Lec 1 - 3 - Bacteriophages Flashcards Preview

Dental Microbiology > Lec 1 - 3 - Bacteriophages > Flashcards

Flashcards in Lec 1 - 3 - Bacteriophages Deck (42):
1

bacteriophages are the basis for blank technologies

recombinant dna (T4 ligase, T7 polymerase, cloning etc)

2

this alters bacterial virulence properties

lysogenic conversion

3

many microbes are harmless because they cant synthesize their blank

toxin

4

size of t2 bacteriphage

65 nm

5

size of hemoglobin molecule

15 nm

6

each phage attaches to one blank cell

bacteria cell

7

t phages and lambda are blank nucleic acid composition

double stranded dna

8

linear phage for sequencing used in cloning

m13 (ssDNA)

9

primary objective of phages is blank

replication

10

steps for a t4 phage life (lytic)

adsorption, penetration, replication

11

bacteriophages can be either blank or blank

lytic, temperate

12

t4 binds to blank during adsorption and then blank is made

e coli, LPS

13

the "legs" of the bacteriophage that binds and finds a cell to bind to

fembrils

14

steps of phage morphogenesis

preferential mRNA synthesis  DNA synthesis  protein synthesis  incomplete head assembly  DNA packaging  tail addition  maturation  lysis of cell

15

period of time from immediate point of absorption to phage release

latent period

16

absorption to mature phage particles produced

eclipse period

17

temperate bacteriophage is aka blank

lysogenic

18

temperate bacteriophage actually blank itself into the genome or is floating in cytoplasm

incorporates

19

when conditions become adverse, lysogenic bacteria become blank

lytic

20

the model organism for a temperate bacterophage

bacteriophage lambda

21

lambda dna goes from linear to circular via blank

COS sites

22

sticky ends of the lambda dna is blank rich ssDNA

GC

23

the sticky ends of lambda dna are blank of each other

complements

24

sticky ends are gc rich because they have blank so they are stronger than AT

3 H bonds

25

four promoters that decide whether lytic or lysogenic

PL, PR, PRE, PRM

26

promoter in charge of RNAP for transcribing genes for lysis on left

PL

27

promoter in charge of RNAP for transcribing genes for lysis on right

PR

28

repressor of PL and PR transcription but activates PRM

cI

29

protein that is an inhibitor of cI; anti repressor

Cro

30

lysogenic cycle is from blank activation

PRE

31

lytic cycle is from blank activation

PR

32

protein transcribed in lysogenic cycle

cI

33

protein transcribed in lytic cycle

CRO

34

three factors of lysogeny

attP, attB, Int

35

attachment phage site on phage

attP

36

attachment bacterium site

attB

37

phage integrase protein

Int

38

attP and attB are both recognized by blank whch then recombines them into attBP

Int

39

lambda has developed a blank immunity

superinfection

40

lytic infection is blocked by pre existing blank

cI

41

lysogenic infection blocked by lack of blank sites because they have all been transformed to attBP

attB

42

lambda jumps ship from the lysogenic phase to become lytic via

excision which is the opposite of integration