3.2.2.2 Clinical Enzymology (Lactate Dehydrogenase, LD determination) Flashcards
LDH specimen consideration:
- Avoid hemolysis
- Avoid cold storage
- Avoid chelating agent
Which of the following does not belong?
None
LDH is the most affected enzyme by hemolysis
T or F
T
Which among the isoenzymes of LDH is the most cold-labile
a. LD1
b. LD2
c. LD3
d. LD5
d
Forward
a. Wacker
b. Wroblewski-Ladue
a
reverse reaction
a. Wacker
b. Wroblewski-Ladue
b
Lactate + NAD -(LDH)-> Pyruvate + NADH + H
a. Wacker
b. Wroblewski-Ladue
a
Pyruvate + NADH + H -(LDH)-> Lactate + NAD
a. Wacker
b. Wroblewski-Ladue
b
Reference value for LDH
a. 200-250 U/L
b. 200-225 U/L
c. 100-200 U/L
d. 100-225 U/L
d
MI Protein Markers:
a. Myoglobin
b. Troponin I
c. Fetoprotein
d. Cardioprotein
a and b
MI Markers:
Sequence of elevation
a. Myo
b. Tn (i)
c. CK
d. AST
e. LD
a,b,c,d,e
(MICAL)
MI Markers:
Elevation: 1-3hrs
Peaks: 5-12 hrs
a. Myo
b. Tn (i)
c. CK
d. AST
e. LD
a
MI Markers:
Elevation: 12-24hrs
Peaks: 48-72 hrs
a. Myo
b. Tn (i)
c. CK
d. AST
e. LD
e
MI Markers:
Elevation: 3-4hrs
Peaks: 14-20 hrs
a. Myo
b. Tn (i)
c. CK
d. AST
e. LD
b
MI Markers:
Elevation: 6-8hrs
Peaks: 18-24 hrs
a. Myo
b. Tn (i)
c. CK
d. AST
e. LD
d
MI Markers:
Elevation: 4-6hrs
Peaks: 12-24 hrs
a. Myo
b. Tn (i)
c. CK
d. AST
e. LD
c
