2.5.5 Assessment of Liver Function (Bilirubin determination)) Flashcards by Miguel Acosta | Brainscape

Q

Bilirubin should avoid exposure to light

T or F

A

T

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Q

Colorimetric-spectrophotometrci method for bilirubin is base on what reaction?

a. Benedict’s reaction
b. van den Bergh
c. Schiff’s reaction

A

b

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Q

Diazo reagents
- A = 0.1% sulfanilic acid in HCl
- B = 0.5% sodium nitrite
- Blank = 1.5% HCl

a. Evelyn-Malloy
b. Jendrassik-Grof

A

a

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Q

Diazo reagents:

Sodium acetate
Ascorbic acid
Alkaline tartrate

a. Evelyn-Malloy
b. Jendrassik-Grof

A

b

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Q

Accelerator:

Methanol

a. Evelyn-Malloy
b. Jendrassik-Grof

A

a

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Q

Accelerator

Caffeine

a. Evelyn-Malloy
b. Jendrassik-Grof

A

b

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Q

pH of the reaction:

Alkaline

a. Evelyn-Malloy
b. Jendrassik-Grof

A

b

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Q

pH of the reaction:

Acidic

a. Evelyn-Malloy
b. Jendrassik-Grof

A

a

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Q

Color reaction

Blue

a. Evelyn-Malloy
b. Jendrassik-Grof

A

b

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Q

Color reaction

Red-Purple

a. Evelyn-Malloy
b. Jendrassik-Grof

A

a

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Q

Absorbance maximum:

560nm

a. Evelyn-Malloy
b. Jendrassik-Grof

A

a

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Q

Absorbance maximum:

600nm

a. Evelyn-Malloy
b. Jendrassik-Grof

A

b

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Q

Bilirubin + Diazo + Accelerator → Azodipyrroles

a. Total bilirubin
b. Direct bilirubin
c. Indirect bilirubin

A

a

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Q

Calculated → TB - DB

a. Total bilirubin
b. Direct bilirubin
c. Indirect bilirubin

A

c

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Q

Bilirubin + Diazo → Azodipyrroles

a. Total bilirubin
b. Direct bilirubin
c. Indirect bilirubin

A

b

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Q

Direct spectrometry (Bilirubinometry is based on te absorption of light by bilirubin at

a. 350nm
b. 450nm
c. 550nm
d. 250nm

A

b

Q

Bilirubbinometry is appropriate only for neonatal serum and is not done in adults

T or F

A

T

Due to the presence of caretenoid pigments among adults

Q

Enzymatic method of bilirubin involves oxidation of bilirubin to _______ by bilirubin oxidase

A

Biliverdin

Final color: Colorless

Q

Reference value

0.2-0.8 mg/dL

a. B1
b. B2
c. Total

A

a

Q

Reference value

0-0.2 mg/dL

a. B1
b. B2
c. Total

A

b

Q

Reference value

0.2-1.0 mg/dL

a. B1
b. B2
c. Total

A

c