.

F (serve no function)

6

1. What is the common name for Artemia?

Brine shrimp

7

2. Name one (1) research area for which zebrafish is commonly used.

Genetics; Developmental Biology

8

3. According to the article when are female zebrafish sexually mature?

3 months

9

T/F. The size of the female fish is a key factor in the amount of sperm released by male zebrafish during spawning.

True

10

1. What are the 7 principle functional units that were described in the article that the research group felt defined a state-of-the-art facility?

1. Core breeding unit, breeding-holding unit, phenotyping unit, archiving unit, quarantine unit, transgenic unit, and support infrastructure.

11

2. Which principle functional unit in most of the facilities has the highest hygiene level and access restrictions of the entire facility?

Core breeding unit

12

3. What were four concerns facilities had with their current or previous designs?

3. Size and capacity of holding or quarantine rooms, size and number of procedure rooms, support infrastructure (autoclave capacity, storage space), and lack of cost reductions.

13

1. How long did it typically take to detect EHS after dirty bedding exposure?
a. 2 weeks
b. 3 weeks
c. 4 weeks
d. 5 weeks

C

14

2. True or false: Monoinfections of EHS were more commonly found.

False

15

3. What was the most commonly identified EHS in the study?
a. H. bilis
b. H. rodentium
c. H. typhlonicus
d. H. hepaticus

D

16

1. What causes Chagas disease and how is it transmitted?

1. Trypanosoma cruzi; triatomine insect vectors (kissing bug) through contact of parasite containing feces with mammalian mucous membranes or through a break in skin

17

2. The first case of T. cruzi in NHP occurred where?

2. Covington, LA

18

3. What species of NHP could possibly be explored as an animal model for Chagas disease?

3. Pig-tailed macaques

19

4. T. cruzi can be transmitted from infected animals to caregivers and lab workers through contamination with infected blood? T or F

True

20

True or False: As defined by the USDA and the Animal Welfare Act, a painful procedure is any procedure that would reasonably be expected to cause more than slight or momentary pain or distress in a human being to which that procedure is applied.

True

21

True or False: Open-field measurements reflect responses to a novel environment and are less sensitive measures of wellbeing than are those obtained repeatedly or in the home cage.

False—they are a more sensitive measure.

22

Which of the following readily observable physical signs was a sensitive indicator of pain and distress after immunization with CFA or IFA in mice?
a. Body temperature
b. Erythema at immunization site
c. Swelling at immunization site
d. General physical activity
e. None of the above

E

23

Which of the following anesthetics targets both glycine and GABAA receptors?
a. Chloral hydrate
b. Pentobarbital
c. Urethane
d. All of the above

A

24

2. Which of the following anesthetics targets only the GABAA receptor?
a. Chloral hydrate
b. Pentobarbital
c. Urethane
d. All of the above

B

25

3. Which of the following anesthetics targets GABAA , glycine, and glutamate receptors?
a. Chloral hydrate
b. Pentobarbital
c. Urethane
d. All of the above

C

26

4. __________ anesthetized animals are thought to have similar physiologic and pharmacologic behaviors as unanesthetized animals.
a. Chloral hydrate
b. Pentobarbital
c. Urethane
d. Ketamine

C

27

True or False: Amphibians requires much higher concentrations of MS222 that due mammals.

True (reference 3)

28

MS222 stands for which of the following?
a. Eugenol
b. Benzocaine
c. Tricaine methanesulfonate
d. Tiletamine combined with zolazepam

C

29

What is the common name of Xenopus laevis?
a. African clawed toad
b. Bufo marinus
c. African clawed frog

C

30

Plethysmography is:
a. Used only to assess lung function.
b. Used only in small laboratory rodents.
c. Used to record changes in the size an organ.
d. Only effective when used on an anesthetized animal.

C