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MTAP 1 Clinical Chemistry > 2.1.6 Carbohydrates (Chemical Methods) > Flashcards

2.1.6 Carbohydrates (Chemical Methods) Flashcards

1
Q

In copper reduction, what is the common initial reaction for it?

a. Beer’s reaction
b. Copper reaction
c. Benedict’s reaction
d. Lamberts reaction

A

c

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2
Q

Benedict’s reaction:

The 𝐂𝐮𝟐+ in copper sulfate are reduced by ________ and other
reducing agents to 𝐂𝐮+ in the presence of _______ and an/a _______
environment

Choices:
- glucose
- heat
- alkaline

A

Glucose

Heat

Alkaline

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3
Q

Copper reduction is a specific test because glucose is the only one that can catalyze the initial reaction

T or F

A

F

Non specific test since glucose is not the only one that causes this reaction

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4
Q

Ci(+) + Phosphomolybdic acid —-> Phosphomolybdenum

a. Folin-Wu
b. Nelson-Somogyi
c. Neocuproine

A

a

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5
Q

Ci(+) + arseonmolybdic acid —-> arsenomolybdenum

a. Folin-Wu
b. Nelson-Somogyi
c. Neocuproine

A

b

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6
Q

Folin-Wu and Nelson-Somogyi is a ________ reaction

a. Simple complex formation
b. Redox reaction
c. Transferases
d. Hydrolayses

A

b

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7
Q

Phosphomildbdenum and Arsenomolybdenum Produces _______ color upon reaction

a. Yellow
b. Blue
c. Green
d. Red

A

b

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8
Q

The color produced by Phosphomildbdenum, Arsenomolybdenum, and neocuproine is directly proportional to the level of Cuprous ions

T or F

A

T

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9
Q

Neocuproine is a simple complex formation between Cuprous ion and
neocuproine

T or F

A

T

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10
Q

Cuprous-neocuproine complex is yellow-orange in color

a. Blue-Green
b. Yellow-orange
c. Red-orange
d. Violet

A

b

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11
Q

Ferricyanide is reduced by _________ to ferrocyanide

A

Glucose

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12
Q

Ferricyanide (Colorless) is reduced to ferrocyanide (yellow-orange)

T or F

A

F

Baligtad color

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13
Q

Ferric Reduction is also called as Inverse colorimetry or _____________

a. Hagedorn Jensen method
b. Velvet Crow method
c. Crevanille method
d. Schiff’s method

A

a

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14
Q

Most specific, popular, and bes among all chemical methods

a. Copper reduction
b. Ferric Redcution
c. Condensation

A

c

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15
Q

Condensation is affected by oxidizing and reducing agent

T or F

A

F

Not affected

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16
Q

Glucose + o-toluidine ——> Schriff’s base (green)

a. Copper reduction
b. Ferric Redcution
c. Condensation

A

c

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17
Q

In condensation, the color is direclty proportional to the level of glucose

T or F

A

T

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18
Q

Which of this is not chemical method?

a. Copper reduction
b. Hexokinase
c. Condensation
d. Ferric reduction

A

b

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19
Q

Most popular and most commonly used enzymatic method?

a. Glucose oxidase
b. Hexokinase
c. Glucose dehydrogenase

A

a

20
Q

β-D-glucose + O2 + H2O –(Glucose oxidase)–> Gluconic acid + H2O2

a. Glucose oxidase
b. Hexokinase
c. Glucose dehydrogenase

A

a

21
Q

Oxidizing agent for Glucose oxidase?

A

Hydrogen peroxide

22
Q

H2O2 + chromogen oxidized –(Peroxidase)–>
chromogen + H2O

a. Peroxidase-couple/Trinder
b. Polargraphic

A

a

23
Q

Measurement of the degree of O2 consumption using a pO2 (electrode)

a. Peroxidase-couple/Trinder
b. Polargraphic

A

b

24
Q

Commonly used detection technique in Clinical Chemistry

a. Peroxidase-couple/trinder reaction
b. Polarographic

A

a

25
Q

Most common chromagen in Trinder reaction?

a. 4-aminophenozone
b. 4-aminophenol
c. 4-amino-benzaldehyde
d. 4-amino acid

A

a

26
Q

Most common reducing agent that can cause False decrease

A

Ascorbic acid

27
Q

Strong Oxidizing agent in trinders reaction causes false _______

A

Increase

28
Q

H2O2 + 4-aminophenozone –(Peroxidase)—> quinonimine + H2O

What is the color of quinonimine and its absorbance?

A

red purple

500nm

29
Q

Polargraphic is not affected by reducing agent

T or F

A

T

30
Q

Measures the amount of oxygen consumed in the reaction by detecting the amount of oxygen left

a. Trinder reaction
b. Polarographic

A

a

31
Q

Glucose + ATP –(hexokinase)–> glucose-6-PO4 +ADP

Gucose-6-PO4 +NADP –(G6PD)–> 6-PG+ NADPH

a. Trinder reaction
b. Hexokinase
c. Glucose dehydrogenase

A

b

32
Q

Reference method for Hexokinase is coupling reaction which is highly specific

T or F

A

T

33
Q

If hemolyzed / Icteric sample is used for Hexokinase, this cause False ________

A

decrease

34
Q

Involves phosphorylation of Glucose

a. Glucose oxidase
b. Hexokinase
c. Glucose dehydrgenase

A

b

35
Q

What is the coupling enzyme for Hexokinase?

A

G6PD

36
Q

The end product of Hexokinase is NADPH

T or F

A

T

37
Q

What is the absorbance of NADPH? colorimetric or non colorimetric?

A

340nm

Noncolorimetric (Since it is UV)

38
Q

Glucose + NAD –(Glucose dehydrogenase) –> Gluconolactone + NADH

a. Glucose oxidase
b. Hexokinase
c. Glucose dehydrgenase

A

c

39
Q

Glucose dehydrogenase is a non colorimetric method

T or F

A

T

40
Q

Absorbance of NADH

A

340nm

41
Q

Since glucose dehydrogenase is a non-colorimetric method, what dye is used to enable colorimetric spectrophotometric measurement?

a. Formazan dye
b. Zark’s dye
c. Methylene blue
d. Tetrazolium dye

A

d. Tetrazolium dye

42
Q

NADH + tetrazolium –(Diaphorase)–> NAD + formazan

Colorimetric method for Glucose dehydrogenase

T or F

A

T

43
Q

What is the color for formazan?

A

BLue

44
Q

Glucose dehydrogenase:

Highly specific for glucose, not subject to interference from substances normally found in serum and provides results in close agreement with hexokinase method

T or F

A

T

45
Q

Glucose dehydrogenase is not commonly used to except in glucose POCT (Glucometer)

T or F

A

T

MTAP 1 Clinical Chemistry (97 decks)

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