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Flashcards in Facility Design and Management Deck (78)
1

define biosecurity

measures taken to detect, prevent, contain, and eradicate adventitious infections

2

define programming in terms of facility design

decision-making phase that defines the scope of the building project

3

what is in the program document in terms of facility design

describing in detail what research will be housed in the facility and its support requirements. ie space allocation summary, list of all square footage requirements

4

what are the pros and cons of a multi corridor system vs a single corridor system

multi cooridor system allows for better separation of clean and soiled materials but requires more space.

5

what are 4 types of animal housing areas?

conventional, quarantine, biocontainment, barrier

6

typical modular rodent room sizes

11ft x 22ft

7

what equipment should be fixed in an animal holding room?

sink, no casework, as open and flexible as possible

8

define barrier

refers to facilities and management practices designes to isolate animals from infectious agents

9

levels of barrier

typically at the cage level. if using open top caging, may be at room level, isolators

10

standard rodent cage capacity of rooms

500-800 cages equates to 250-400 sq ft

11

most common cubicle size

4ft deep x 6 ft wide

12

benefits of cubicles

allows greater separation of animals by pecies, source, microbiological status, project, and experimental hazards.

13

air distribution in cubicle set up

asile is positive to cubicle. air from cubicle enters when door open and can then be forced into other cubicles when pressure is restored.

14

NHPs are housed under what biohazard status

ABSL2

15

special componenets of NHP housing

entry vestibule, lights and fixtures designed and secured so that animals free in the room cannot damage them, trough drain system, hose bibs

16

slope of floor for ideal drainage

3/16in per foot from crown to floor trough. slope in trough 1/4in per foot to drain

17

size of drain

4in minimum, should be 6in if will be washing down waste

18

ratio of support space to animal housing space

30:70 to 70:30

19

engineering features required when dealing with hazardous materials

physical isolation of animals and waste, room surfaces are monolithic, sealed, and easily sanitized, use of BSCs or chemical hood, increased room air exchange rates to dilute contaminants, directional airflow, room pressure differentials

20

chemical hoods provide protection for what? personnel, product, neither, both?

personnel only

21

BSC Class II Type B2 provide protection for what? personnel, product, neither, both?

both

22

how long should caracaases and waste be allowed to decay before disposal into nonradioactive waste streams

10 half lives

23

define cleaning

removal of organic and nonorganic debris adhering to surfaces

24

define disinfection

reduces but does not eliminate microorganisms

25

define Sanitation

combination of cleaning and disinfection

26

define sterilization

eliminates all life forms

27

preferred method of sanitiation of cages and equipment

mechanical washers and water at temps in excess of 180F

28

describe the set up of a constant volume reheat type of HVAC system

CVR system draws in 100% fresh air, then filter, heat or cool, and humidify before distributing it within the facility. Conditioned air delivered to individual rooms at a constant temperature and humidity is then reheated to the desired temperature at the room level based on settings of rooms thermostat

29

describe the set up of a variable air volume system?

ventilation rates can be tailored to maintain desired environmental conditions

30

final particulate filters found on most vivarium air handles filter what?

to 85-95% dust spot efficiency

31

Filtering ability of HEPA filter

99.97% of 0.3um diameter particles

32

what systems are essential to have on emergency power?

ventilated caging, aquatic life support, environmental monitoring and security systems

33

common air pressure differentials used

0.02-0.08 inches of water column

34

air velocities in a room should not exceed what level, especially when not using filter top caging?

0.25m/s

35

set point for rodent handling rooms?

72-72F

36

which of the environmental parameters is the most challenging to maintain? the most important to maintain?

humidity
temperature

37

acceptable daily temperature change

plus or minus 2F

38

what is the intracage light intensity differential between cages at the top of the rack and those at the bottom?

80 fold difference

39

recommendation for light intensity at cage level

between 130-325 lux.

40

recommended max noise in animal facilities

85 dB

41

at a minimum, which parameters should be continuously monitored

temperature and light periodicity

42

in the event of a thermostat or heating system malfunction, the reheat coil should....

fail closed to prevent room overheating

43

what is the relationship between MaE RH and ammonia

Higher MaE RH = higher MiE RH = earlier ammonia is detectable after cage change.

44

why is ammonia not a concern when housing axenic or gnotobiotic animals?

flora is devoid of urease producing bacteria

45

what is the standard acceptable level for MiE ammonia concentration

there is none but no more than 25ppm is the performance standard.

46

benefits of static MI cages

good for containment of disease and hazards

47

cons of static MI cages

microenvironment begins to deterioriate rapidly, requiring more frequent cage changes

48

benefits of MI

better microenvironment less frequent cage changes, variability in MiE air quality is reduced or eliminated when compared to static cages, able to increase housing capacity- take up less space than static caging

49

types of systems of IVCs for air exchange

perimeter capture, direct, indirect, combination

50

ideal intracage ventilation rate

60ACH with cage change q 14 days

51

air speed at locations that cage occupants would encounter

less than 50 linear feet per minute

52

cons of IVCs

if high velocity, can lead to chilling, pheromone dilution esp with breeding mice

53

additional condsiderations with IVC equipment

heat load, exhaust release, noise generation, power requirements and failure, vibration, blower maintenance and calibration, and sanitation

54

sanitation frequency for IVC

no consensus

55

most common type of mass air displacement device found in a lab animal facility

cage change station or isolator

56

what type of air is supplied when using a MAD

Class 100 air, air that contains no more than 100 (0.5um particles or larger per cubic foot of air.

57

Class 1 stations, use and protection provided?

bedding dump stations, personnel protection only

58

Class II Type A, exhaust is handled how?

air is recirculated

59

Class II Type B, exhaust is handled how?

hard ducted to the buildings exhaust.

60

feed comes in to forms

pelleted and extruded

61

natural diets come in two forms

open and closed

62

define a closed diet

individual components of the diet are not provided but a guaranteed analysis is

63

what is an open formula diet

manufactured in accordance with an established known ingredient formulation

64

define purified diet

formulated from refined ingredients

65

define chemically defined diets

formulated from chemically pure compounds

66

when might you use a purified or chemically defined diet and what is one of the main drawbacks?

when you are altering the nutritional content or need to alter a specific dietary component. typically less palatable than natural ingredient diets.

67

ways to sterilize food

autoclave and gamma irridiation

68

define certified feeds

analyzed and certified to contain not more than the established maximum level of environmental contaminants, including heavy metals, chlorinated hydrocarbons, organoposphates, aflatoxins.

69

storage conditions for natural ingredient diets

70C 50% humidity

70

ways to disinfect large quantities of water, ie aquatic systems

UV or ozone

71

additives for rodent drinking water

hydrochloric acid, chlorine

72

ideal pH for using acidification for purification

2.5-3

73

ideal ppm of chlorine for hyperchlorination for purification

0.5-10ppm (6-8 ideal but should be less than 10)

74

the bottom shelf of rodent racks should be no less than ____ off the floor

6in

75

what are the four plastics used for rodent caging

polycarbonate, polypropylene, polystyrene, polyethylene

76

what plastic for rodent caging is the most durable

polycarbonate

77

what plastic for rodent caging is the most cost effective

polystyrene

78

what plastic for rodent caging is disposable

polyethylene