Cell Bio: Cytoskeleton and Cytoplasm Flashcards Preview

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Flashcards in Cell Bio: Cytoskeleton and Cytoplasm Deck (21):
1

Cytoskeletal filaments from smallest to largest

1. microfilaments
2. intermediate filaments
3. microtubules

2

Two components that make up mictrotubules

Alpha and beta tubulin

3

B tubule is oriented towards + or - end of MT?

+ end, one distal to nucleus

4

Dynein moves which way

retrograde (from + to - ends)

5

Kinesin moves which way

anterograde ( - to + end)

6

Cilia are important in what three places in body

1. respiratory tract
2. paranasal sinuses
3. fallopian tubes

7

What structure links peripheral 9 doublets in the 9+2 arrangement of microtubules in a cilia?

Axonemal dynein (ATPase that causes bending of cilia)

8

What disease results from axonemal dynein arm defect?

Primary ciliary dyskinesis, aka Kartagener's Syndrome

9

Main 4 issues in Kartagener's syndrome?

1. bronchiectasis
2. chronic sinusitis
3. infertility
4. situs inversus (50% of cases)
*bronchiectasis + chronic sinusitis + situs inversus is pretty diagnostic; situs inversus b/c cilia important in organ placement during development

10

What intermediate filament is present in connective tissue ( fibroblasts, leukocytes, endothelium) that can be stained for? What CA can the stain be useful in detecting?

Vimentin--> stains can help ID sarcomas

11

What int. filament is found in muscle cells? Stains can help ID what CA?

Desmin--> myosarcomas (rhabdomyosarcoma, leiomyosarcomas, etc.)

12

What int. filament can be stained for in epithelial cells? What CA?

Cytokeratin--> carcinomas (cancers of epithelial cell origin)

13

What int. filament is found in glial cells? CA stains?

GFAP (glial fibrillary acid proteins)--> astrocytomas (glioblastoma)

14

What int. filament is found in neurons?

Peripherin

15

What int. filament is found specifically in the axons within neurons? CA?

Neurofilaments--> neuroblastomas (in adrenal medulla in kids)

16

*What int. filaments are found in the nuclear envelope and the DNA within?

Laminins (A, B, C types)

17

What two dz can result form mutated laminins?

1. Progeria (early aging of cells, wrinkly skin on 5 y.o.)
2. Muscular dystrophy

18

What structures allow for coordinatd movement of cilia on different cells?

Gap junctions

19

What are 5 drugs/ drug classes that interfere with microtubule function?

1. Vinca alkyloids --> Vincristine, Vinblastine
2. Taxanes --> Paclitaxel, Docetaxel
3. Benzimidazoles --> Mebendazole, Albendazole, Thio-
4. Griseofulvin--> antifungal
5. Colchicine

20

What disease results from impaired microtubule-dependent sorting of endosomal proteins in late multivesicular endosomes?

Chediak-Higashi syndrome--> due to mutation in lysosomal trafficking regulator gene (LYST); recurrent pyogenic infections, partial albinism, peripheral neuropathies

21

3 lysosomal storage dz

1. Gaucher
2. Niemann-Pick
3. Tay-Sachs

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