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Microbiology > Mycology - Introduction And classification > Flashcards

Mycology - Introduction And classification Flashcards

1
Q

Fungus cell membrane and cell wall composition

A

Cell wall - Chitin
Cell membrane - Ergosterol except Pneumocystis carinii/jiroveci

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2
Q

Classification of Fungus based on Morphology

A

1) Yeast - Budding form
2)Yeast like - budding + pseudohyphae
3) Mold/Hyphae
4) Dimorphic fungi

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3
Q

Yeast form of fungus and examples

A

Shows Budding
Ex - Cryptococcus, S. Cerevicae

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4
Q

Yeast like form Examples

A

Pseudohyphae + Budding
Ex - Candida, Malassezia

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5
Q

Mold/Hyphae form is Further classified into

A

Aseptate
Septate

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6
Q

Example of aseptate Mold/hyphae form

A

Zygomycetes

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7
Q

Pigments of Septate mold/hyphae form and examples

A

Phacoid/Melanin - Piedra, Alternaria,Madurella, Curvellaria
Hyaline - Fusarium, acremonium, Aspergillus, Penicillium

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8
Q

Dimorphic fungi exists in which morphologies

A

Yeast @ 37°C and
Mold/Hyphae @ 25°C

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9
Q

Examples of Dimorphic fungus

A

Body Heat Probably changes shape
Body - Blastomyces
Heat - Histoplasmosis
Probably - Penicillium marnefii, Paracocci Iodomycosis
Change - Coccidioidomycosis
Shape - Sporotrichosis

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10
Q

Classification of fungi based on spores

A

Sexual spores
Asexual spores

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11
Q

Sexual spores Includes

A

ZABD
Zygospores
Ascospores
Basidiospores
Deuteromycetes

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12
Q

Zygospores is seen in

A

Zygomycetes

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13
Q

Ascospores are seen in

A

Aspergillus

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14
Q

Basidiospores are seen in

A

Cryptococcus

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15
Q

Deuteromycetes shows

A

No sexual spores - Fungi imperfecta

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16
Q

Asexual spores examples

A

Arthrospores
Blastospores
Sporangiospores
Chlamydospores
Conidia

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17
Q

Arthrospores are seen in

A

Condensation of hyphael elements
Coccidioidomycosis
Dermatophytes

18
Q

Sporangiospores Are seen in

A

Zygomycetes

19
Q

Chlamydospores are seen in

A

Shows Double wall
Candida

20
Q

Conidia are classified into

A

Macroconidia
Microconidia

21
Q

Zygomycetes shows which spores

A

Zygospores
Sporangiospores

22
Q

Vegetative states shown by fungus

A

Spiral hyphae
Rectate body (like comb)
Favic Chandelier/Antler hyphae
Nodular organ
Racquest hyphae

23
Q

Spiral hyphae shown in

A

Trichophyton Mentagrophytes

24
Q

Favic Chandelier/Antler Hyphae examples

A

Trichophyton Schoenleinii

25
Q

Racquest hyphae seen in

A

Epidermophyton floccosum

26
Q

Classification of Fungi on basis of location

A

Superficial Mycosis
Subcutaneous Mycosis
Deep/Systemic Mycosis

27
Q

Superficial Mycosis Causative organisms

A

Tinea versicolor
Dermatophytes

28
Q

Subcutaneous Mycosis causes

A

MRCS
Mycetoma
Rhinosporidiosis
Chromoblastomycosis
Sporotrichosis

29
Q

Deep Mycosis/systemic Mycosis causes

A

Dimorphic fungi
Opportunistic infections - Candida, Cryptococcus, Aspergillus, Mucor

30
Q

Sample taken for diagnosis of Fungal infections

A

Hair, skin, Nail
Pus
Lung - sputum, Bronchial washings, Lung biopsy
Blood
CSF
urine
Body fluids

31
Q

Chemical used for Direct examination on M/E

A

10-20% KOH Mount - digest keratin

32
Q

Stains used for diagnosis of Fungal infections

A

GMS (Gomori Methanamine Silver stain) - Black
Per-iodic acid Schiff - Pink
Gram stain
H and E stain
Calcoflour white - Fluorescent stain
Negative stain
Mucicarmine

33
Q

Fluorescent stain used for Fungus

A

Calcoflour white

34
Q

Negative stain used for diagnosis of which organism

A

India ink, Nigrosin
Used for Cryptococcal Meningitis - CSF sample

35
Q

Stain used for Diagnosis of Cryptococcal Meningitis in case of Biopsy sampe

A

Mucicarmine

36
Q

Culture used for diagnosis of Fungal infections

A

Sabouraud Dextrose agar (SDA)
Corn meal Agar
Bird seed Agar/Niger agar
Chrom agar

37
Q

Sabouraud Dextrose agar Composition and pH

A

2% Dextrose
pH - 5.6 (Acidic)
Antibiotics - Gentamicin, Chloramphenicol (kills bacteria)
Cycloheximide - to kill saprophytic fungi

38
Q

Stain used for Sabouraud Dextrose agar

A

Lactophenol cotton blue (LPCB)

39
Q

Lactophenol cotton blue composition

A

Lactic acid - Preserves Morphology
Phenol - Disinfectant
Glycerol - Prevent drying
Cotton blue - Dye
Used to study culture morphology

40
Q

Corn meal agar used to grow

A

Chlamydospores - Candida

41
Q

Cultures used for Candida

A

Corn meal agar
Chrom agar

42
Q

Bird seed agar/Niger agar used for which organism

A

Brownish color
Cryptococcus

Microbiology (37 decks)

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