7.3.2 Polymerase Chain Reaction: DNA Amplification

Question 1

During PCR, DNA is denatured by __________, while the binding of primers is encouraged by __________.

Answer 1

• heating, cooling

Question 2

If PCR was started with a single piece of double-stranded DNA, how many copies of the DNA fragment would there be after 3 cycles?

Answer 2

• 8

Question 3

Which of the following is not true about PCR?

Answer 3

• It requires reverse transcriptase

Question 4

PCR requires which starting materials?

i. DNA to be copied
ii. DNA polymerase
iii. Nucleotides
iv. DNA primers

Answer 4

• i, ii, iii, iv

Question 5

Polymerase chain reaction (PCR)

Answer 5

• is a method of making copies of a specific segment of DNA from a single piece of double-stranded DNA.
• is a method of making copies of a specific segment of DNA from a single piece of single-stranded DNA.