Flashcards in mouse immunodeficiency models Deck (24)
1
what mutation is present in nude mice?
Foxn1 nu
2
what mutation is present in SCID mice?
Prkdc scid
3
deficiencies seen in nude mice
decreased T cells, but leaky
4
deficiencies seen in SCID mice?
decreased T and B cells, but leaky
5
deficiencies seen in SCID beige mice
decreased T, B and NK cell activity
6
deficiencies seen in NOD-SCID mice
decreased T, B, and NK activity, minimal leaky
7
deficiencies seen in NSG, or NOG mice
decreased T, B and NK activity, not leaky
8
what are the common medical complications seen in nude mice?
females fail to lactate
susceptible to Corynebacterium bovis
susceptible to Staph xylosus
ocular issues
lack of whiskers
9
what chromosome is the SCID mutation on?
Chr 16
10
what immunodeficiency is seen in the SCID mouse?
defective DNA dependent kinase coding for TCR and B cell receptors
11
what chromosome is the Rag mutation on?
Chr 2
12
what chromosome is the Beige mouse mutation on?
Ch 13
13
what is the cause of the immunodeficiency seen in the Beige mouse?
lysosomal storage disease
14
what is the cause of the immunodeficiency seen in the XID mouse?
defective Bruton's tyrosine kinase (signal transduction in B cells)
15
what immunodeficiency seen in the moth eaten mouse?
lacks cytotoxic T and NK cells
16
what is the cause of the immunodeficiency seen in the moth eaten mouse?
defective phosphatase, Chr 6
17
list two mouse models for systemic lupus erythematosus
NZB x NZW F1 hybrid
FAS lpr mouse
18
what chromosme is the Foxn1 (nude mouse) mutation on?
Chr 11
19
pathophysiology of immunodeficiency in nude mide
dysgenesis of thymic epithelium, lack measurable T cell activity, increased NK cell activity
20
major uses of nude mice
oncology, xenografts
21
What immunodeficiency is seen in the Rag1 or 2 knockouts?
Antibody and T cell deficient, not leaky
22
What chromosome is the Rag 1 and 2 found on?
Chr 2
23
breeding of Foxn1nu animals
breed nude males with heterozygous females
24