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Microbiology > HBB > Flashcards

HBB Flashcards

1
Q

Haemophilus Features

A

Non motile
Non sporing
Gram -ve
Oxidase +ve

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2
Q

Oxidase Positive Organisms

A

PVNCH
Pseudomonas
Vibrio
Neisseria
Campylobacter
HBB (Hemophilus, Bordetella and Brucella)

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3
Q

H. Influenzae also known as

A

Pfeiffer’s bacillus

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4
Q

H. Influenzae requires which factors

A

X and V

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5
Q

Virulence factors of H influenzae

A

Capsular polysaccharide - 6 types (A to F)
H1b - Most virulent (used for H1b PRP vaccine)

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6
Q

H. Influenzae diseases ranges in humans from

A

Invasive and
Non invasive

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7
Q

Capsule in case of Invasive and Non invasive H. Influenzae

A

Invasive - encapsulated
Non Invasive - Non encapsulated

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8
Q

Disease spread in Invasive and Non invasive H influenzae

A

Invasive - Blood
Non invasive - locally

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9
Q

H1b seen in which form of H influenzae

A

Invasive

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10
Q

Blood culture will be positive in which form of H influenzae

A

Invasive

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11
Q

Infections caused by invasive form of H influenzae

A

Meningitis
Sepsis
Pneumonia

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12
Q

Infections caused by Non invasive form of H influenzae

A

Otitis media
Sinusitis
Exacerbation of COPD

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13
Q

Clinical features related to H influenzae

A

Meningitis
Croup (Laryngotracheobronchitis)
Pneumonia
Suppurative lesions

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14
Q

Microscopic Findings in Case of H influenzae

A

Gram negative Coccobacilli

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15
Q

Culture findings in case of H influenzae

A

X factor (hemin)
V factor (NAD Or NADP)

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16
Q

Satellitism shown on blood agar by which organism

A

H. Influenzae

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17
Q

Treatment of H influenzae

A

Cefotaxime or Ceftriaxone

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18
Q

Precaution or vaccine for H influenzae

A

H1b PRP Vaccine

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19
Q

H. Aegypticus is also known as

A

Koch weaks bacillus

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20
Q

H Aegypticus causes

A

Pink eyes
Brazilian purpeuric fever (Conjunctivitis leading to septicemia)

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21
Q

H Ducreyi causes

A

Chancroid - Painful soft Genital ulcers

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22
Q

Culture media for H Ducreyi

A

Grows on chocolate agar supplemented with Isovitalex or fetal calf serum

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23
Q

Staining and tissue biopsy finding for H Ducreyi

A

Bipolar staining (Safety pin appearance)
Tram track/School of fish appearance

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24
Q

Treatment of H. Ducreyi infection

A

Azithromycin

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25
Q

Organisms containing Only Factor X

A

Dus - H. Ducreyi

26
Q

Organism containing only Factor V

A

Paanch - Para
H. Parainfluenza
H. Parahemolyticus
H. Paraphrophilus

27
Q

Organisms containing both Factor X and V

A

BHAI
B - Both
H - H. Hemolyticus
A - H. Aegypticus
I - H. Influenzae

28
Q

Normal mouth Commensals or HACEK organisms

A

H - Haemophilus
A - Aggregatibacter
C - Cardiobacterium
E - Eikenella
K - Kingella

29
Q

HACEK are known to cause

A

Endocarditis
Respiratory infections
UTI

30
Q

Bordetella features

A

Non motile
Non sporing
Capsule +

31
Q

Bordetella pertussis causes

A

Pertussis/Whooping cough/100 day cough

32
Q

Virulence factors of Bordetella pertussis

A

Agglutinogens - helps in attachment
Filamentous hemagglutinin (FHA)
Pertactin - outer membrane protein

33
Q

Filamentous hemagglutinin (FHA) Is used for

A

Used in Acellular pertussis vaccine

34
Q

Toxin responsible for Bordetella pertussis infection

A

Pertussis toxin

35
Q

Pertussis toxin exhibits

A

LPF (Lymphocytosis producing factor)
HCF (Histamine secretizing Factor)
IAP (Islet activating protein) - Hypoglycemia

36
Q

Pertussis MOA

A

Increases adenylate cyclase (cAMP)

37
Q

Incubation period of Bordetella pertussis

A

1-2 weeks

38
Q

Phases of Bordetella pertussis infection

A

Catarrhal - low grade fever, Dry cough, Max infectivity
Paroxysmal - Bouts of cough, inrush of air into lungs (whoop)
Convalescent - lesser severity

39
Q

Swabs are transported in which media in case of Bordetella pertussis

A

Casamino acid solution/Stuart media

40
Q

Specimen taken for Bordetella infection

A

Nasal swab, Per nasal, Per oral
6 swabs takes
Dacron or Calcium alginate swabs are used ( not cotton)

41
Q

Culture media used for diagnosis of Bordetella pertussis

A

Bordet gengou medium - Shows Mercury drop/Bissected pearls appearance

42
Q

Bordetella pertussis Mnemonic

A

Bord - Body (capsule)
Bordet Gengou medium
B and P - Bissected pearls

43
Q

Treatment of Bordetella pertussis infection

A

Erythromycin, Azithromycin

44
Q

Prevention of Bordetella pertussis infection

A

1) Whole cellular vaccine (FHA, Pertactin, Agglutinogens, Pertussis toxin) - No complications
2) Whole cell killed vaccine

45
Q

Complication of whole cell killed vaccine give for Pertussis

A

Post vaccinal encephalopathy

46
Q

Classification of Brucella

A

NOMEN System
MASC-ON
M - Melitensis
A - Abortus
S - Shis
C - Canis
O - Ovis
N - Neutamae

47
Q

Brucella Melitensis

A

Sheep/goat
Most pathogenic
M antigen

48
Q

Antigen seen in Brucella Abortus

A

A Antigen

49
Q

Virulence factors of Brucella

A

LPS
BvrR/BurS - binding

50
Q

Transmission of Brucella infection

A

Zoonotic disease
M/c direct contact > raw milk

51
Q

Clinical features of Brucella infection

A

Goes to reticuloendothelial cells
Night sweats
Hepatosplenomegaly
Arthritis

52
Q

Incubation period of Brucella

A

10-30 days

53
Q

Types of infection caused by Brucella

A

1)Latent - no clinical evidence, Serology +ve
2)Acute/Subacute - Fever, Arthralgia, Nocturnal sweating, Low platelet count (Bleeding)
3)Chronic - low grade

54
Q

Medium used for diagnosis of Brucella

A

Castaneda medium (biphasic) -;Solid and liquid

55
Q

Brucella is Catalase, Oxidase and Urease

A

Positive

56
Q

Which agglutination test is shown by Brucella

A

Standard agglutination test

57
Q

Test done on animals in case of Brucella

A

Milk ring test - blue or purple ring at top
Bengal card test
Whey agglutination test

58
Q

Treatment of Brucella infection

A

Doxycycline × 45 days + Streptomycin im daily for 14 days

59
Q

Treatment recommended by WHO for Brucella

A

Doxycycline + Rifampicin for 6 weeks

60
Q

Treatment for Neurobrucellosis

A

Doxycycline + Rifampicin + Ceftriaxone

Microbiology (37 decks)

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