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MT6326 > 2S [LAB]: ASO > Flashcards

2S [LAB]: ASO Flashcards

1
Q

Principle of Anti-Streptolysin O titer test (tube test)

A

Neutralization

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2
Q

Principle of Anti-Streptolysin O slide test

A

Indirect/ passive agglutination

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3
Q

Positive result for ASO test

A

Absence of hemolysis

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4
Q

ASO titer that is considered normal

A

166 Todd units

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5
Q

ASO titer that is considered moderately elevated

A

at least 240 Todd units (adult)
at least 320 Todd units (child)

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6
Q

Concentration of the red cell suspension used for ASO test

A

5%

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7
Q

Positive result for ASO latex slide test

A

Agglutination

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8
Q

Principle of Streptozyme test

A

Passive hemagglutination

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9
Q

Positive result for Streptozyme test

A

Agglutination

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10
Q

Method commonly used in diagnosing recent post-streptococcal sequelae infections

A

Streptozyme test

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11
Q

Reagent used in Streptozyme Test

A

Aldehyde-fixed sheep red cells coated with exoenzymes

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12
Q

Morphology and gram stain reaction of Streptococcus

A

Gr (+) cocci in chains

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13
Q

Hemolytic toxins produced by S. pyogenes

A

Streptolysin O and S

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14
Q

Enzymes produced by S. pyogenes

A

Hyaluronidase, Deoxyribonuclease B (DNase), Streptodornase, Streptokinase

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15
Q

Virulence factor of S. pyogenes

A

M protein

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16
Q

Filamentous molecule from S. pyogenes that consists of two a-helical chains and limits the deposition of C3, therefore inhibiting complement activation

A

M protein

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17
Q

c is currently performed by ___

A

Nephelometric methods

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18
Q

Measures the ability of antibody in the patient’s serum to neutralize hemolytic activity of streptolysin O

A

Anti-streptolysin O titer test

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19
Q

A semi-quantitative procedure in which different dilutions of a patient’s serum are prepared and mixed with a fixed amount of streptolysin O antigen

A

Anti-streptolysin O titer test

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20
Q

Indicator particles used in Anti-streptolysin O titer test

A

Group O red cells

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21
Q

In Anti-streptolysin O titer test, what unit of measure is used if the streptolysin reagent standard is used?

A

Todd units

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22
Q

In Anti-streptolysin O titer test, what unit of measure is used if the WHO standard is used?

A

International units

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23
Q

In Anti-streptolysin O titer test, the streptolysin O reagent is reconstituted with ___ and should be used within ___

A

Distilled water
20 mins

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24
Q

In Anti-streptolysin O titer test, the preparation of phosphate-buffered saline (PBS) is done through dissolving the content of one small bottle of PBS granules in how much distilled water?

A

500 mL

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25
In Anti-streptolysin O titer test, what should be the result of tube 11?
No hemolysis (+)
26
In Anti-streptolysin O titer test, what should be the result of tube 12?
Presence of hemolysis (-)
27
In Anti-streptolysin O titer test, what is/are the component/s of the positive control?
1.5 mL PBS buffer
28
In Anti-streptolysin O titer test, what are the incubation conditions?
37C, 45 mins (before addition of RBCs) 37C, 15 mins (after addition of RBCs)
29
Centrifuge conditions in Anti-streptolysin O titer test
1 min, 1500 rpm
30
T/F: An Anti-streptolysin O titer test done on a single patient's sample is difficult to interpret or is of little diagnostic value unless the titer is extremely high
T
31
In Anti-streptolysin O titer test, ___ intervals for ___ weeks following streptococcal infection should be done to yield more meaningful information
bi-weekly 4-6 weeks
32
A single anti-streptolysin O titer of 125 does not provide much information, but if the titer rises from a baseline of ___, it is considered significant
50 units
33
In Anti-streptolysin O titer test, this suggests a current or a recent streptococcal infection that may progress into secondary or streptococcal sequelae infection
Titer rise from a baseline of 50 units
34
A drop in the ASO titer from 250 units to ___ is indicative of recovery
125 units
35
ASO titer typically increases within ___ weeks after infection & peaks between ___ weeks following initial symptoms
1-2 weeks 3-6 weeks
36
Normal range of antibody titer in Anti-streptolysin O titer test
0-125 units
37
A patient with rheumatic fever will demonstrate an average antibody titer of ___
500 IU/mL
38
Carrier molecule used in ASO latex slide test
Polystyrene latex spheres
39
T/F: In ASO latex slide test, it is important to allow all reagents and serum samples to reach room temperature before use
T
40
Dilution requirement of the patient's serum in ASO latex slide test
1:5
41
T/F: In ASO latex slide test, all samples, including the control sera are diluted
F (control sera should not be diluted)
42
In ASO latex slide test, the slide is tilted back and forth slowly for ___ after the addition of the reagent and sample
2 mins
43
A positive result in ASO latex slide test indicates that the ASO content in the patient's serum is more than ___
200 IU/mL + 15%
44
A rapid-multi-enzyme test that can simultaneously detect the antibodies of the different extracellular antigens of group A Streptococcus
Streptozyme test
45
T/F: The streptozyme test is a confirmatory test for GAS infection
F (screening)
46
Commonly used serological method in diagnosing recent post-streptococcal sequelae infections
Streptozyme test
47
In Streptozyme test, the mirrored slide is tilted back and forth for how many minutes and at what rate?
2 mins 8-10 times per min
47
Dilution requirement as starting dilution of the samples used in Streptozyme test
1:100
48
In Streptozyme test, the result is observed for agglutination within ___
10 s
49
T/F: Streptozyme test can be semiquantitative
T
50
A multienzyme test to detect Ab against different exoenzymes
Streptozyme test
51
Metabolic products of S. pyogenes that act as virulence factor
Exoenzymes
52
Most important exoenzyme of S. pyogenes
Streptolysin O
53
All of the following are protective mechanisms against bacteria except: i. Production of antimicrobial defense peptides ii. Phagocytosis iii. Activation of complement iv. Release of lipid A from the bacterial cell
iv
54
All of the following are characteristics of streptococcal M proteins except: i. It is the chief virulence factor of Group A streptococci ii. It provokes an immune response iii. Antibodies to one serotype protect against other serotypes iv. It limits phagocytosis of the organism
iii
55
An ASO titer and a streptozyme test are performed on a patient's serum. The ASO titer is negative, the streptozyme test is positive, and both the positive and negative controls react appropriately. What can you conclude from these test results? i. The ASO is falsely negative ii. The patient has an antibody to a streptococcal exoenzyme other than streptolysin O iii. The patient has not had a previous streptococcal infection iv. The patient has scarlet fever
ii
56
Which of the following applies to acute rheumatic fever? i. Symptoms begin after S. pyogenes infection of the throat of the skin ii. Antibodies to Group A streptocci are believed to cross-react with heart tissue iii. Diagnosis is usually made by culture of the organism iv. All patients suffer permanent disability
ii
57
Which of the following indicates the presence of anti-DNAse B activity in serum? i. Reduction of methyl green from green to colorless ii. Clot formation when acetic acid is added iii. Inhibition of RBC hemolysis iv. Lack of change in the color indicator
i
58
Which of the following is considered to be a nonsuppurative complication of streptococcal infection? i. Acute rheumatic fever ii. Scarlet fever iii. Impetigo iv. Pharyngitis
i
59
All of the following are ways that bacteria can evade host defenses except: i. Presence of a capsule ii. Stimulation of chemotaxis iii. Production of toxins iv. Lack of adhesion to phagocytic cells
ii
60
The following are dilutions required for ASO tube test except: i. 1:10 ii. 1:100 iii: 1:500 iv: 1:1000
iv
61
In the ASO tube test, which tube contains the 1:10 dilution?
Tube 1
62
In the ASO tube test, which tube contains the 1:100 dilution?
Tubes 2-6
63
In the ASO tube test, which tube contains the 1:500 dilution?
Tubes 7-10
64
In the ASO tube test, which tube contains the RBC control?
Tube 11
65
In the ASO tube test, which tube contains the SLO control?
Tube 12
66
T/F: In ASO tube test, the titer is derived from the last tube with hemolysis
F (no hemolysis)
67
In ASO latex test, what coats the latex particles?
SLO antigens
68
T/F: In ASO tube method, a single ASO tube test has no significant value
T
69
In ASO slide test, the dispense-stir can deliver how much reagent/serum?
40 uL
70
Detection limit of ASO slide test
>200 IU/mL +/- 15% (sabi ni arf) 166 Todd units
71
The following are detected by the streptozyme test except: i. Anti-SLO ii. Anti-O iii. Anti-streptokinase iii. Anti-DNAse B iv. Anti-hyaluronidase v. Anti-NADase
ii
72
Carrier particle used in streptozyme test
Sheep's RBC
73
Slide used in streptozyme test
Mirrored glass slide

MT6326 (28 decks)

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