3.2.3.2 Clinical Enzymology (Aminotransferases/Transaminases, AST/ALT Determination) Flashcards
Specimen consideration for AST/ALT includes:
- Avoid prolonged storage
- Avoid Hemolysis
- Avoid chelating agent
Which of the following does not belong
Avoid chelating agent
In Reitman-Frankel, it is a colorimetric-spectrophotometric assay
T or F
T
Uses 2,4 dinitrophenulhydrazine and 0.4 NaOH
a. Reitman-Frankel
b. Coupled Enzymatic Reaction
a
In Reitman-Frankel If AST is used as catalyst, what is the substrate?
a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate
a
In Reitman-Frankel If AST is used as catalyst, what is the Reagent?
a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate
c
both AST and ALT
In Reitman-Frankel If ALT is used as catalyst, what is the substrate?
a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate
b
In Reitman-Frankel If AST is used as catalyst, what is the Product?
a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate
e
In Reitman-Frankel If ALT is used as catalyst, what is the Product?
a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate
f
Which among is the color developer for Reitman-frankel?
a. 2,4 DNPH
b. 2,4 Dinitrophenylhydrazone
a
Based on Karmen method
a. Reitman-Frankel
b. Coupled Enzymatic Reaction
b
What is the coupling enzyme for AST?
a. Malate dehydrogenase
b. Lactate dehydrogenase
a
What is the coupling enzyme for ALT?
a. Malate dehydrogenase
b. Lactate dehydrogenase
b
What is being produce for coupled Enzymatic reaction?
a. NAD
b. NADH
c. NADP
d. NADPH
a
Reference value:
5-35 U/L
a. AST
b. ALT
a
Reference value:
7-45 U/L
a. AST
b. ALT
b
