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Microbiology > Mycology - Introduction And classification > Flashcards

Mycology - Introduction And classification Flashcards

1
Q

Fungus cell membrane and cell wall composition

A

Cell wall - Chitin
Cell membrane - Ergosterol except Pneumocystis carinii/jiroveci

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2
Q

Classification of Fungus based on Morphology

A

1) Yeast - Budding form
2)Yeast like - budding + pseudohyphae
3) Mold/Hyphae
4) Dimorphic fungi

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3
Q

Yeast form of fungus and examples

A

Shows Budding
Ex - Cryptococcus, S. Cerevicae

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4
Q

Yeast like form Examples

A

Pseudohyphae + Budding
Ex - Candida, Malassezia

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5
Q

Mold/Hyphae form is Further classified into

A

Aseptate
Septate

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6
Q

Example of aseptate Mold/hyphae form

A

Zygomycetes

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7
Q

Pigments of Septate mold/hyphae form and examples

A

Phacoid/Melanin - Piedra, Alternaria,Madurella, Curvellaria
Hyaline - Fusarium, acremonium, Aspergillus, Penicillium

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8
Q

Dimorphic fungi exists in which morphologies

A

Yeast @ 37°C and
Mold/Hyphae @ 25°C

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9
Q

Examples of Dimorphic fungus

A

Body Heat Probably changes shape
Body - Blastomyces
Heat - Histoplasmosis
Probably - Penicillium marnefii, Paracocci Iodomycosis
Change - Coccidioidomycosis
Shape - Sporotrichosis

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10
Q

Classification of fungi based on spores

A

Sexual spores
Asexual spores

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11
Q

Sexual spores Includes

A

ZABD
Zygospores
Ascospores
Basidiospores
Deuteromycetes

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12
Q

Zygospores is seen in

A

Zygomycetes

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13
Q

Ascospores are seen in

A

Aspergillus

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14
Q

Basidiospores are seen in

A

Cryptococcus

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15
Q

Deuteromycetes shows

A

No sexual spores - Fungi imperfecta

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16
Q

Asexual spores examples

A

Arthrospores
Blastospores
Sporangiospores
Chlamydospores
Conidia

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17
Q

Arthrospores are seen in

A

Condensation of hyphael elements
Coccidioidomycosis
Dermatophytes

18
Q

Sporangiospores Are seen in

A

Zygomycetes

19
Q

Chlamydospores are seen in

A

Shows Double wall
Candida

20
Q

Conidia are classified into

A

Macroconidia
Microconidia

21
Q

Zygomycetes shows which spores

A

Zygospores
Sporangiospores

22
Q

Vegetative states shown by fungus

A

Spiral hyphae
Rectate body (like comb)
Favic Chandelier/Antler hyphae
Nodular organ
Racquest hyphae

23
Q

Spiral hyphae shown in

A

Trichophyton Mentagrophytes

24
Q

Favic Chandelier/Antler Hyphae examples

A

Trichophyton Schoenleinii

25
Racquest hyphae seen in
Epidermophyton floccosum
26
Classification of Fungi on basis of location
Superficial Mycosis Subcutaneous Mycosis Deep/Systemic Mycosis
27
Superficial Mycosis Causative organisms
Tinea versicolor Dermatophytes
28
Subcutaneous Mycosis causes
MRCS Mycetoma Rhinosporidiosis Chromoblastomycosis Sporotrichosis
29
Deep Mycosis/systemic Mycosis causes
Dimorphic fungi Opportunistic infections - Candida, Cryptococcus, Aspergillus, Mucor
30
Sample taken for diagnosis of Fungal infections
Hair, skin, Nail Pus Lung - sputum, Bronchial washings, Lung biopsy Blood CSF urine Body fluids
31
Chemical used for Direct examination on M/E
10-20% KOH Mount - digest keratin
32
Stains used for diagnosis of Fungal infections
GMS (Gomori Methanamine Silver stain) - Black Per-iodic acid Schiff - Pink Gram stain H and E stain Calcoflour white - Fluorescent stain Negative stain Mucicarmine
33
Fluorescent stain used for Fungus
Calcoflour white
34
Negative stain used for diagnosis of which organism
India ink, Nigrosin Used for Cryptococcal Meningitis - CSF sample
35
Stain used for Diagnosis of Cryptococcal Meningitis in case of Biopsy sampe
Mucicarmine
36
Culture used for diagnosis of Fungal infections
Sabouraud Dextrose agar (SDA) Corn meal Agar Bird seed Agar/Niger agar Chrom agar
37
Sabouraud Dextrose agar Composition and pH
2% Dextrose pH - 5.6 (Acidic) Antibiotics - Gentamicin, Chloramphenicol (kills bacteria) Cycloheximide - to kill saprophytic fungi
38
Stain used for Sabouraud Dextrose agar
Lactophenol cotton blue (LPCB)
39
Lactophenol cotton blue composition
Lactic acid - Preserves Morphology Phenol - Disinfectant Glycerol - Prevent drying Cotton blue - Dye Used to study culture morphology
40
Corn meal agar used to grow
Chlamydospores - Candida
41
Cultures used for Candida
Corn meal agar Chrom agar
42
Bird seed agar/Niger agar used for which organism
Brownish color Cryptococcus

Microbiology (37 decks)

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