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Flashcards in mouse immunodeficiency models Deck (24)
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1
Q

what mutation is present in nude mice?

A

Foxn1 nu

2
Q

what mutation is present in SCID mice?

A

Prkdc scid

3
Q

deficiencies seen in nude mice

A

decreased T cells, but leaky

4
Q

deficiencies seen in SCID mice?

A

decreased T and B cells, but leaky

5
Q

deficiencies seen in SCID beige mice

A

decreased T, B and NK cell activity

6
Q

deficiencies seen in NOD-SCID mice

A

decreased T, B, and NK activity, minimal leaky

7
Q

deficiencies seen in NSG, or NOG mice

A

decreased T, B and NK activity, not leaky

8
Q

what are the common medical complications seen in nude mice?

A
females fail to lactate
susceptible to Corynebacterium bovis
susceptible to Staph xylosus
ocular issues
lack of whiskers
9
Q

what chromosome is the SCID mutation on?

A

Chr 16

10
Q

what immunodeficiency is seen in the SCID mouse?

A

defective DNA dependent kinase coding for TCR and B cell receptors

11
Q

what chromosome is the Rag mutation on?

A

Chr 2

12
Q

what chromosome is the Beige mouse mutation on?

A

Ch 13

13
Q

what is the cause of the immunodeficiency seen in the Beige mouse?

A

lysosomal storage disease

14
Q

what is the cause of the immunodeficiency seen in the XID mouse?

A

defective Bruton’s tyrosine kinase (signal transduction in B cells)

15
Q

what immunodeficiency seen in the moth eaten mouse?

A

lacks cytotoxic T and NK cells

16
Q

what is the cause of the immunodeficiency seen in the moth eaten mouse?

A

defective phosphatase, Chr 6

17
Q

list two mouse models for systemic lupus erythematosus

A

NZB x NZW F1 hybrid

FAS lpr mouse

18
Q

what chromosme is the Foxn1 (nude mouse) mutation on?

A

Chr 11

19
Q

pathophysiology of immunodeficiency in nude mide

A

dysgenesis of thymic epithelium, lack measurable T cell activity, increased NK cell activity

20
Q

major uses of nude mice

A

oncology, xenografts

21
Q

What immunodeficiency is seen in the Rag1 or 2 knockouts?

A

Antibody and T cell deficient, not leaky

22
Q

What chromosome is the Rag 1 and 2 found on?

A

Chr 2

23
Q

breeding of Foxn1nu animals

A

breed nude males with heterozygous females

24
Q

aside from the NZB/NZW F1, what is another model for SLE

A

Fas(lpr) on chromosome 19, leads to failure of lymphocytes to undergo apoptosis