Bio Semester 2 Final Flashcards
This deck was created by combining two or more decks (45 cards)
Splicing
The removal of introns and the making of all the Exons together.
Exons
Good RNA that is out together and codes for proteins.
Translation
Nucleus acid code to AA code
Codon
mRNA code
Anticodon
tRNA code
Gel electrophoresis
Uses charge and size to separate and sort DNA segments. The higher the charge the smaller the size. Smaller DNA fragments with move farther. And then we can separate them by size.
Restriction enzymes
Cuts out part of DNA from plasmid
Use ligase to glue that DNA into another plasmid
Starts making proteins with new DNA
Uracil
Is used instead of thymine for tRNA only.
Deoxyribose
Make up DNA
Purine
Two fused rings
Adenine and guanine
Polymerase chain reaction
Put DNA polymerase with nucleotides and heat. They make more DNA which is the point of the experiment.
Pyrimidines
Single fused ring
Cytosine
Thymine
Uracil
Mutations
Only changing one gene within a chromosome
Ribose
Makes up RNA
Gene cloning
Take plasmid out of bacteria
Our in another so it can absorb DNA.
Put plasmid back in other bacteria
What is in a nucleotide?
Base pair
Phosphate group
Deoxyribose
Introns
Bad RNA that is removed
What are the 3 parts of interphase and what do they do?
G1 - cell grows and does its thing
S or synthesis - DNA duplicates
G2 - DNA is loose chromatin but ready to go
Somatic cells
Body cells and they are diploid
Prophase
Chromatin condenses and can be seen by microscope. Nuclear envelope breaks down. Spindle fibers start to form and attach themselves to kinetochore.
Differences between meiosis and mitosis
Mitosis ends up with 2 complete set of chromosomes, one per cell. However, in meiosis, those homologous chromosomes split again to separate daughter cells.
Spindle fibers
Made of microtubules. They connect at the kinetochore to the to poles of the cell
Centrioles
Specialized structures that we don’t know what they do exactly. Something with the production of spindle fibers.
What is mitosis?
The duplicating and movement of chromosomes to the opposite sides of a cell, ultimately forming 2 daughter nuclei.