3.1.2 Enzymology Intro (Enzyme kinetics)

Question 1

E + S <-> ES <-> P + E

Is this the correct enzymatic reaction?

T or F

Answer 1

T

Question 2

[E] > [S]

a. First-order kinetics
b. zero-order kinetics

Answer 2

a

Question 3

[S] > [E]

a. First-order kinetics
b. zero-order kinetics

Answer 3

b

Question 4

[E] the bracket indicates?

a. Time
b. Concentration

Answer 4

b

Question 5

IF:

[E] > [S]

What is the rate-limiting component?

Answer 5

[S]

Question 6

IF:

[E] > [S]

What should you add to hasten the reaction?

a. Enzyme
b. Substrate

Answer 6

b

Question 7

If there are no available active sites on the enzymes, they will be saturated and cannot continue to reaction

a. First order
b. Zero-order

Answer 7

b

Question 8

Enzyme activity is being measured in Enzyme kinetics and not the concentration

T or F

Answer 8

T

Question 9

V = rate of reaction
Km = [S] at 1/2 Vmax

V = (Vmax . [S]) / (Km + [S])

a. Michaelis-Menten equation
b. Lineweaver-Burke plot

Answer 9

a

Question 10

Point where there are no available active site of enzymes

a. Max velocity
b. 1/2 max velocity

Answer 10

a

Question 11

Linear phase

a. 1st order
b. zero order

Answer 11

a

Question 12

Plateau phase

a. 1st order
b. zero order

Answer 12

b

Question 13

Km = Unique for each Enzymes

T or F

Answer 13

T

Question 14

Double reciprocal plot of the Michaelis-Menten equation

a. Michaelis-Menten equation
b. Lineweaver-Burke plot

Answer 14

b

Question 15

1/v = (Km/Vmax) x (1/[S]) + (1/Vmax)

a. Michaelis-Menten equation
b. Lineweaver-Burke plot

Answer 15

b

Question 16

y = mx + b

1/v

a. y
b. m
c. x
d. b

Answer 16

a

Question 17

y = mx + b

Km/Vmax

a. y
b. m
c. x
d. b

Answer 17

b

Question 18

y = mx + b

1/[s]

a. y
b. m
c. x
d. b

Answer 18

c

Question 19

y = mx + b

1/Vmax

a. y
b. m
c. c
d. b

Answer 19

d