Principles of Bacterial Isolation and Identification Flashcards Preview

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Flashcards in Principles of Bacterial Isolation and Identification Deck (12):
1

What are PIA plates?

Pseudomonas isolation agars, used to isolate pseudomonas organisms

2

Describe HEK plates and their purpose.

HEK plates are Hektoen enteric agars that contain lactose, sucrose, salicin, and peptone. Most bacteria use the three sugars, which turn the plate yellow or red, but shigella and salmonella cannot; instead, they utilize the peptone, which alkalizes the mixture and turns it blue.

3

Salmonella reduces _________, which turns a HEK plate black.

H2S

4

Mackonkey agars are used to _________.

isolate Gram-negative bacteria (because the crystal violet prevents growth of Gram-positive bacteria) and to differentiate enteric bacteria; enteric bacteria can metabolize lactose, which will turn the pH indicator red

5

Bacillus cereus is ________.

Gram-positive, rod-shaped

6

Staphylococcus aureus is _________.

Gram-positive, coccal

7

Escherichia coli is _________.

Gram-negative, rod-shaped

8

Pseudomonas aeruginosa is ________.

Gram-negative, rod-shaped

9

Klebsiella pneumoniae is _______.

Gram-negative, rod-shaped

10

Proteus mirabillis is ___________.

Gram-negative, rod-shaped

11

Salmonella is ____________.

Gram-negative, rod-shaped

12

Enterococcus is ____________.

Gram-positive coccal

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