Oral Microbiology Flashcards
(192 cards)
1
Q
3 features/capacities of Pathogens:
A
Host colonization
Overcome host immunity
Replicate within the host
2
Q
Name 5 virulence factors
A
Adherence Toxins Inflammatory mediators Anti-host enzymes Bacterial capsules
3
Q
What are the two types of cells
A
Prokaryotic
(includes archea)
Eukaryotic
4
Q
What is a major virulence factor in Gram negative bacteria?
A
LPS
*lipopolysaccharide
5
Q
T/F
Bacteria contain no membrane-bound organelles
A
True
6
Q
How is bacterial genetic material packaged?
A
circular dsDNA
*one molecule
7
Q
Bacterial Ribosomes ______
Eukaryotic Ribosomes ______
A
70s
80s
8
Q
What do bacteria use for attachment and conjugation?
A
Fimbrae
9
Q
What is made of carbohydrate polymers and a major virulence factor for bacteria?
A
Capsule
10
Q
Why must bacteria secrete many digestive enzymes?
A
Only small molecular weight molecules can pass through cell wall and membrane
11
Q
The ecological biosystem of bacteria are referred to as…
A
Biofilms
12
Q
T/F
There is often a battle over Fe resources between host and bacteria
A
True
13
Q
Describe the outer layers of a Gram Positive bacteria?
A
Thick wall
Single inner plasma membrane
*often 50% wall by weight
14
Q
Describe the outer layers of a Gram Negative bacteria.
A
Outer membrane (LPS)
Thin cell wall
Cell (inner) membrane
15
Q
Gram+ bacteria have ___ layers
Gram- bacteria have ____ layers
A
2
3
16
Q
What often slimy secretion do some bacteria surround themselves with that has strong anti-phagocytic properties?
A
Capsule
17
Q
What is the essential, all enclosing, rigid, porous, protective girdle that surrounds the bacterial cell?
What is its primary function?
A
Cell Wall
Protect from surrounding Osmotic Pressure
18
Q
What 2 acetylated amine groups make up the glucose dimers that make the polysaccharide of cell wall?
A
NAG - N-acetylglucosamine
NAM - N-acetylmuramic acid
19
Q
After the export of 20-ish NAG-NAM from the bacterial cell to the cell wall, what cross links the peptides and affixes the new unit to the cell wall?
A
Transpeptidases
20
Q
What constantly synthesizes and degrades the peptidoglycan making up cell wall?
A
Autolysins
21
Q
The toxic LPS found on the outer leaflet of the outer membrane of Gram- bacteria is…
A
Endotoxin
22
Q
A Gram- can’t hold its alcohol:
A
Crystal violet washes off by alcohol/acetone and accounts for lighter stain
23
Q
The pattern recognition receptor that binds to LPS is…
A
TLR-4
24
Q
TNF-alpha is a…
A
Cytokine
25
Large amounts of LPS that activate the clotting system is know as what?
Disseminated intravascular coagulation
26
What are 2 important pro-inflammatory mediators produced by Macrophage in response to LPS?
PGE-2
(osteoclast activator)
TNF-alpha
(signals NO production, causing pre-capillary sphincters to relax)
27
If TNF-alpha causes too much of an NO reaction, what could happen?
Blood pressure falls leading to
Hypovolemic Shock (aka septic shock)
28
What enzyme breaks down starch and glycogen?
Amylase
29
What enzyme breaks down peptidoglycan NAG-NAM molecules?
Lysozyme
30
T/F
| ECF matrix often has glucose polymers that make them up
True
31
Bacteria capable or respiration have what?
Heme proteins
*no heme proteins = no respiration
32
Bacteria that must have oxygen:
Bacteria that can respire if Oxygen present, use fermentation if Oxygen absent:
Not killed by Oxygen but only ferment:
Like small amounts of oxygen
Always killed by Oxygen
Obligate aerobes
Facultative
Aerotolerant
Microaerophilic
Anaerobes
33
What process gains energy by partially breaking down substrate to other organic molecules like lactic acid or alcohol?
Fermentation
34
What 2 enzymes are of utmost importance when dealing with toxic oxygen radicals?
Superoxide dismutase
Catalase
35
Superoxide dismutase:
Catalase:
radical to hydrogen peroxide
hydrogen peroxide to water and oxygen
36
Both Superoxide dismutase and Catalase are found in what type of bacteria?
Aerobes and Facultative aerobes
37
T/F
| Strict aerobes have neither Superoxide Dismutase or Catalase.
True
38
T/F
Supragingival plaque is mostly made up of microaerophilic and facultative bacteria, while sub-gingival plaque is primarily facultative and anaerobic
True
39
What inserts into bacterial DNA, can exit, and often carries resistance or toxic coding sequences?
Plasmids
40
What system acts in a fast, non-specific way that does not require specific recognition of a pathogen?
Innate
41
T/F
| Innate immunity includes inflammation
True
*however, inflammation interacts with mechanisms of adaptive
42
What system of immunity is slow and specific and remembers?
What does it use?
Adaptive
Specific B and T lymphocytes
43
What kind of cells live below all our epithelial barriers and recognize insults?
What do they release?
Sentinal
*Mast cells or Basophils
Releases Histamine
44
The "stop signs" that tell neutrophils to exit circulation in an acute condition are known as...
Tells what to exit during chronic condition?
Cell adhesion molecules
(CAMS)
Lymphocytes and Macrophage
45
S. pneumoniae is Gram...
S. pneumonia can cross BBB and initiate an inflammatory response called...
Positive
Meningitis
46
What blocks phagocytosis by PMN's and Macrophage and is a major virulence factor in S. pneumonia?
Capsule
*CHO
47
The capsule in S. pneumoniae induces what response from the immune systym?
Specifically?
Immunogenic
Opsonization with antibodies
48
What receptors on S. pneumoniae allow for opsonization?
Fc receptors
49
T/F
Cell surface receptors are utilized by cytokines (which are activated by T-cells)
Cytokines are required for a full and effective adaptive immune response
True
50
What causes the T and B cells to divide into clones?
Cytokines
*stimulated by T cells that received part of bacteria targeted from presenting cells
51
What causes a Cytokine Storm?
Super-antigen
52
What is the most biologically toxic bacterial product?
A-B toxin
53
What is the most common cold sore treatment?
Acyclovir
54
What increases the potency of vaccines?
Adjuvants
55
Hypersensitivity reactions 1-3 are...
Hypersensitivity 4 is...
Immunoglobulin mediated
T-cell mediated
56
T/F
| Gingival, sulcular, and junctional epithelium are all rapidly dividing, shedding, physical barriers.
True
57
What defines gingival junctional epithelium?
| 4 things
Poor differentiation
not much keratin
not many hydrophobic, granular materials
loosely linked
58
How do Junctional epithelial cells activate T-helper cells?
with HLA class II
*present extracellular antigenic peptides
59
Junctional epithelial cells have numerous cytokines and chemokines. What one is found in large amounts?
Chemokine IL-8
60
Cytokines and chemokines are _____ for PMN's
chemotactic
61
How is junctional epithelium different from sulcular/gingival?
Junctional epithelium allows for easy _____.
Non-shedding
Egress of PMN's
62
What is the exception to epithelia of the mouth being tough, highly keratinized, and mostly impermeable?
What does this allow?
Junctional Epithelium
*epithelium that attaches to the tooth
PMN's easy access to the mouth
63
Outside of the cervicular space, what is a major protective barrier?
Saliva
*sIgA
64
2/3 of the WBC's are..
PMN
*neutrophils
65
Name two cell surface receptors PMN's use to attach foreign microorganisms.
Fc
C3b
66
PMN's either kill pathogens by ingesting them, or ____
drooling
*this leads to many side-effects of inflammation
67
Salivary glands are Ig__ dominant and inflamed gingiva (Crevicular fluid) is Ig__ dominant
IgA
IgG
68
What two sources of fluid bathe the oral cavity?
Crevicular fluid
Saliva
69
What are the 2 major defense features of Crevicular fluid?
IgG
PMN's
70
What is a big anti-carie effect of saliva?
Buffers
71
Dimeric IgA cells from B cells are cleaved at what site?
When released to mucosa the complex at site is called?
Fc
Secretory component
72
What are 4 unique attributes of sIgA?
Sticks to mucins
Protease resistant
Neutralizes viruses/toxins
Blocks microbe colonization
73
Name 3 supragingival plaque film Gram+ bacteria associated with Caries.
Streptococcus
Lactobacillus
Actinomyces
74
Name 5 Gram- facultative and anaerobic rods bacteria associated with Periodontal Disease
```
Treponema
Aggregatibacter
Bacteroides
Tannerella
Porphyromonas/Prevotella
```
75
How many different species of bacteria call the mouth home?
800
76
What has been shown to inhabit some perio pockets?
Archea
77
Where do most bacteria live in the mouth
| (what 2 places)?
Plaque
Tongue crypts
78
Supragingival plaque biofilm is primarily made of _______ and _______ bacteria.
Facultative
Microaerophilic
79
______ and ______ make up most of the subgingival plaque biofilm
Facultative
Anaerobic
80
T/F
| Gingivitis usually has both G+ and G- associated with it.
True
81
Describe Streptococcus Viridans
| 7 features
```
Alpha Hemolytic
Green on blood agar
Facultative
ECM producer
saccharolytic
Acidogenic
```
82
What are the 4 subgroups of Streptococcus Viridans?
Which are good/bad?
S. anginosus
S. mutans - the bad guys
S. mitis
S. salivarius
83
Name 2 species in the Streptococcus mutans subgroup
Mutans
Sobrinus
*these 2 species most often associated with dental plaque
84
What Strep. species is a major cause of caries?
Name 3 features of this species
Mutans
insoluble polysaccharides
prefers low pH
Lactic Acid producer
85
What S. Viridans subgroup is found in plaque, mucosa, and respiratory tract and is pus forming?
S. anginosus
86
What S. Viridans subgroup is the pioneer species, making insoluble and soluble polysaccharides, and associated with bacterial endocarditis?
S. mitis
87
Name 4 species in the S. mitis group
S. sanguinis
S. gornonii
S. oralis
S. mitis
88
What S. Viridans subgroup colonizes mucosa and not plaque?
| is a good guy
S. salivarius
89
Name 2 species in the S. salivarius subgroup of S. Viridans.
S. salivarius
| S. vestibularis
90
T/F
| Peptostreptococcus is Anaerobic
True
91
What bacterial species is an opportunist that causes nosocomial problems and can survive harsh environments?
Enterococcus
92
What species of Enterococcus is commonly implicated in endodontic root canal failures and persistent infections
E. faecalis
93
Streptococcus are all Gram___ cocci arranged in ____
Positive
Chains
94
Subacute Bacterial Endocarditis is often caused by what?
| also pneumonia and caries
Viridans Strep
95
What type of bacteria is associated with Root caries, ECM production, eats sugars, and tolerates a low pH?
Name a specific species
Actinomyces
A. israelii
96
What species of bacteria is correlated with a high-sugar cariogenic diet, dentin caries, and is the Advancing Front of caries lesions?
(also produces ECM, eats sugar, tolerates low pH)
Lactobacillus acidophilus
97
What 2 bacteria can metabolize lactate (and theoretically reduce caries)?
Neisseria
Veillonella
98
Name 4 Oral G+ rods.
Actinomyces
Lactobacillus
Neisseria
Veillonella
99
What G- rod is associated with aggressive Periodontitis in young adults?
What kind of toxin does it produce?
Aggregatibacter actinomycetemcomitans
Leukotoxin
100
What G- rod associated with Periodontitis has very long thin rod that other bacteria attach to?
Fusobacterium nucleatum
101
Name 4 black pigmenting species of G- rods that (might be) causative agents in Periodontal Disease
Bacteroides
Tanerella
Porphyromonas
Prevotella
102
What 3 species form the Red Complex associated with aggressive Perio infections?
T. forsythensis (Tannerella)
P. gingivalis (Porphyromonas)
T. denticola (Treponema)
103
Name an Asachrolytic G- rod.
Porphyromonas gingivalis
104
What species of Prevotella is associated with periodontitis?
What type (G)
*all Prevotella sacharolytic
P. intermedia
G-
105
What very anaerobic species is found in gingivitis and periodontitis?
Treponema denticola
106
2 shapes of viral capsid
Icosahedral
| helical
107
What are the 2 ways a newly replicated virus leaves the cell
Budding (envelope with spikes)
Lysis
*enveloped vs. naked
108
5 cellular outcomes of viral infection
```
Abortive (no progeny)
Lysis
Chronic non-lytic
Latent (integration)
Transformation (cell proliferates uncontrollably)
```
109
4 binary viral classifications:
helical or icosahedral
enveloped or naked
DNA or RNA
ds or ss
110
The protein shell of a virus is called a...
Capsid
111
What 2 things must a virus do to replicate within the cell?
Replicate their genetic material
Produce +mRNA for protein components
112
What plant infecting nucleic acids lack capsids?
Viroids
113
Horizontal infection:
Vertical infection:
person to person
mother to fetus/neonate
114
No symptoms can lead to a large ______
Iceberg effect
115
Herpes viruses are enveloped/naked?
ds/ss?
DNA/RNA?
Enveloped
dsDNA (large - many targets)
116
Where do herpes viruses replicate and assemble?
nucleus
117
Herpes virus acquires their membrane before/after they reach the cytoplasmic membrane.
Before
***this is unique
118
Herpes initially infects the _______ and lasts _____
mucosal epithelium
lifetime
119
Example of an antiviral therapy for Herpes:
Acyclovir
120
T/F
| Herpes is serious if fetus infected, mild if child, and moderate to serious in adults
True
121
HHV 1-4:
1: cold sores
2: genital
3: Varicella Zoster (chickenpox, shingles)
4: EBV
122
What HHV strains are latent in the neuron?
1-3
123
What HHV is an important congenital infection?
Cytomegalovirus
124
What Herpes virus is Kaposi's sarcoma?
HHV-8
125
DNA viruses can cause...
Tumors
126
What HHV isn't spread through mucosal contact?
Varicella zoster (3)
127
Viremic (first) infections can be dangerous to...
Fetus
128
What is the name for the manifestation of HSV-1?
Herpetic Stomatitis
129
What is the most common cause of viral encephalitis in the USA?
HSV-1
130
Describe Herpetic Stomatitis
small vesicles
clear liquid
rupture and leave small painful ulcer
131
What is the leading infectious cause of blindness in the USA?
HSV-1 keratitis
132
HIV transmission is increased 2-4 fold with the presence of what?
HSV-2
133
In the 1st 5 years of life HSV-1 primarily presents with a lip lesion, but is ____ asymptomatic
85%
134
Lesions of HSV-1 present in what 2 places?
| area called what
gingivostomatitis
herpes labialis
135
What ganglia are typically infected in HSV-1?
HSV-2?
Trigeminal
Sacral
136
T/F
Primary HSV-1 and HSV-2 infections can pass to fetus
Secondary recurrences of HSV-2 can pass to fetus during delivery
True
137
The only HHV spread by respiratory droplets is
| infects lung mucosal lining initially
Varicella Zoster
*zoster is the shingles bit
138
What are the 2 major diseases caused by EBV?
Infections mononucleosis
Burkitt's B Cell Lymphoma
139
Burkitt's lymphoma, aside from having an association with EBV, is associated with...
Malaria
140
Hairy Leukoplakia is caused by what virus?
*benign, which lesion, hyperkeratosis
EBV
141
2 cancers associated with EBV
Burkitt's lymphoma
Nasopharyngeal Carcinoma
142
T-cell leukemia is associated with what virus?
HTLV-1
143
What percentage of young adults infected with EBV develop Mono?
20-50%
144
What is the number 1 cause of retardation?
Cytomegalovirus primary infection when pregnant
145
T/F
| Acyclovir works on Cytomegalovirus
False
146
T/F
| There is no vaccine for the multiple strains of Cytomegalovirus
True
147
Worldwide ____% seropositive for cytomegalovirus
| US ____ %
100% ww
50% US
148
Of the 1% pregnant that develop primary Cyto infections, ___% develop neural sequelae in fetus
20%
149
CMV is the most common cause of intrauterine infections and congenital abnormalities in the US
True
150
Primary or reactivation of latent CMV is very serious in what population?
Immunosuppressed
151
Describe the Coxsackie virus
ssRNA
non-enveloped
picornaviridae
common and mild diseases in young
152
What is the non-specific flu-like illness caused by the Coxsackie virus?
Herpangina
153
What 2 diseases does the Coxsackie virus cause?
Herpangina
Hand, foot and mouth disease
*both mostly young children
154
What is the most communicable disease worldwide (no iceberg effect)?
Measles
155
Describe the measles virus (capsid, genome)
Enveloped
ssRNA
156
Describe the Mumps virus (capsid, genome)
Enveloped
ssRNA
157
The characteristic symptom for mumps is...
Inflammation associated with this does what?
Parotitis
blocks salivary fluids
158
HPV - describe (capsid, genome)
non-enveloped
dsDNA
*warts - hyperplastic epithelial lesions
159
HPV is associated with what type of cancer?
Cervical
160
What is the most common fungal infection relevant to dentistry?
Candida albicans
161
Candidiasis is an _________.
Opportunistic bloom
162
What is an organism that lives on dead/decaying matter?
Saprobe
163
What is different about a fungal cell membrane?
| compared with human cell mem
Ergostal instead of cholesterol
164
What inhibits ergostol synthesis?
Azoles
165
What drug binds to and forms holes in ergostol-containing membranes?
polyenes
166
What makes up the cell wall of fungi?
cross-linked glucans
*Chitin
167
T/F
| Compared to antibiotics, there are relatively few anti-fungals.
True
*Because non-toxic anti-eukaryotic drugs hard to find
168
2 basic morphologies of fungus:
Yeast
Hyphae/filamentous
169
The collective term for a mass of hyphae is...
mycelium
170
Some yeast are ______ , and can exist in both yeast and filamentous forms
Dimorphic
171
What fungus of oral concern can assume a pseudohyphae form and is dimorphic/polymorphic
C. albicans
172
T/F
| Fungi has a huge cell wall, up to 90% of dry weight
True
173
Why is humoral immunity no good in combating fungi?
Proteases/enzymes of fungi
174
T/F
| Fatty acids, pH, epithelial cell turnover, and normal bactera all help resist against fungal invasions
True
175
Mycotoxicoses are _____
rare
176
Hay fever and asthma is a Type ____ hypersensitivity
Contact Dermatitis to a fungal product is a Type ____ hypersensitivity
Type I
Type IV
177
T/F
| Superficial mycoses typically don't cause pathology
True
178
______, think cutaneous mycoses
Tinea
179
What 3 Dermatophytes cause Tinea (cutaneous mycoses)?
Trichophyton
Micosporum
Epidermophyton
180
T/F
| Subcutaneous mycoses are common
False
181
Systemic fungal infections tend to enter via what?
Lungs
| inhaled spores
182
List 4 opportunistic mycoses from common to rare:
Candidiasis
Cryptococcus
Aspergillosis
Pneumocystis
183
T/F
| Candida albicans, along with being an opportunist, is part of the normal flora
True
80%
184
What should a clinician think in an otherwise healthy adult in case of oral thrush?
Immuno-compromising conditions
| HIV
185
The yeast or hyphae form of Candida depends on what?
Acidity
* acidic favors yeast, alkaline favors hyphae
* *remember it's pseudohyphae for Candida
* **Polymorphic
186
A pH switch to alkaline promotes what kind of Candida?
Filamentous
187
Why doesn't humoral immunity do much against fungi?
Antibodies are degraded quickly by enzymes / proteases
188
What type of cells typically respond to fungal antigens?
How?
Th1
| stimulate cytokines
which initiates inflammation, cell turnover, etc
189
T/F
| An immune dysfunction will almost always have thrush
True
190
PMN's attacking Candida can sometimes form what?
Loosely adherent pseudomembranes
* easily wiped off
* *diptheria firmly adhere
191
Primary Candidiasis is found where?
Secondary Candidiasis is found where?
Which is seen in immunosuppression and HIV?
Orally (and surrounding)
Other mucosal/cutaneous sites
Secondary
192
4 types of Primary Oral Candidiasis:
Acute Pseudomembranous
Erythematous (acute/chronic atrophic)
Chronic hyperplastic (leukoplakia EBV associated)
Chronic mucocutaneous (immune defect)
