1.1.5 Basic Principles and Techniques (Reference interval studies, Method Evaluation) Flashcards
Reference interval derives from?
a. Confidence interval
b. Sensitivity
c. Precision
d. Accuracy
a
Done to confirm the validity of an existing or published Reference interval for an analyte
a. Verifying a reference interval
b. Establishing a reference interval
a
Also known as Transference study
a. Verifying a reference interval
b. Establishing a reference interval
a
In Verifying a reference interval, It requires at least ______ study individuals and the Reference interval is adopted if ___________ of the subjects fall outside the range
20
<10%
In Establishing a reference interval, requires at least _______ study individuals and the reference interval is set base on the ______________
120
Confidence interval = mean +_ 2s
In establishing a reference interval, n=120
Mean = 5.0 mmol/L
s = 0.5
What is the reference interval?
4-6 mmol/L
Solution:
0.5 x 2 = 1
5 - 1 = 4
5 + 1 = 6
= 4 - 6 mmol/L
First step in method evaluation; usually done by running two control materials twice a day over 10 day period (2x2x10)
a. Precision study
b. Recovery study
a
Invoves spiking a sample with a known amount of a analyte and determining how much of it can be detected by the method in the presence of other compounds in the matrix
a. Precision study
b. Recovery study
b
In t-test, it compares the ________ of two groups of data or the ________ of two methods
mean
Accuracy
F test compares the ________ of two groups of data or the _________ of two procedures
Standard deviation
Precision
Used to compare two methods using the best fit line through the data points
Linear regression
Linear regression
x= ________________
Independent variable
(Reference method)
Linear regression
y= ________________
Dependent variable
(New method)
ability of a method to detect only the analyte of interest
a. Analytical sensitivity
b. Analytical Specificity
b
Ability of a method to detect the smallest concentration of an analyte
a. Analytical sensitivity
b. Analytical Specificity
a