Applications of tissue processing Flashcards Preview

Year 1 Cell Biology > Applications of tissue processing > Flashcards

Flashcards in Applications of tissue processing Deck (66):
1

Chemical used for fixation

Formalin glutaraldehyde

2

Chemical used for dehydration

Ethanol (50% - 100%)

3

Chemical used for clearing

Xylene

4

Chemical used for embedding

Paraffin wax

5

Acid dyes have what net charge?

Negative

6

Basic dyes have what net charge?

Positive

7

Acidophilic tissues have what net charge?

Positive

8

Basophilic tissues have what net charge?

Negative

9

What is metachromasia?

A given stain imparts different colors to the tissue (e.g. mast cells stained with toluidine blue)

10

What is the structure affinity for Hematoxylin?

1) RNA2) DNA3) Ribosomes4) rER

11

What is the structure affinity for Eosin?

1) Secretory vesicles2) sER3) Lysosomes4) Mitochondria5) Type I collagen

12

What stain(s) could you use for RNA?

Hematoxylin

13

What stain(s) could you use for DNA?

Hematoxylin (blue), Feulgen reaction (magenta)

14

What stain(s) could you use for rER?

Hematoxylin

15

What stain(s) could you use for secretory vesicles?

Eosin

16

What stain(s) could you use for sER?

Eosin

17

What stain(s) could you use for lysosomes?

Eosin

18

What stain(s) could you use for mitochondria?

Eosin (pink), iron hematoxylin (dark blue to black)

19

What stain(s) could you use for Type I collagen?

Eosin

20

What is the structure affinity for Feulgen Reaction?

DNA

21

What is the structure affinity for Mallory Triple?

1) Nuclei (red)2) Muscle (red to orange)3) Collagen (blue)4) Hyaline cartilage (blue)

22

What stain(s) could you use for nuclei?

Mallory triple

23

What stain(s) could you use for muscle?

Mallory triple

24

What stain(s) could you use for collagen?

Mallory triple, Eosin (type I)

25

What stain(s) could you use for hyaline cartilage?

Mallory triple

26

What is the structure affinity for PAS Reaction?

Carbohydrates (magenta)

27

What stain(s) could you use for carbohydrates?

PAS reaction

28

What is the structure affinity for Osmic Acid?

Lipids (black)

29

What stain(s) could you use for lipids?

Osmic acid

30

What is the structure affinity for Verhoeff?

Elastic fibers (black)

31

What stain(s) could you use for elastic fibers?

Verhoeff

32

What is the structure affinity for Silver Methods?

Intermediate filaments of:1) Nerve cells (black)2) Glial cells (black)3) Reticular fibers (black)

33

What stain(s) could you use for intermediate filaments of nerve cells?

Silver Methods

34

What stain(s) could you use for intermediate filaments of glial cells?

Silver Methods

35

What stain(s) could you use for intermediate filaments of reticular fibers?

Silver Methods

36

What is the structure affinity for Trypan Blue?

Macrophages (blue)

37

What stain(s) could you use for macrophages?

Trypan Blue

38

What is the structure affinity for Prussian Blue?

Hemosiderin, ferric iron (blue)

39

What stain(s) could you use for hemosiderin?

Prussian Blue

40

What stain(s) could you use for ferric iron?

Prussian Blue

41

What is the structure affinity for Nissl?

Ribosomes (blue)

42

What stain(s) could you use for ribosomes?

Hematoxylin (blue), Nissl (blue)

43

What is the structure affinity for iron hematoxylin?

1) Nuclear elements (dark blue to black)2) Chromosomes (dark blue to black)3) Mitochondria (dark blue to black)4) Centrioles (dark blue to black)5) Muscle striation (dark blue to black)

44

What stain(s) could you use for nuclear elements?

Hematoxylin (blue), iron hematoxylin (dark blue to black), Feulgen Reaction (magenta), Mallory Triple (red)

45

What stain(s) could you use for chromosomes?

Iron hematoxylin

46

What stain(s) could you use for mitochondria?

Eosin (pink), iron hematoxylin (dark blue to black)

47

What stain(s) could you use for centrioles?

Iron hematoxylin (dark blue to black)

48

What stain(s) could you use for muscle striation?

Iron hematoxylin (dark blue to black)

49

What stain is used for coagulative necrosis?

H & E

50

What stain is used for thickened basement membranes in kidney disease?

PAS Reaction

51

What stain is used for glycogen storage disorders?

PAS Reaction

52

What stain is used for alpha 1 - antitrypsin deficiency in liver cells?

PAS Reaction

53

What stain is used for fibrosis?

Mallory Triple

54

What stain is used to detect nuclear changes in cancer?

Feulgen Reaction

55

What stain is used to detect excessive iron accumulation in hematochromatosis?

Prussin Blue

56

What stain is used to detect extracellular deposits of amyloid?

Congo Red

57

What stain is used to detect elastic fibers in Marfan Syndrome?

Verhoeff

58

Which is more sensitive - direct or indirect IHC?

Indirect (more antibodies)

59

What are four clinical utilities of IHC?

1) Categorization of tumor origin and aggressiveness 2) Estrogen receptors in breast cancer3) HER2 / Neu in breast cancers and adenocarcinomas4) Carcinoembryonic antigen in breast and intestinal cancers

60

What does FISH detect / identify?

A nucleic acid sequence of interest

61

What does FISH use to identify a nucleic acid sequence of interest?

Complementary nucleic acid probes

62

What method would be used to detect a cell infected with a virus (e.g. HPV2)?

FISH

63

What method would be used to detect specific genes on a chromosome (e.g. BRCA1 / BRCA2)?

FISH

64

What method would be used to detect amplification of genes (e.g. myc, HER2 / Neu)?

FISH

65

What are three clinical utilities of FISH?

1) Detect cells infected with a virus2) Detect specific genes on a chromosome3) Detect amplification of genes

66

What disease is characterized by teenage blindness and hairy palms?

Kevin Ehlers Disease