Lec 1 - 3 - Bacteriophages

Question 1

bacteriophages are the basis for blank technologies

Answer 1

recombinant dna (T4 ligase, T7 polymerase, cloning etc)

Question 2

this alters bacterial virulence properties

Answer 2

lysogenic conversion

Question 3

many microbes are harmless because they cant synthesize their blank

Answer 3

toxin

Question 4

size of t2 bacteriphage

Answer 4

65 nm

Question 5

size of hemoglobin molecule

Answer 5

15 nm

Question 6

each phage attaches to one blank cell

Answer 6

bacteria cell

Question 7

t phages and lambda are blank nucleic acid composition

Answer 7

double stranded dna

Question 8

linear phage for sequencing used in cloning

Answer 8

m13 (ssDNA)

Question 9

primary objective of phages is blank

Answer 9

replication

Question 10

steps for a t4 phage life (lytic)

Answer 10

adsorption, penetration, replication

Question 11

bacteriophages can be either blank or blank

Answer 11

lytic, temperate

Question 12

t4 binds to blank during adsorption and then blank is made

Answer 12

e coli, LPS

Question 13

the “legs” of the bacteriophage that binds and finds a cell to bind to

Answer 13

fembrils

Question 14

steps of phage morphogenesis

Answer 14

preferential mRNA synthesis  DNA synthesis  protein synthesis  incomplete head assembly  DNA packaging  tail addition  maturation  lysis of cell

Question 15

period of time from immediate point of absorption to phage release

Answer 15

latent period

Question 16

absorption to mature phage particles produced

Answer 16

eclipse period

Question 17

temperate bacteriophage is aka blank

Answer 17

lysogenic

Question 18

temperate bacteriophage actually blank itself into the genome or is floating in cytoplasm

Answer 18

incorporates

Question 19

when conditions become adverse, lysogenic bacteria become blank

Answer 19

lytic

Question 20

the model organism for a temperate bacterophage

Answer 20

bacteriophage lambda

Question 21

lambda dna goes from linear to circular via blank

Answer 21

COS sites

Question 22

sticky ends of the lambda dna is blank rich ssDNA

Answer 22

GC

Question 23

the sticky ends of lambda dna are blank of each other

Answer 23

complements

Question 24

sticky ends are gc rich because they have blank so they are stronger than AT

Answer 24

3 H bonds

Question 25

four promoters that decide whether lytic or lysogenic

Answer 25

PL, PR, PRE, PRM

Question 26

promoter in charge of RNAP for transcribing genes for lysis on left

Answer 26

PL

Question 27

promoter in charge of RNAP for transcribing genes for lysis on right

Answer 27

PR

Question 28

repressor of PL and PR transcription but activates PRM

Answer 28

cI

Question 29

protein that is an inhibitor of cI; anti repressor

Answer 29

Cro

Question 30

lysogenic cycle is from blank activation

Answer 30

PRE

Question 31

lytic cycle is from blank activation

Answer 31

PR

Question 32

protein transcribed in lysogenic cycle

Answer 32

cI

Question 33

protein transcribed in lytic cycle

Answer 33

CRO

Question 34

three factors of lysogeny

Answer 34

attP, attB, Int

Question 35

attachment phage site on phage

Answer 35

attP

Question 36

attachment bacterium site

Answer 36

attB

Question 37

phage integrase protein

Answer 37

Int

Question 38

attP and attB are both recognized by blank whch then recombines them into attBP

Answer 38

Int

Question 39

lambda has developed a blank immunity

Answer 39

superinfection

Question 40

lytic infection is blocked by pre existing blank

Answer 40

cI

Question 41

lysogenic infection blocked by lack of blank sites because they have all been transformed to attBP

Answer 41

attB

Question 42

lambda jumps ship from the lysogenic phase to become lytic via

Answer 42

excision which is the opposite of integration