DAT Final Random info Flashcards

1
Q

Prokaryotic cells can further be
categorized into two domains:

A

Bacteria
b. Archaea

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2
Q

Prokaryotic genomes are…

A

similar in size

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3
Q

eukaryotic genomes are…

A

typically larger in size

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4
Q

bacterial cells wall

A

composed of peptidoglycan, a
carbohydrate and protein polymer that
encompasses the whole bacterial cell and
allows for anchoring

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5
Q

RNA polymerase bacteria

A

1 single type of RNA poly

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6
Q

do bacteria contain histones or chromatin

A

no

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7
Q

Pfu polymerase

A

polymerase
found in thermophile archaeans that
is used in PCR because of its
stability and accuracy in high
temperatures

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8
Q

Methanogens

A

archaeal organisms that
release methane as a byproduct.

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9
Q

Cell wall archaea

A

archaea cell walls do not
contain peptidoglycan

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10
Q

Histones

A

positively charged, basic
proteins that provide structural support to
help organize and condense negatively
charged DNA

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11
Q

bond between positively charged
histones and negatively charged DNA

A

ionic bond

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12
Q

Transcriptome

A

the entire set of
mRNA expressed by a cell

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13
Q

Proteome

A
  • the entire set of proteins
    expressed by a cell
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14
Q

Exons

A

coding DNA regions for
polypeptides

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15
Q

Introns

A
  • non-coding regions in
    DNA that lay within gene sequences,
    between exons
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16
Q

Regulatory sequences

A

control
the expression of genes by serving as
binding sites for regulatory proteins

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17
Q

Telomeres

A

repetitive DNA
sequences the ends chromosomes
that prevent the loss of genetic
information during multiple cycles
of cell replication

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18
Q

Transposable elements

A

stretches of DNA that can move from
one region of the genome to another

19
Q

Retrotransposons

A

move with the
copy and paste method via an RNA
intermediate

20
Q

DNA methylation

A

addition of
methyl (-CH3) groups to the
nitrogenous bases on a DNA strand.
DNA methylation results in decreased
gene expression

21
Q

Histone acetylation

A

addition of
an acetyl (-CH3CO) group to an
amino acid (lysine) found in the
histone tails that protrude from the
histone core

22
Q

Prader-Willi Syndrome

A

deletion or mutation of the paternal
copies of the genes. (chromosome 15)

23
Q

Angelman Syndrome

A

copies of these genes inherited
from the mother are expressed, while
the copies on the paternal chromosome
are imprinted (silenced/not expressed). (chromosome 15)

24
Q

Pattern I development

A

males during colder temperatures
and females in warmer
temperatures (turtles)

25
Pattern II development
female offspring during low and high temperatures, but male offspring during intermediate temperatures
26
Capsid
surrounding the nucleic acids of the virus is a protein coat called the capsid which protects the viral genome
27
Capsomeres
smaller protein units that the capsid is composed of
28
Viral envelope
membranous outer layer that can improve the ability of a virus to infect its host
29
structure of a phage
nucleic acid, capsid head, protein tail
30
lytic cycle
phage infects and then actively replicates in the host cell before lysing the host to release viral progeny.
31
lysogenic cycle
the phage does not actively create new copies of itself. Instead it integrates its genome into the host genome and continues to have its genome replicated when the host cell divides.
32
Prions
mis-folded proteins that are infectious and cause other proteins to mis-fold.
33
Viroids
simple molecules of circular single-stranded RNA that infect plants
34
RNA polymerase I
i. Located in the nucleolus ii. Synthesizes the majority of rRNAs. a. Note, rRNA molecules are described as being structural RNAs. This is because they are not translated into proteins, but they do perform cellular roles
35
RNA polymerase II
located in nucleus ii. Responsible for transcribing most eukaryotic genes iii. Synthesizes ALL protein coding pre-mRNAs a. Note that pre-mRNA must be processed to mRNA after transcription, but prior to translation
36
RNA polymerase III
i. Located in the nucleus ii. Transcribes various structural RNA molecules, such as small nuclear pre-RNAs and pre-tRNAs (pre- transfer RNAs)
37
Promoter:
A segment of DNA where RNA polymerase binds in order to initiate transcription.
38
“Downstream”
describes the direction of transcription.
39
“Upstream”
describes the direction opposite of transcription.
40
siRNAs
similar to miRNAs in terms of how they are produced, however, siRNAs are formed from long, double-stranded RNA molecules as opposed to single- stranded RNA molecules in miRNA.
41
tRNA molecules
function to “translate” the genetic message that was written in the language of mRNA into a protein’s native language.
42
Bound Ribosomes:
either bound to the cytosolic side of the rough endoplasmic reticulum or at the nuclear envelope.
43
Bound Ribosomes function
synthesizing proteins that become part of the endomembrane system.
44
Free Ribosomes function
Create cytosolic proteins that remain functioning in the cytosol.