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Principles of disease (Ross Lilley) > Introduction to microbiology > Flashcards

Flashcards in Introduction to microbiology Deck (15)
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1

categories of micro-organisms that cause infection

-bacteria
-viruses
-fungi
-prions
-parasites

2

common specimen types collected for culture, sterile sites.

-Blood
-CSF
-Lung
-Bladder

3

common specimen types collected for culture, non-sterile sites.

-Skin
-Nasopharynx
-Urethra
-Gut

4

diagnosis of bacterial infections using microscopy

Light microscopy can be used, stain varies:
-no stain to see pus cells or parasites
-Gram stain to see bacteria and yeast/fungi
-ZN or Auramine stain for mycobactria

5

Pros and cons of gram stain microscopy for bacterial identification.

pros: rapid
cons: not sensitive, can't usually identify particular species

6

Pros and cons of bacterial culture for identification.

pros: sensitive
cons: slow

7

selective bacterial growth media

only grows a specific bacteria or doesn't grow some species of bacteria

8

non-selective bacterial growth media

grows all bacteria

9

methods for identification of bacterial species

use observable characteristics:
-morphological (shape) using miscroscopy
-physiological (normal functions of the bacteria)
-biochemical (chemicals within and excreted by cells)

DNA based tests

10

sterile site

site in body that should normally not contain microorgansims

11

non-sterile site

site in the body that normally contains micro-organisms

12

Methods for detecting viruses

-molecular methods, real time PCR
-antigen detection
-serology to determine immunity

13

diagnostic principles in parasitology

-microscopy, looking for parasites, cysts and ova in faeces. Blood films for malaria.
-culture rarely possible
-serology sometimes useful

14

basic infection control measures

-wash hands before and after patient contact aswell as contact with patient's immediate environment.
-swab stethoscope between patients
-isolation of patients, barrier nursing or source isolation

15

prions

infectious agents composed entirely of protein