DNAligase Flashcards

1
Q

​Como existem 2 tipos de extremidades geradas pelas ER:

A

→ existem 2 tipos de ligase.

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2
Q

DNAligase - Tipos

A

DNAligase

T4 DNAligase

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3
Q

Micro-organismos - DNAligase → ?

Micro-organismos - T4 DNAligase → ?

A

Micro-organismos - DNAligase → E. coli

Micro-organismos - T4 DNAligase → Bacteriófagos T4

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4
Q

DNAligase - E. coli - Função

A

Ligam fita simples de DNA (dentadas, coesivas ou adesivas).

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5
Q

T4 DNAligase - Bacteriófagos T4 - Função

A

Ligam fita dupla de DNA (abruptas ou cegas).

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6
Q

Ligase de E. coli → ?

Ligase de Bacteriófagos → ?

A

Ligase de E. coli → não consegue ligar as abruptas / cegas.

Ligase de Bacteriófagos → conseguem ligas as fitas abruptas / cegas.

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7
Q

T4 DNAligase → Preço

A

+ cara.

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