Flashcards in 3.6 Enzymes Deck (20)
What is an enzyme?
An ENZYME is a protein that acts as a catalyst in a living things
How does an enzyme work?
An enzyme works by LOWERING the ACTIVATION ENERGY of a chemical reaction
How do you name an enzyme?
replace ending at end of substrate with ase
What are the 3 characteristics of exergonic reactions?
What are the 3 characteristics of endergonic reactions?
What happens to enzymatic speed with substrate concentration and why?
Enzyme activity increases as substrate concentration increases because there are more collisions between substrate molecules and the enzyme.
What happens to enzymatic speed with temperature and why?
As the temperature rises, enzyme activity increases because more collisions occur between enzyme and substrate.
If the temperature is too high, enzyme activity levels out and then declines rapidly because the enzyme is denatured.
What happens to enzymatic speed with pH and why?
Each enzyme has an optimal pH at which the rate of reaction is highest.
What is inhibition?
active enzyme is prevented from combining with substrate.
What is competitive inhibition?
Blocks substrates from entering the active site
What is noncompetitive inhibition?
Change formation of enzyme by binding to another site
What is feedback inhibition?
The end product of a reaction blocks its own production
How does penicillin work?
Block an enzyme that makes the cell wall in bacteria
How does aspirin work?
Blocks the enzyme cyclooxygenase
How does prozac work?
Inhibits the reuptake of seratonin
How does viagra work?
binds to the receptors of the enzyme guanylate cyclase-leads to increased blood flow.
What are the 4 characteristics of the enzyme-substrate?
Active site and substrate complement each other in terms of both shape and chemical properties (e.g. opposite charges)
Binding to the active site brings the substrate into close physical proximity, creating an enzyme-substrate complex
The enzyme catalyses the conversion of the substrate into a product (or products), creating an enzyme-product complex
As the enzyme is not consumed in the reaction, it can continue to work once the product dissociates (hence only low concentrations are needed)
What is denaturation?
Denaturation is a structural change in a protein that results in the loss (usually permanent) of its biological properties
How can lactose free milk be made?
Lactose-free milk can be produced by purifying lactase (e.g. from yeast or bacteria) and binding it to an inert substance (such as alginate beads)