RNA transcription Flashcards
(13 cards)
Leader and tailing sequence
Immediately following the inverted methylated base 5’ cap.
Followed by start codon
Non translated
Immediately after the the stop codon
Followed by Poly A tail
Non translated
Hairpins
.
Start and Stop Codons
AUG - Start
UAG - Stop
UAA - Stop
UGA - Stop
RNA Polymerase
.
Holoenzyme
prokaryotic RNA poly enzyme consists of various subunits that comprise the holoenzyme
Core Enzyme
RNA poly subunits without Sigma subunit
Sigma Subunit
Recognizes the promoter regions
Amanitin (Alpha-Amantin)
mushroom poison
used to distinguish eukaryotic RNA polymerases
Rifampicin
antibiotic
used to distinguish eukaryotic RNA polymerases
Primary Transcript
pre-rRNA
Mature Transcript
pre-rRNA after modifications
- removal of both external and internal nucleotides by ribonucleases
- base modification
- addition of nucleotides.
Snurps
.
Ribonucleases
.