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Flashcards in mouse immunodeficiency models Deck (24)
1

what mutation is present in nude mice?

Foxn1 nu

2

what mutation is present in SCID mice?

Prkdc scid

3

deficiencies seen in nude mice

decreased T cells, but leaky

4

deficiencies seen in SCID mice?

decreased T and B cells, but leaky

5

deficiencies seen in SCID beige mice

decreased T, B and NK cell activity

6

deficiencies seen in NOD-SCID mice

decreased T, B, and NK activity, minimal leaky

7

deficiencies seen in NSG, or NOG mice

decreased T, B and NK activity, not leaky

8

what are the common medical complications seen in nude mice?

females fail to lactate
susceptible to Corynebacterium bovis
susceptible to Staph xylosus
ocular issues
lack of whiskers

9

what chromosome is the SCID mutation on?

Chr 16

10

what immunodeficiency is seen in the SCID mouse?

defective DNA dependent kinase coding for TCR and B cell receptors

11

what chromosome is the Rag mutation on?

Chr 2

12

what chromosome is the Beige mouse mutation on?

Ch 13

13

what is the cause of the immunodeficiency seen in the Beige mouse?

lysosomal storage disease

14

what is the cause of the immunodeficiency seen in the XID mouse?

defective Bruton's tyrosine kinase (signal transduction in B cells)

15

what immunodeficiency seen in the moth eaten mouse?

lacks cytotoxic T and NK cells

16

what is the cause of the immunodeficiency seen in the moth eaten mouse?

defective phosphatase, Chr 6

17

list two mouse models for systemic lupus erythematosus

NZB x NZW F1 hybrid
FAS lpr mouse

18

what chromosme is the Foxn1 (nude mouse) mutation on?

Chr 11

19

pathophysiology of immunodeficiency in nude mide

dysgenesis of thymic epithelium, lack measurable T cell activity, increased NK cell activity

20

major uses of nude mice

oncology, xenografts

21

What immunodeficiency is seen in the Rag1 or 2 knockouts?

Antibody and T cell deficient, not leaky

22

What chromosome is the Rag 1 and 2 found on?

Chr 2

23

breeding of Foxn1nu animals

breed nude males with heterozygous females

24

aside from the NZB/NZW F1, what is another model for SLE

Fas(lpr) on chromosome 19, leads to failure of lymphocytes to undergo apoptosis