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MT6326 > 2S [LAB]: Secondary Serologic Tests > Flashcards

2S [LAB]: Secondary Serologic Tests Flashcards

1
Q

Scientist who proposed the lattice hypothesis

A

Marrack

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2
Q

“Phase change reaction”

A

Precipitation

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3
Q

Serologic reaction that involves the interaction of a soluble Ag and an Ab

A

Precipitation

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4
Q

Antibody most commonly considered as precipitin

A

IgG

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5
Q

Method of precipitation detection:

Light scattering

A

Nephelometry

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6
Q

Method of precipitation detection:

Light transmitted

A

Turbidimetry

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7
Q

Medium used in precipitation reaction

A

1% Agarose gel (semi-solid medium)

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8
Q

Diffusion of Ag and/or Ab until it reaches the correct Ag-Ab proportion and then precipitates out of solution

A

Immunodiffusion

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9
Q

A single linear ID wherein known Ab is incorporated with gel is allowed to solidify. In this method, the sample is added by overlaying on top of solidified agar

A

Oudin test

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10
Q

A method of quantification of Ag by measurement of diameter of precipitation ring; samples are placed in wells

A

Single radial ID

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11
Q

In Single radial ID, agarose plate is coated with ___

A

Known Ab

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12
Q

In Single radial ID, the end point method is the ___

A

Mancini

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13
Q

In Single radial ID, the kinetic method is ___

A

Fahey McKelvey

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14
Q

A double ID method where wells are arranged at the apices of an imaginary equilateral triangle

A

Ouchterlony or Double Angular ID

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15
Q

A double ID method of determination of relationships of antigens in respect to Ab

A

Ouchterlony or Double Angular ID

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16
Q

A one-stage technique of single ID which measures the height of rocket

A

Laurell/ Rocket Immunoelectrophoresis

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17
Q

A technique where both Ag and Ab diffuse through an agarose gel until they meet at a point of equivalence

A

Double Simple ID

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18
Q

Immunofixation electrophoresis was first described by ___

A

Alper and Johnson

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19
Q

Immunofixation electrophoresis method where the antisera (Ab) is applied directly on the gel rather than placed in a through

A

Immunoprecipitation

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20
Q

Western, Southern, and Northern blotting are all examples of ___

A

Immunofixation electrophoresis

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21
Q

Serologic reactions involving particulate or insoluble Ag

A

Agglutination

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22
Q

Antibody most often considered as an agglutinin

A

IgM

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23
Q

Agglutination reaction where the Ag is inherently present on cell surface

A

Direct agglutination

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24
Q

Agglutination reaction applied on ABO typing

A

Direct agglutination

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25
Agglutination reaction applied on Widal Test
Direct agglutination
26
Agglutination reaction where the Ag is coated to carrier particle
Passive agglutination
27
Agglutination reaction where the Ab is coated to carrier particle
Reverse passive agglutination
28
T/F: The positive result for reverse passive agglutination is the absence of agglutination
False
29
Antiglobulin test that utilizes in vivo sensitization of RBCs
Direct Coombs/ Direct Antihuman Globulin Test
30
Antiglobulin test that utilizes in vitro sensitization of RBCs
Indirect Coombs/ Indirect Antihuman Globulin Test
31
Antibody utilized in the AHG reagent
IgG
32
Agglutination reaction that uses Staphylococcus aureus as carrier of Ab
Coagglutination
33
Positive result for complement fixation
No hemolysis
34
Reaction in which the antigenic activity is stopped/ nullified by a specific Ab
Neutralization
35
Principle of ASO tube test
Toxin Neutralization
36
Principle of Schick's Test
Toxin Neutralization
37
Principle of Dick's Test
Toxin Neutralization
38
Also known as natural clumping
Flocculation
39
A reaction that causes aggregation of colloidal particles and production of fleecy mass
Flocculation
40
Midway reaction between agglutination and precipitation
Flocculation
41
Principle of VDRL test
Flocculation
42
Non-cellular particulate Ag
Flocculation
43
T/F: IgG is an efficient Ab to fix complement
F
44
In coagglutination, the carrier particle used are bacterial cells pf which species?
Staphylococcus aureus
45
T/F: In RID for C3, the Ab is incorporated with the agarose gel
T
46
T/F: In reverse passive agglutination, Ab are attached to carrier particles
T
47
T/F: In neutralization, the positive result is hemolysis
F
48
T/F: Primary tests are less sensitive than secondary tests
F
49
Serologic reaction that involves the interaction of a soluble Ag and an Ab resulting in a precipitation reaction
Precipitation
50
A reaction that changes phase from soluble to insoluble
Precipitation
51
An example of liquid precipitation ring test method used for syphilis
Kahn test
52
An example of liquid precipitation ring test method used for anthrax
Ascoli test
53
Identify whether single or double ID and if 1- or 2-dimensional: Oudin test
Single ID, 1-D
54
Identify whether single or double ID and if 1- or 2-dimensional: Single Radial ID
Single ID, 2-D
55
Identify whether single or double ID and if 1- or 2-dimensional: Laurell Immunoelectrophoresis
Single ID, 1-D
56
Identify whether single or double ID and if 1- or 2-dimensional: Oakley Fulthrope
Double ID, 1-D
57
Identify whether single or double ID and if 1- or 2-dimensional: Ouchterlony or Double Angular ID
Double ID, 2-D
58
Identify whether single or double ID and if 1- or 2-dimensional: Double simple ID
Double ID, 2-D
59
Identify whether single or double ID and if 1- or 2-dimensional: Immunoelectrophoresis
Double ID, 2-D
60
Identify whether single or double ID and if 1- or 2-dimensional: Countercurrent immunoelectrophoresis
Double ID, 1-D
61
Identify whether single or double ID and if 1- or 2-dimensional: Immunofixation electrophoresis
Double ID, 2-D
62
In Laurell immunoelectrophoresis, what is placed in the well?
Sample with Ag
63
In the Oakley Fulthrope method, a positive result shows the precipitin band in which area?
Agar
64
In the Ouchterlony, how many wells are designated for Ag?
2
65
In the Ouchterlony, how many wells are designated for Ab?
1
66
Identify the technique based on the principle: Light that is scattered at an angle is measured, indicating the amount of antigen or antibody present
Nephelometry
67
Identify the technique based on the principle: Antigen diffuses out into gel that is infused with antibody. Measurement of the radius indicates concentration of antigen
Radial immunodiffusion
68
Identify the technique based on the principle: Both antigen and antibody diffuse out from well in a gel. Lines of precipitate formed indicate the relationship of antigens
Ouchterlony double diffusion
69
Identify the technique based on the principle: Electrophoresis of serum followed by diffusion of antibody from wells
Immunoelectrophoresis
70
Identify the technique based on the principle: Electrophoresis of serum followed by direct application of antibody to the gel
Immunofixation electrophoresis
71
T/F: Oudin test is a single ID
T
72
T/F: Agglutination involves the use of particulate Ag
T
73
T/F: IgM is an excellent agglutinin
T
74
T/F: ABO typing is an example of reverse hemagglutination
F
75
T/F: Ouchterlony involves application of electric current to allow movement of Ag and Ab
F

MT6326 (28 decks)

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